Summary
A spot agglutination test was standardised for the rapid diagnosis of goat pox using antigen and antibody systems after being coupled to glutaraldehyde-treated sheep erythrocytes. The sensitivity was comparable to that of counter-immuno-electrophoresis and reverse-phase passive haemagglutination tests. The spot agglutination test was found to be very simple, easy to perform, rapid and economical for the detection of goat pox antigen and antibody and can be performed under field conditions.
Résumé
Un test d'agglutination pour le diagnostic rapide de la variole caprine a été standardisé en utilisant un système anticorps/antigène couplé à des érythrocytes de mouton traités au glutaraldehyde. La sensiblité fut comparable aux tests d'haemagglutination passive en phase inverse ou par comptage en imuno-électrophorèse. Ce test d'agglutination s'est montré très simple et facile d'utilisation, rapide et économique dans la détection d'antigène ou d'anticorps lors de la variole caprine, de plus il peut être utilisé dans des conditions de terrain.
Resumen
Se estandarizó un test de aglutinación para el diagnóstico rápido de la viruela caprina utilizando complejos antígeno-anticuerpo fijados a eritrocitos de oveja previamente tratados con glutaraldehído. La sensibilidad del test fue comparable a la del test de contrainmunoelectroforesis y a la del test de hemoaglutinación pasiva de fase inversa. El test de hemoaglutinación resultó ser muy sencillo, fácil de realizar, rápido y económico para detectar antígenos y anticuerpos de la viruela caprina y puede ser realizado en condiciones de campo.
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Tiwari, A.K., Rao, T.V.S. & Negi, B.S. Spot agglutination test for the rapid diagnosis of goat pox. Trop Anim Health Prod 28, 213–215 (1996). https://doi.org/10.1007/BF02240937
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DOI: https://doi.org/10.1007/BF02240937