Abstract
A mixed culture of a chlorobenzoate-(3-CBA)-degradingPseudomonas aeruginosa, strain 3mT, and a phenol/cresols-degradingPseudomonas sp., strain CP4, simultaneously and efficiently degraded mixtures of 3-CBA and phenol/cresols. However, strains 3mT and CP4 usedortho- andmeta-ring cleavage pathways, respectively. Degradation of 3-CBA was complete when the 3-CBA was equal in amount to or less than that of phenol. CP4/3mT inoculum ratios (w/w) of 1:1 or 1:2 gave the most effective degradation of both the substrates in the mixture. The mixed culture degraded equimolar mixtures of 3-CBA/phenol up to 10mm. Equimolar mixtures of 3-CBA ando-, m- orp-cresol were also degraded by the mixed culture.
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References
Ajith-KumarP.V. & KunhiA.A.M. 1993 Mineralization of chlorobenzoic acids byPseudomonas aeruginosa 3mT-the metabolic studies. InAbstract Book, IFCON'93, 3rd International Food Convention p. 48. Mysore, India: Association of Food Scientists and Technologists (India).
ArnowL.E. 1937 Colorimetric determination of the components of 3,4-dihydroxyphenyl alanine-tyrosine mixtures.Journal of Biological Chemistry 118, 531–537.
BabuK.S., Ajith-KumarP.V. & KunhiA.A.M. 1992 Degradation of phenols byPseudomonas sp. strain CP4. InAbstract Book, 33rd Annual Conference, p. 81. Goa, India: Association of Microbiologists of India.
BartelsI., KnackmussH.J. & ReinekeW. 1984 Suicide inactivation of catechol 2,3-dioxygenase fromPseudomonas putida mt-2 by 3-halocatechols.Applied and Environmental Microbiology 47, 500–505.
BaylyR.C. & WigmoreG.J. 1973 Metabolism of phenol and cresols by mutants ofPseudomonas putida.Journal of Bacteriology 113, 1112–1120.
BergmannJ.G. & SanikJrJ. 1957 Determination of trace amounts of chlorine in naphtha.Analytical Chemistry 29, 241–243.
ChaudhryG.R. & ChapalamaduguS. 1991 Biodegradation of halogenated aromatic compounds.Microbiological Reviews 55, 59–79.
DagleyS. 1978 Pathway for the utilization of organic growth substrates. InThe Bacteria, Vol. 6, ed GunsalusI.C. pp. 305–388, London: Academic Press.
DornE. & KnackmussH.J. 1978 Chemical structure and biodegradability of halogenated aromatic compounds. Substituent effects on 1,2-dioxygenation of catechol.Biochemistry Journal 174, 85–94.
EngelhardtG., RastH.C. & WallnoferP.R. 1979 Degradation of aromatic carboxylic acids byNocardia sp. DSM 43251.FEMS Microbiology Letters 4, 245–251.
GibsonD.T., KochJ.R., SchuldC.L. & KallioR.E. 1968 Oxidative degradation of aromatic hydrocarbons by microorganisms. II. Metabolism of halogenated aromatic hydrocarbons.Biochemistry 7, 3795–3802.
HintereggerC., LeitnerR., LoidlM., FerschlA. & StreichsbierF. 1992 Degradation of phenol and phenolic compounds byPseudomonas putida EKII.Applied Microbiology and Biotechnology 37, 252–259.
HorvathR.S. & AlexanderM. 1970 Cometabolism ofm-chlorobenzoate by anArthrobacter.Applied Microbiology 20, 254–258.
KleckaG.M. & GibsonD.T. 1981 Inhibition of catechol-2,3-dioxygenase fromPseudomonas putida by 3-chlorocatechol.Applied and Environmental Microbiology 41, 1159–1165.
LacosteR.J. VenableS.H. & StoneJ.C. 1959 Modified 4-amino-antipyrene colorimetric method for phenols. Applications to an acrylic monomer.Analytical Chemistry 31, 1246–1249.
MorsenA. & RehmH.J. 1990 Degradation of phenol by a defined mixed culture immobilised by adsorption on activated carbon and sintered glass.Applied Microbiology and Biotechnology 33, 206–212.
MurrayK., DugglebyC.J., Sala-TrepatJ.M. & WilliamsP.A. 1972 The metabolism of benzoate and methyl benzoates via themeta-cleavage pathway byPseudomonas arvilla mt-2.European Journal of Biochemistry 28, 301–310.
PettigrewC.A., HaiglerB.E. & SpainJ.C. 1991 Simultaneous biodegradation of chlorobenzene and toluene by aPseudomonas strain.Applied and Environmental Microbiology 57, 157–162.
ReinekeW. & KnackmussH.J. 1988 Microbial degradation of haloaromatics.Annual Review of Microbiology 42, 263–287.
RojoF., PieperD.H., EngesserK.H., KnackmussH.J. & TimmisK.N. 1987 Assemblage ofortho cleavage route for simultaneous degradation of chloro- and methyl-aromatics.Science 238, 1395–1398.
SchmidtE. 1987 Response of a chlorophenols degrading mixed culture to changing loads of phenol, chlorophenol and cresol.Applied Microbiology and Biotechnology 27, 94–99.
SchmidtE., HellwigM. & KnackmussH.J. 1983 Degradation of chlorophenols by a defined mixed microbial community.Applied and Environmental Microbiology 46, 1038–1044.
SpokesJ.R. & WalkerN. 1974 Chlorophenol and chlorobenzoic acids co-metabolism by different genera of soil bacteria.Archives of Microbiology 96, 125–134.
TaegerK., KnackmussH.J. & SchmidtE. 1988 Biodegradability of mixtures of chloro- and methyl-substituted aromatics: simultaneous degradation of 3-chlorobenzoate and 3-methylbenzoate.Applied Microbiology and Biotechnology 28, 603–608.
WasserfallenA., RekikM. & HarayamaS. 1991 APseudomonas putida strain able to degradem-toluate in the presence of 3-chlorocatechol.Biotechnology 9, 296–298.
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The authors are with the Microbiology and Bioengineering Department, Central Food Technological Research Institute, Mysore-570013, India;
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Babu, K.S., Ajith-Kumar, P.V. & Kunhi, A.A.M. Simultaneous degradation of 3-chlorobenzoate and phenolic compounds by a defined mixed culture ofPseudomonas spp.. World J Microbiol Biotechnol 11, 148–152 (1995). https://doi.org/10.1007/BF00704636
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DOI: https://doi.org/10.1007/BF00704636