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Autoradiographic investigation of schirrous carcinoma of the human stomach by culture in diffusion chambers

  • Oncology
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Abstract

When a schirrous carcinoma of the human stomach was cultured in diffusion chambers, growth of epithelial and connective-tissue cells was observed. Unlike the original tumor tissue in vitro when the connective-tissue cells did not incorporate thymidine-H3, during growth in diffusion chambers they were labeled. The fraction of cells with labeled nuclei 1 h after injection of thymidine-H3 in the zone of growth averaged 25.1%, much greater than the proportion of labeled cells determined after incubation of the original tumor tissue in vitro (6.6%). Under the conditions of culture, a rapidly proliferating subpopulation of cells with a mitotic cycle 14.8 h in duration was discovered.

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Authors and Affiliations

  1. Sector of Kinetics of Chemical and Biological Processes, Institute of Chemical Physics, Academy of Sciences of the USSR, Moscow

    T. V. Krutova, M. S. Aksyutina, L. P. Lipchina & D. B. Korman

Authors
  1. T. V. Krutova
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  2. M. S. Aksyutina
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  3. L. P. Lipchina
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  4. D. B. Korman
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Additional information

Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 79, No. 6, pp. 83–85, June, 1975.

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Krutova, T.V., Aksyutina, M.S., Lipchina, L.P. et al. Autoradiographic investigation of schirrous carcinoma of the human stomach by culture in diffusion chambers. Bull Exp Biol Med 79, 680–682 (1975). https://doi.org/10.1007/BF00786431

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  • DOI: https://doi.org/10.1007/BF00786431

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