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Plant regeneration from embryogenic cell suspension-derived protoplasts of rubber

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Abstract

Embryogenic cell suspensions of rubber derived from immature inflorescences and inner integuments of immature fruits released 3.1 ± 0.2 × 107 protoplasts g-1 f. wt. (mean ± s.e.m, n = 10) and 3.2 ± 0.2 × 107 protoplasts g-1 f. wt., with mean viabilities of 83 ± 2% and 77 ± 8%, respectively. Sustained mitotic division was observed only when protoplasts were cultured in KPR liquid medium on nitrocellulose membranes overlying the same semi-solid medium containing Lolium multiflorum nurse cells. Protoplast-derived cell colonies were produced within 2 months of culture. Protoplast-derived cell colonies proliferated, upon subculture to MS-based regeneration medium, with 40% of the protoplast-derived calli developing somatic embryos. The latter germinated into plants on the same medium after 3 months of culture.

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Authors and Affiliations

  1. Biotechnology Division, Rubber Research Institute of India, Kottayam, 686 009, Kerala, India

    S. Sushamakumari & M.P. Asokan

  2. Plant Science Division, School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, UK

    P. Anthony, K.C. Lowe, J.B. Power, M.R. Davey & M.R. Davey

Authors
  1. S. Sushamakumari
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  2. M.P. Asokan
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  3. P. Anthony
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  4. K.C. Lowe
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  5. J.B. Power
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  6. M.R. Davey
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Corresponding author

Correspondence to M.R. Davey.

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Sushamakumari, S., Asokan, M., Anthony, P. et al. Plant regeneration from embryogenic cell suspension-derived protoplasts of rubber. Plant Cell, Tissue and Organ Culture 61, 81–85 (2000). https://doi.org/10.1023/A:1006494404224

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  • DOI: https://doi.org/10.1023/A:1006494404224

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