Abstract
Carrot somatic embryogenesis was serially observed using a cell cluster immobilizing system with Phytagel. Embryogenic cell clusters ranging in size from 32 to 63 μm were collected by filtration and used for somatic embryo induction. Of the 432 cell clusters, 253 grew, i.e., the size of these cell clusters increased, and 192 developed into globular embryos. Through serial observation, the number of somatic embryos produced from each cell cluster was identified. Cell clusters which developed into two or more embryos grew and developed slowly as compared with cell clusters which developed into single embryos. Serial observation also revealed that some cell clusters consisted of several parts, each of which independently grew as separate units. In cases where two growing parts fused into one embryo, morphological abnormalities such as curvature or lumps in their bodies were occasionally observed.
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References
Chi C-M, Vits H, Staba EJ, Cooke TJ & Hu W-S (1994) Morphological kinetics and distribution in somatic embryo cultures. Biotech. Bioeng. 44: 368–378
Dunstan DI, Bethune TD & Abrams SR (1991) The use of (±)-abscisic acid and (±)-abscisyl alcohol in somatic embryo maturation of white spruce (Picea glauca). Plant Sci. 76: 219–228
Dunstan DI, Bethune TD & Bock CA (1993) Somatic embryo maturation from long-term suspension cultures of white spruce (Picea glauca). In Vitro Cell Dev. Biol. 29P: 109–112
Fujimura T & Komamine A (1980) The serial observation of embryogenesis in a carrot cell suspension culture. New Phytol. 86: 213–218
Gamborg OL (1970) The effects of amino acid and ammonium on the growth of plant cells in suspension culture. Plant Physiol. 45: 372–375
Halperin W (1966) Alternative morphogenetic events in cell suspensions. Am. J. Bot. 53: 443–453
Ibaraki Y, Shimazu T & Kurata K (1996) Relationship between shape of carrot somatic embryos and their growth and development. Acta Hort. 440: 549–554
Liyanage KH & Kurata K (1992) Relation between the shape of carrot somatic embryos and their quality plant regeneration. Acta Hort. 319: 267–272
Maës O, Coutos-Thevenot P, Jouenne T, Boulay M & Guern J (1997) Influrence of extracellular proteins, proteases and protease inhibitors on grapevine somatic embryogenesis. Plant Cell Tiss. Org. Cult. 50: 97–105
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tabacco tissue cultures. Physiol. Plant 15: 473–497
Nomura K & Komamine A (1985) Identification and isolation of single cells that produce somatic embryos at a high frequency in a carrot suspension culture. Plant Physiol. 79: 988–991
Osuga K, Kamada H & Komamine A (1993) Cell density is an important factor for synchronization of late stages of somatic embryogenesis at high frequency. Plant Tiss. Cult. Lett. 10: 180–183
Osuga K & Komamine A (1994) Synchronization of somatic embryogenesis from carrot cells at high frequency as a basis for the mass production of embryos. Plant Cell Tiss. Org. Cult. 39: 125–135
Padmanabhan K, Cantliffe DJ, Harrell RC & McConnell DB (1998) A comparison of shoot-forming and non-shoot-forming somatic embryos of sweet potato [Ipomoea batatas (L.) Lam.] using computer vision and histological analysis. Plant Cell Rep. 17: 685–692
Toonen MAJ, Hendriks T, Schmidt EDL, Verhoeven HA, van Kammen AB & de Vries SC (1994) Description of somatic-embryoforming single cells in carrot suspension cultures employing video cell tracking. Planta 194: 565–572
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Ibaraki, Y., Matsushima, R. & Kurata, K. Analysis of morphological changes in carrot somatic embryogenesis by serial observation. Plant Cell, Tissue and Organ Culture 61, 9–14 (2000). https://doi.org/10.1023/A:1006484023765
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DOI: https://doi.org/10.1023/A:1006484023765