Abstract
A spectrofluorometric assay system for tryptophan oxygenase was used to compare dosage compensation properties and ontogenic expression of suppressed, “transformed,” and wild-type vermilion flies. The results indicate that, although different stocks showed different levels of oxygenase activity, all showed dosage compensation properties. The ontogenic expression of tryptophan oxygenase was observed to be different in the various genotypes. Whereas suppressed vermilion resembled wild type in its pattern, the ontogenic profiles of “transformed” flies were different.
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References
Avery, O. T., MacLeod, C. M., and McCarty, M. (1944). Studies on the chemical nature of the substance inducing transformation of pneumococcal types. J. Exp. Med. 79137.
Baglioni, C. (1960). Genetic control of tryptophan pyrrolase in Drosophila melanogaster and D. virilis. Heredity 1587.
Baillie, D. L., and Chovnick, A. (1971). Studies on the genetic control of tryptophan pyrrolase in Drosophila melanogaster. Mol. Gen. Genet. 112341.
Bowman, J. T., and Simmons, J. R. (1973). Gene modulation in Drosophila: Dosage compensation of Pgd + and Zw + genes. Biochem. Genet. 10319.
Camfield, R. G., and Suzuki, D. T. (1973). A temperature-sensitive vermilion mutant (v ts) in Drosophila melanogaster. Genetics Suppl. 74:s36 (abst.).
Casciano, D. A., and Gaertner, F. H. (1973). A specific and sensitive fluorometric assay for tryptophan oxygenase. Arch. Biochem. Biophys. 156658.
Ellgaard, E. G., and Brosseau, G. E., Jr. (1968). Puff forming ability as a function of chromosomal position in Drosophila melanogaster. Genetics 62337.
Elston, R. C., and Glassman, E. (1967) An approach to the problem of whether clustering of functionally related genes occurs in higher organisms. Genet. Res. 9147.
Fox, A. S., and Valencia, J. I. (1973) Cytological detection of structural changes at the sites of DNA-induced transformations in the polytene chromosomes of Drosophila. Genetics Suppl. 74:s83 (abst.).
Fox, A. S., Yoon, S. B., Duggleby, W. F., and Gelbart, W. M. (1971a). Genetic transformation in Drosophila. In Ledoux, L. (ed.), Informative Molecules in Biological Systems, North-Holland, Amsterdam, p. 313.
Fox, A. S., Yoon, S. B., and Gelbart, W. M. (1971b). DNA-induced transformation in Drosophila: Genetic analysis of transformed stocks. Proc. Natl. Acad. Sci. 68342.
Kaufman, S. (1962). Studies on tryptophan pyrrolase in Drosophila melanogaster. Genetics 45807.
Ledoux, L., ed. (1971). Informative Molecules in Biological Systems, North-Holland, Amsterdam, 466 pp.
Ledoux, L., Huart, R., and Jacobs, M. (1974). DNA-mediated genetic correction of thiamineless Arabidopsis thaliana. Nature 24917.
Lifschytz, E., and Lindsley, D. L. (1972). The role of X-chromosome inactivation during spermatogenesis. Proc. Natl. Acad. Sci. 69182.
Lindsley, D. L. (1965). Chromosome function at the supragenic level. Natl. Cancer Inst. Monogr. 18275.
Lindsley, D. L., and Grell, E. H. (1968). Genetic Variations of Drosophila melanogaster, Carnegie Institute of Washington Publication No. 627, Washington, D.C.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951). Protein measurements with the Folin phenol reagent. J. Biol. Chem. 193265.
Lucchesi, J. C. (1973). Dosage compensation in Drosophila. Ann. Rev. Genet. 7225.
Marzluf, G. A. (1965). Enzymatic studies with the suppressor of vermilion of Drosophila melanogaster. Genetics 52503.
Mishra, N. C., and Tatum, E. L. (1973). Non-Mendelian inheritance of DNA-induced inositol independence in Neurospora. Proc. Natl. Acad. Sci. 703875.
Nawa, S., and Yamada, M.-A. (1968). Hereditary change in Ephestia after treatment with DNA. Genetics 58573.
Novitski, E., and Brosseau, G. E., Jr. (1964). The continuity of the chromosome. Genetics 50273 (abst.).
Seecof, R. L., Kaplan, W. D., and Futch, D. G. (1969). Dosage compensation for enzyme activities in Drosophila melanogaster. Proc. Natl. Acad. Sci. 62528.
Tartof, K. D. (1969). Interacting gene systems. I. The regulation of tryptophan pyrrolase by the vermilion-suppressor of vermilion system in Drosophila. Genetics 62781.
Tobler, J. (1971). The effect of chromosomal position on dosage compensation and ontogenic expression of the v + gene in Drosophila melanogaster. Ph.D. thesis, Utah State University, Logan, Utah.
Tobler, J., Bowman, J. T., and Simmons, J. R. (1968). Enzymatic study of a position effect in Drosophila melanogaster. Genetics 60231 (abst.).
Tobler, J., Bowman, J. T., and Simmons, J. R. (1971a). Gene modulation in Drosophila: Dosage compensation and relocated v + genes. Biochem. Genet. 5111.
Tobler, J., Bowman, J. T., and Simmons, J. R. (1971b). A change in the ontogenic expression of a gene resulting from a translocation in Drosophila melanogaster. Genetics Suppl. 68:s67 (abst.).
Twardzik, D. R., Grell, E. H., and Jacobson, K. B. (1971). Mechanism of suppression in Drosophila: A change in tyrosine transfer RNA. J. Mol. Biol. 57231.
Yoon, S. B., and Fox, A. S. (1965). Permeability of premature eggs from Drosophila collected with the “ovitron.” Nature 206910.
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This research was sponsored by the U.S. Atomic Energy Commission under contract with the Union Carbide Corporation. The author is a Postdoctoral Investigator supported by USPHS Fellowship No. 1-FO2-GM53673-01.
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Tobler, J.E. Dosage compensation and ontogenic expression of suppressed and transformed Vermilion flies in Drosophila . Biochem Genet 13, 29–43 (1975). https://doi.org/10.1007/BF00486004
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DOI: https://doi.org/10.1007/BF00486004