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Physical mapping of a region in the soybean (Glycine max) genome containing duplicated sequences

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Abstract

Pulsed-field gel electrophoresis (PFGE) was used to study a cluster of molecular markers in the soybean genome. There were 550 kb per centimorgan (cM) in the cluster, which is close to the calculated average for the whole genome. The analysis was complicated by the presence of duplicated sequences, and some ambiguities arising from this were resolved by using second-dimension conventional electrophoresis to relate physical maps to the RFLP map of soybean. The results show that there is a high degree of conservation of ‘rare cutter’ sites between homoeologous regions. Finally, PFGE can confirm physical linkage of monomorphic copies of markers, which can aid in the study and comparison of homoeologous regions that are invisible to RFLP analysis.

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References

  1. Blackhall NW, Hammatt N, Davey MR: Analysis of variation in the DNA content of Glycine species: A flow cytometric study. Soybean Genet News 18: 194–200 (1991).

    Google Scholar 

  2. Burmeister M: Strategies for mapping large regions of mammalian genomes. In: Burmeister M, Ulanovsky L (eds) Methods in Molecular Biology, vol. 12: Pulsed-field gel electrophoresis, pp. 259–284. Humana Press, Totowa, NJ (1992).

    Google Scholar 

  3. Carroll BJ, McNeil DL, Gresshoff PM: A supernodulation and nitrate tolerant symbiotic (nts) soybean mutant. Plant Physiol 78: 34–40 (1985).

    Google Scholar 

  4. Carroll BJ, McNeil DL, Gresshoff PM: Isolation and properties of soybean mutants which nodulate in the presence of high nitrate concentrations. Proc Natl Acad Sci USA 82: 4162–4166 (1985).

    Google Scholar 

  5. Cheung WY, Chao S, Gale MD: Long-range physical mapping of the A-amylase-1 (A-Amy-1) loci on homoeologous group 6 chromosomes of wheat. Mol Gen Genet 229: 373–379 (1991).

    Article  PubMed  Google Scholar 

  6. Cho T, Davies CS, Nielsen NC: Inheritance and organization of glycinin genes in soybean. Plant Cell 1: 329–337 (1989).

    Article  PubMed  Google Scholar 

  7. Ganal MW, Young ND, Tanksley SD: Pulsed field gel electrophoresis and physical mapping of large DNA fragments in the Tm-2a region of chromosome 9 in tomato. Mol Gen Genet 215: 395–400 (1989).

    Google Scholar 

  8. Gurley WB, Hepburn AG, Key JL: Sequence organization of the soybean genome. Biochim Biophys Acta 561: 167–183 (1979).

    PubMed  Google Scholar 

  9. Hadley HH, Hymowitz T. In: Cadwell BE (ed) Soybeans: Improvement, Production, and Uses, pp. 97–114. American Society of Agronomy, Madison, WI (1973).

    Google Scholar 

  10. Helentjaris T, Weber D, Wright S: Identification of the genomic locations of duplicate nucleotide sequences in maize by analysis of restriction fragment length polymorphisms. Genetics 118: 353–363 (1988).

    Google Scholar 

  11. Honeycutt RJ, Sobral BWS, McClelland M, Atherly AG: Analysis of large DNA from soybean (Glycine max L. Merr.) by pulsed-field gel electrophoresis. Plant J 2: 133–135 (1992).

    PubMed  Google Scholar 

  12. Keim P, Shoemaker RC, Palmer RG: Restriction fragment length polymorphism diversity in soybean. Theor Appl Genet 77: 786–792 (1989).

    Article  Google Scholar 

  13. Landau-Ellis D, Angermüller S, Shoemaker R, Gresshoff PM: The genetic locus controlling supernodulation in soybean (Glycine max. L.) co-segregates tightly with a cloned molecular marker. Mol Gen Genet 228: 221–226 (1991).

    Article  PubMed  Google Scholar 

  14. 14. Landau-Ellis D, Gresshoff PM: Supernodulating soybean mutant alleles, nts382 and nts1007, show no recombination with the same restriction fragment length polymorphism marker. Mol Plant-Microbe Inter, (1992).

  15. Lee JS, Verma DPS: Structure and chromosomal arrangement of leghemoglobin genes in kidney bean suggest divergence in soybean leghemoglobin gene loci following tetraploidization. Eur Mol Biol Org J 3: 2745–2752 (1984).

    Google Scholar 

  16. New England Biolabs Catalog 1992, p. 190.

  17. Schwartz DC, Cantor CR: Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell 37: 67–75 (1984).

    Article  PubMed  Google Scholar 

  18. Segal G, Sarfatti M, Schaffer MA, Ori N, Zami D, Fluhr R: Correlation of genetic and physical structure in the region surrounding the I 2 Fusarium oxysporum resistance locus in tomato. Mol Gen Genet 231: 179–185 (1992).

    PubMed  Google Scholar 

  19. Shoemaker RC, Guffy RD, Lorenzen LL, Specht JE: Molecular genetic mapping of soybean: map utilization. Crop Sci 2: 1091–1098 (1992).

    Google Scholar 

  20. Singh RJ, Hymowitz T: The genomic relationship between Glycine max (L.) Merr. and Glycine soja (Sieb. and Zucc.) as revealed by pachytene chromosome analysis. Theor Appl Genet 76: 705–711 (1988).

    Article  Google Scholar 

  21. Walter MA, Cox DW: Two-dimensional DNA electrophoresis (2D-DE) for mammalian DNA. In: Burmeister M, Ulanovsky L (eds) Methods in Molecular Biology, vol. 12: Pulsed-Field Gel Electrophoresis, pp. 285–297. Humana Press, Totowa, NJ (1992).

    Google Scholar 

  22. Wicking C, Williamson B: From linked marker to gene. Trends Genet 7: 288–293 (1991).

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Plant Molecular Genetics and Center for Legume Research, University of Tennessee, 269 Ellington Plant Sciences Building, 37901-1071, Knoxville, TN, USA

    Roel P. Funke, Alexander Kolchinsky & Peter M. Gresshoff

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  1. Roel P. Funke
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  2. Alexander Kolchinsky
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  3. Peter M. Gresshoff
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Funke, R.P., Kolchinsky, A. & Gresshoff, P.M. Physical mapping of a region in the soybean (Glycine max) genome containing duplicated sequences. Plant Mol Biol 22, 437–446 (1993). https://doi.org/10.1007/BF00015974

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  • DOI: https://doi.org/10.1007/BF00015974

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