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Genetic variation, inheritance, and quaternary structure of malic enzyme in brook trout (Salvelinus fontinalis)

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Abstract

Electrophoretic variation is described for malic enzyme (ME) for the first time in brook trout (Salvelinus fontinalis). Since the quaternary structure of ME was not clear from examination of banding patterns in brook trout alone, ME phenotypes in rainbow trout (Salmo gairdneri) × brook trout hybrids as well as in esocid species demonstrated that ME is tetrameric. A model of two duplicated loci is proposed to account for the observed variation. One locus (ME-2) is fixed and one locus (ME-1) is variable with three electrophoretically distinct alleles; the protein products of ME-1 are reduced in activity relative to the protein products of ME-2. Joint segregation was examined between ME-1 and ten other biochemical loci in brook trout, and between ME-1, ME-2, and nine other biochemical loci in a splake—lake trout (Salvelinus namaycush) × brook trout hybrid—backcross. All pairwise examinations showed random assortment except ME-2 with an isocitrate dehydrogenase locus (IDH-3), which showed complete linkage in the splake backcross. This may be due to a chromosomal aberration.

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Author notes

  1. Mark Stoneking

    Present address: Department of Genetics, University of Wisconsin, 53706, Madison, Wisconsin

Authors and Affiliations

  1. Department of Biology, The Pennsylvania State University, 16802, University Park, Pennsylvania

    Mark Stoneking, Bernie May & James E. Wright Jr.

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  1. Mark Stoneking
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  2. Bernie May
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  3. James E. Wright Jr.
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Additional information

Authorized for publication as Paper No. 5599 in the Journal Series of The Pennsylvania Agricultural Experiment Station, University Park, Pennsylvania, in cooperation with the Benner Spring Fish Research Station, The Pennsylvania Fish Commission, Bellefonte, Pennsylvania. M.S. was supported by an NSF Graduate Fellowship.

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Stoneking, M., May, B. & Wright, J.E. Genetic variation, inheritance, and quaternary structure of malic enzyme in brook trout (Salvelinus fontinalis) . Biochem Genet 17, 599–619 (1979). https://doi.org/10.1007/BF00502121

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