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Fluorometric equipment for monitoring P680+• reduction in PS II preparations and green leaves

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Abstract

Newly developed equipment is described that permits the monitoring of laser flash induced transients of the normalised chlorophyll-a fluorescence quantum yield in isolated PS II preparations and whole leaves with a high time resolution. The essential operational unit of the set-up is a rapidly gated photomultiplier. In this way, the fluorescence artefact, due to the high intensity excitation laser flash, is sufficiently suppressed and the dead time of the signal response is reduced to about 500 ns. It is shown that the fluorescence rise kinetics in the μs time-domain, after flash excitation is strongly dependent on the redox state of the primary electron donor of PS II (P680). At high excitation energies, the decay of carotenoid triplets, which are very efficient quenchers of chlorophyll singlet states, dominates the rise kinetics of the flash induced fluorescence yield in the μs time domain.

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Authors and Affiliations

  1. Max-Volmer-Institut für Biophysikalische Chemie und Biochemie, Technische Universität Berlin, Strae des 17. Juni 135, D-10623, Berlin, Germany

    Frank Reifarth, Gert Christen & Gernot Renger

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  1. Frank Reifarth
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  2. Gert Christen
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  3. Gernot Renger
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Reifarth, F., Christen, G. & Renger, G. Fluorometric equipment for monitoring P680+• reduction in PS II preparations and green leaves. Photosynthesis Research 51, 231–242 (1997). https://doi.org/10.1023/A:1005881810057

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  • DOI: https://doi.org/10.1023/A:1005881810057

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