Abstract
An electrophoretic procedure for separating the molecular forms of catechol-O-methyltransferase in cellulose acetate gel is described; the zones of enzyme activity were revealed by autoradiography. The electrophoretic patterns of the enzyme in several tissues and cell lines derived from four different species are presented.
Similar content being viewed by others
References
Assicot, M., and Bohuon, C. (1971). Presence of two distinct catechol-O-methyltransferase activities in red blood cells. Biochimie 53871.
Axelrod, J. (1977). In Salvatore F., Borek, E., Zappia, V., Williams-Ashman, H. G., and Schlenk, F. (eds.), The Biochemistry of Adenosylmethione Columbia University Press, New York, pp. 539–553.
Axelrod, J., and Tomchick, R. (1958). Enzymatic O-methylation of epinephrine and other catechols. J. Biol. Chem. 233702.
Axelrod, J., and Vesell, E. S. (1970). Heterogeneity of N- and O-methyltransferases. Mol. Pharmacol. 678.
Beutler, E., Guinto, E., and Kuhl, W. (1973). Variability of β-galactosidase A and B in different tissues of man. Am. J. Hum. Genet. 2542.
Borchardt, R. T., Cheng, C.-F., Cooke, P. H., and Creveling, C. R. (1974). The purification and kinetic properties of liver microsomal-catechol-O-methyltransferase. Life Sci. 141089.
Brahe, C., Serra, A., and Crosti, N. (1981). Catechol-O-methyltransferase activity in Down's syndrome. ATTI Ass. Genet. Ital. 2775.
Floderus, Y., Ross, S. B., and Wetterberg, L. (1981). Erythrocyte catechol-O-methyltransferase activity in a Swedish population. Clin. Genet. 19389.
Frère, J. M., and Verly, W. G. (1971). Catechol-O-methyltransferase. The para- and meta-O-methylations of noradrenaline. Biochim. Biophys. Acta 23573.
Goldin, L. R., Gershon, E. S., Lake, C. R., Murphy, D. L., McGinniss, M., and Sparkes, R. S. (1982). Segregation and linkage studies of plasma dopamine-beta-hydroxylase (DBH), erythrocyte catechol-O-methyltransferase (COMT), and platelet monoamine oxidase (MAO): Possible linkage between the ABO locus and a gene controlling DBH activity. Am. J. Hum. Genet. 34250.
Lowry, O. H., Rosebrough, N. I., Farr, A. L., and Randall, R. J. (1951). Protein measurement with the Folin Phenol reagent. J. Biol. Chem. 193265.
Meera Khan, P. (1971). Enzyme electrophoresis on cellulose acetate gel: Zymogram patterns in man-mouse and man-Chinese hamster somatic cell hybrids. Arch. Biochem. Biophys. 145470.
Raymond, F. A., and Weinshilboum, R. M. (1975). Microassay of human erythrocyte catechol-O-methyltransferase: Removal of inhibitory calcium ion with chelating resin. Clin. Chim. Acta 58185.
Rock, G. D., Tong, J. H., and D'lorio, A. D. (1970). A comparison of brain and liver catechol-O-methyltransferase. Can. J. Biochem. 481326.
Shin, S., Meera Khan, P., and Cook, P. R. (1971). Characterization of hypoxanthine-guanine phosphoribosyl transferase in man-mouse somatic cell hybrids by an improved electrophoretic method. Biochem. Genet. 591.
Spielman, R. S., and Weinshiboum, R. M. (1981). Genetics of red cell COMT activity: Analysis of thermal stability and family data. Am. J. Med. Genet. 10279.
Tong, J. H., and D'lorio, A. (1977). Solubilization and partial purification of particulate catechol-O-methyltransferase from rat liver. Can. J. Biochem. 551108.
Veser, J., Geywitz, P., and Thomas h. (1978). Fluorometrischer Nachweis der Catechol-O-Methyltransferase-Aktivität nach Electrophorese im Polyacrylamid-Gel. J. Chromatogr. 150562.
Weinshilboum, R. M., Raymond, F. A., and Frohnauer, M. (1979). Monogenic inheritance of catechol-O-methyltransferase activity in the rat—Biochemical and genetic studies. Biochem. Pharmacol. 281239.
White, H. L., and Wu, J. C. (1975). Properties of catechol-O-methyltransferases from brain and liver of rat and human. Biochem. J. 145135.
Author information
Authors and Affiliations
Additional information
This investigation was supported by Grant 500.6/Ric. 70/1981 from the Italian Ministry of Health and by the Italian Ministry of Education. We are grateful to Dr. M. Castagnola for useful advice and help with thin-layer chromatography.
Rights and permissions
About this article
Cite this article
Brahe, C., Crosti, N., Khan, P.M. et al. Catechol-O-methyltransferase: A method for autoradiographic visualization of isozymes in Cellogel. Biochem Genet 22, 125–132 (1984). https://doi.org/10.1007/BF00499292
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00499292