Abstract
A root-specific cDNA clone, PVR3, was isolated from a bean (Phaseolus vulgaris L.) root cDNA library by a differential screening procedure. The nucleotide sequence of PVR3 contains an open reading frame coding for an 11.14 kDa polypeptide of 102 amino acid residues; the first 25 amino acids correspond to the sequence characteristic of a signal peptide. Comparison of the deduced PVR3 polypeptide sequence with the polypeptide sequences of previously cloned genes indicates that PVR3 may encode a ns-LTP-like protein. Molecular modelling of the PVR3 protein predicts that it has a three-dimensional structure that is similar to the three-dimensional model determined from the maize ns-LTP. The PVR3 mRNA accumulated mainly in the roots of young seedlings. It can be detected at low levels in flowers, but it is not detected in other organs. Genomic Southern blot analysis indicates that the genomic DNA corresponding to PVR3 cDNA is encoded by a single gene or small gene family in the bean genome.
This is a preview of subscription content, log in via an institution to check access.
References
Bernhard WR, Thoma S, Botella J, Somerville CR: Isolation of a cDNA clone for spinach lipid transfer protein and evidence that the protein is synthesized by the secretory pathway. Plant Physiol 95: 164–170 (1991).
Chasan R: Lipid transfer proteins: moving molecules? Plant Cell 3: 842–843 (1991).
Chomczynski P, Sacchi N: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156–159 (1987).
Conkling MA, Cheng C-L, Yamamoto YT, Goodman HM: Isolation of transcriptionally regulated root-specific genes from tobacco. Plant Physiol 93: 1203–1211 (1990).
Dieryck W, Gautier M-F, Lullien V, Joudrier P: Nucleotide sequence of a cDNA encoding a lipid transfer protein from wheat (Triticum durum Desf.). Plant Mol Biol 19: 707–709 (1992).
Esau K: Anatomy of Seed Plants, 2nd ed. John Wiley, New York (1977).
Fleming AJ, Mandel T, Hofmann S, Sterk P, De Vries SC, Kuhlemeier C: Expression pattern of a tobacco lipid transfer protein gene within the shoot apex. Plant J 2: 855–862 (1992).
Foster GD, Robinson SW, Blundell RP, Roberts MR, Hodge R, Draper J, Scott RJ: A Brassica napus mRNA encoding a protein homologous to phospholipid transfer proteins, is expressed specifically in the tapetum and developing microspores. Plant Sci 84: 187–192 (1992).
Jakobsen K, Klemsdal SS, Aalen RB, Bosnes M, Alexander D, Olesen O-A: Barley aleurone cell development: molecular cloning of aleurone-specific cDNAs from immature grains. Plant Mol Biol 12: 285–293 (1989).
John I, Wang H, Held BM, Wurtele ES, Colbert JT: An mRNA that specifically accumulates in maze roots delineates a novel subset of developing cortical cells. Plant Mol Biol 20: 821–831 (1992).
Kotilainen M, Helariutta Y, Elomaa P, Paulin L, Teeri TH: A corolla- and carpel-abundant, non-specific lipid transfer protein gene is expressed in the epidermis and parenchyma of Gerbera hybrida var. Regina (Compositae). Plant Mol Biol 26: 971–978 (1994).
Krause A, Sigrist CJA, Dehning I, Sommer H, Broughton WJ: Accumulation of transcripts encoding a lipid transfer-like protein during deformation of nodulation-competent Vigna unguiculata root hairs. Mol Plant-Microbe Interact 7: 411–418 (1994).
Molina A, Segura A, Garcia-Olmedo F: Lipid transfer proteins (nsLTPs) from barley and maize leaves are potent inhibitors of bacterial and fungal plant pathogens. FEBS Lett 316: 119–122 (1993).
Pyee J, Yu H, Kolattukudy PE: Identification of a lipid transfer protein as the major protein in the surface wax of broccoli (Brassica oleracea) leaves. Arch Biochem Biophys 311: 460–468 (1994).
Schiefelbein JW, Benfey PN: The development of plant roots: new approaches to underground problems. Plant Cell 3: 1147–1154 (1991).
Shin DH, Lee JY, Hwang KY, Kim KK, Suh SW: High-resolution crystal structure of the non-specific lipid-transfer protein from maize seedlings. Structure 3: 189–199 (1995).
Sossountzov L, Ruiz-Avila L, Vignols F, Jolliot A, Arondel V, Tchang F, Grosbois M, Guerbette F, Miginiac E, Delseny M, Puigdomenech P, Kader J-C: Spatial and temporal expression of a maize lipid transfer protein gene. Plant Cell 3: 923–933 (1991).
Sterk P, Booij H, Schellekens GA, van Kammen A, de Vries SC: Cell-specific expression of the carrot EP2 lipid transfer protein gene. Plant Cell 3: 907–921 (1991).
Taylor BH, Scheuring CF: A molecular maker for lateral root initiation: the RSI-1 gene of tomato (Lycopersicon esculentum Mill) is activated in early lateral root primodia. Mol Gen Genet 243: 148–157 (1994).
Tchang F, This P, Stiefel V, Arondel V, Morch M-D, Pages M, Puigdomenech P, Grellet F, Delseny M, Bouillon P, Huet J-C, Guerbette F, Beavais-Cante F, Duranton H, Pernollet J-C, Kader J-C: Phospholipid transfer protein: full-length cDNA and amino acid sequence in maize. J Biol Chem 263: 16849–16855 (1988).
Terras FRG, Goderis IJ, Van Leuven F, Vanderleyden J, Cammue BPA, Broekaert WF: In vitro antifungal activity of a radish (Raphanus sativus L.) seed protein homologous to nonspecific lipid transfer proteins. Plant Physiol 100: 1055–1058 (1992).
Thoma S, Hecht U, Kippers A, Botella J, de Vries S, Somerville C: Tissue-specific expression of a gene encoding a cell wall-localized lipid transfer protein from Arabidopsis. Plant Physiol 105: 35–45 (1994).
Thoma S, Kaneko Y, Somerville C: A non-specific lipid transfer protein from Arabidopsis is a cell wall protein. Plant J 3: 427–436 (1993).
Torres-Schumann S, Godoy JA, Pintor-Toto JA: A probable lipid transfer protein gene is induced by NaCl in stems of tomato plants. Plant Mol Biol 18: 749–757 (1992).
Tsuboi S, Suga T, Takishima K, Mamiya G, Matsui K, Ozeki Y, Yamada M: Organ-specific occurrence and expression of the isoforms of nonspecific lipid transfer protein in castor bean seedlings, and molecular cloning of a full-length cDNA for a cotyledon-specific isoform. J Biochem 110: 823–831 (1991).
von Heijne GV: A new method for predicting signal sequence cleavage sites. Nucl Acids Res 14: 4683–4691 (1986).
Wirtz KWA: Phospholipid transfer proteins. Annu Rev Biochem 60: 73–99 (1991).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Choi, DW., Song, J.Y., Oh, MH. et al. Isolation of a root-specific cDNA encoding a ns-LTP-like protein from the roots of bean (Phaseolus vulgaris L.) seedlings. Plant Mol Biol 30, 1059–1066 (1996). https://doi.org/10.1007/BF00020816
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00020816