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Regulation of the chitinase gene expression in suspension-cultured rice cells by N-acetylchitooligosaccharides: differences in the signal transduction pathways leading to the activation of elicitor-responsive genes

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Abstract

Expression patterns of chitinase transcripts induced by N-acetylchitooligosaccharide elicitor were analyzed by northern blot hybridization in order to reveal a signal transduction pathway leading to the activation of class I chitinase genes (Cht-1 and Cht-3), which may play an important role in producing N-acetylchitooligosaccharide elicitor. The transcription level of both genes was enhanced in response to N-acetylchitooligosaccharides larger than pentaose at subnanomolar concentrations. These structure and dose dependencies were consistent not only with those for a 75 kDa high-affinity binding protein for N-acetylchitooligosaccharide elicitor in the plasma membrane, but also with other series of cellular responses including phytoalexin production and the expression of elicitor-responsive genes (EL2, EL3). Therefore, the elicitor signal to evoke these cellular responses including the activation of the chitinase genes could be common and transmitted into cells through the 75 kDa protein. However, the signal transduction pathway for the activation of the chitinase gene appeared to diverge from those for the other elicitor-responsive genes shortly after the signal perception. It was shown that the induction of chitinase expression by N-acetylchitooligosaccharide would require protein phosphorylation, but not de novo protein synthesis. The oxidative burst was demonstrated not to be necessary for transcriptional induction of the all four elicitor-responsive genes (Cht, PAL, EL2, EL3) by N-acetylchitooligosaccharide.

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Author notes

  1. Akira Kawakami

    Present address: Department of Low-temperature Science, Hokkaido National Agricultural Experiment Station, Hitsujigaoka, Toyohira-ku Sapporo, 062-8555, Japan

  2. Tadaaki Hibi

    Present address: Laboratory of Plant Pathology, University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

Authors and Affiliations

  1. Department of Biotechnology, National Institute of Agrobiological Resources, 2-1-2 Kannondai, Tsukuba, 305-8602, Japan

    Yoko Nishizawa, Dao-Yao He, Naoto Shibuya & Eiichi Minami

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  1. Yoko Nishizawa
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  2. Akira Kawakami
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  3. Tadaaki Hibi
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  4. Dao-Yao He
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  5. Naoto Shibuya
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  6. Eiichi Minami
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Nishizawa, Y., Kawakami, A., Hibi, T. et al. Regulation of the chitinase gene expression in suspension-cultured rice cells by N-acetylchitooligosaccharides: differences in the signal transduction pathways leading to the activation of elicitor-responsive genes. Plant Mol Biol 39, 907–914 (1999). https://doi.org/10.1023/A:1006161802334

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