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P680+ reduction in oxygen-evolving Photosystem II core complexes

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Abstract

The kinetics of P680+ reduction in oxygen-evolving spinach Photosystem II (PS II) core particles were studied using both repetitive and single-flash 830 nm transient absorption. From measurements on samples in which PS II turnover is blocked, we estimate radical-pair lifetimes of 2 ns and 19 ns. Nanosecond single-flash measurements indicate decay times of 7 ns, 40 ns and 95 ns. Both the longer 40 ns and 95 ns components relate to the normal S-state controlled Yz → P680+ electron transfer dynamics. Our analysis indicates the existence of a 7 ns component which provides evidence for an additional process associated with modified interactions involving the water-splitting catalytic site. Corresponding microsecond measurements show decay times of 4 μs and 90 μs with the possibility of a small component with a decay time of 20–40 μs. The precise origin of the 4 μs component remains uncertain but appears to be associated with the water-splitting center or its binding site while the 90 μs component is assigned to P680+-QA − recombination. An amplitude and kinetic analysis of the flash dependence data gives results that are consistent with the current model of the oxygen-evolving complex.

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Abbreviations

PS II-:

Photosystem II-

P680-:

primary donor (Chl-aII dimer) of PS II

Yz-:

Tyr 161 donor to P680

QA-:

quinone secondary acceptor to P680

LHC-:

light-harvesting chlorophyll protein of PS II

BBY-:

Berthold, Babcock and Yocum PS II membrane fragment preparation

PPBQ-:

phenyl-p-benzoquinone

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Authors and Affiliations

  1. School of Physics, University of Sydney, 2006, NSW, Australia

    P. B. Lukins & P. J. Walker

  2. School of Biological Sciences, University of Sydney, 2006, NSW, Australia

    Adele Post & A. W. D. Larkum

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  1. P. B. Lukins
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  2. Adele Post
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  3. P. J. Walker
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  4. A. W. D. Larkum
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Lukins, P.B., Post, A., Walker, P.J. et al. P680+ reduction in oxygen-evolving Photosystem II core complexes. Photosynth Res 49, 209–221 (1996). https://doi.org/10.1007/BF00034782

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  • DOI: https://doi.org/10.1007/BF00034782

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