Skip to main content
Log in

Spot agglutination test for the rapid diagnosis of goat pox

Test d'Agglutination Pour Le Diagnostic Rapide De La Variole Caprine

Test De Aglutinacion Para El Diagnostico Rapido De La Viruela Caprina

  • Published:
Tropical Animal Health and Production Aims and scope Submit manuscript

Summary

A spot agglutination test was standardised for the rapid diagnosis of goat pox using antigen and antibody systems after being coupled to glutaraldehyde-treated sheep erythrocytes. The sensitivity was comparable to that of counter-immuno-electrophoresis and reverse-phase passive haemagglutination tests. The spot agglutination test was found to be very simple, easy to perform, rapid and economical for the detection of goat pox antigen and antibody and can be performed under field conditions.

Résumé

Un test d'agglutination pour le diagnostic rapide de la variole caprine a été standardisé en utilisant un système anticorps/antigène couplé à des érythrocytes de mouton traités au glutaraldehyde. La sensiblité fut comparable aux tests d'haemagglutination passive en phase inverse ou par comptage en imuno-électrophorèse. Ce test d'agglutination s'est montré très simple et facile d'utilisation, rapide et économique dans la détection d'antigène ou d'anticorps lors de la variole caprine, de plus il peut être utilisé dans des conditions de terrain.

Resumen

Se estandarizó un test de aglutinación para el diagnóstico rápido de la viruela caprina utilizando complejos antígeno-anticuerpo fijados a eritrocitos de oveja previamente tratados con glutaraldehído. La sensibilidad del test fue comparable a la del test de contrainmunoelectroforesis y a la del test de hemoaglutinación pasiva de fase inversa. El test de hemoaglutinación resultó ser muy sencillo, fácil de realizar, rápido y económico para detectar antígenos y anticuerpos de la viruela caprina y puede ser realizado en condiciones de campo.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Bansal, R. P., Joshi, R. C., Sharma, B. &Chandra, U. (1987). Reverse-phase passive haemagglutination test for the detection of rinderpest antigen.Tropical Animal Health and Production,19, 53–55.

    Google Scholar 

  • Bhambani, B. D. &Krishnamurthy, D. (1963). An immunodiffusion test for the diagnosis of sheep and goat pox.Journal of Comparative Pathology,73, 349–358.

    Google Scholar 

  • Dutta, S. &Soman, J. P. (1990). Dot immunobinding technique for the differential diagnosis of pox diseases of sheep and goats.Indian Journal of Animal Sciences,60, 1186–1187.

    Google Scholar 

  • Matthews, R. E. F. (1982). Classification and nomenclature of viruses.Inter-virology,17, 42–46.

    Google Scholar 

  • Sharma, B., Negi, B. S., Pandey, A. B., Bandyopadhyay, S. K., Shanker, H. &Yadav, M. P. (1988a). Detection of goat pox antigen and antibody by CIE test.Tropical Animal Health and Production,20, 109–113.

    Google Scholar 

  • Sharma, B., Negi, B. S., Yadav, M. P., Shanker, H. &Pandey, A. B. (1988b). Application of ELISA in the detection of goat pox antigen and antibodies.Acta Virologica,32, 65–69.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tiwari, A.K., Rao, T.V.S. & Negi, B.S. Spot agglutination test for the rapid diagnosis of goat pox. Trop Anim Health Prod 28, 213–215 (1996). https://doi.org/10.1007/BF02240937

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02240937

Keywords

Navigation