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Introduction of the reconstructed yeast ferric reductase gene, refre1, into tobacco

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Abstract

Fe(III) reduction at the root surface is an obligatory step in Fe uptake used by Strategy-I plants. The genes FRE1 and FRE2 are responsible for a similar mechanism of Fe acquisition in the cell membrane of Saccharomyces cerevisiae. We introduced the FRE1 gene into tobacco plants (Nicotiana tabacum L. cv. SR1). However, the transgenic tobacco showed no additional reductase activity, because the FRE1 transcripts from this transgenic tobacco were shorter than expected. Further investigation revealed that the coding region of the FRE1 gene was polyadenylated. We then reconstructed the whole sequence of the FRE1 gene and named it refre1 (reconstructed FRE1). The refre1 gene was introduced into tobacco plants. The transgenic plants carrying the refre1 gene produced full length mRNA and had constitutive ferric reductase activity.

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Authors and Affiliations

  1. Laboratory of Plant Molecular Physiology, Department of Applied Biological Chemistry, The University of Tokyo, 113-8657, Tokyo, Japan

    Hiroyuki Oki, Hiromi Nakanishi & Satoshi Mori

  2. Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation, 2-1-6 Sengen, 305-0047, Tsukuba, Japan

    Hirotaka Yamaguchi & Satoshi Mori

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  1. Hiroyuki Oki
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  2. Hirotaka Yamaguchi
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  3. Hiromi Nakanishi
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  4. Satoshi Mori
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Oki, H., Yamaguchi, H., Nakanishi, H. et al. Introduction of the reconstructed yeast ferric reductase gene, refre1, into tobacco. Plant and Soil 215, 211–220 (1999). https://doi.org/10.1023/A:1004591012061

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