Abstract
This study describes the biochemical characterization, genetic variation, and linkage of a codominantly inherited murine esterase, termed ES-18. The enzyme was identified by isoelectric focusing of supernatants obtained after centrifugation of tissue homogenates and subsequent staining for esterase using either α-naphthyl acetate or 4-methylumbelliferyl elaidate as substrate. ES-18 exhibited an organ-specific variation of the intensity pattern of bands as seen in kidney, spleen, and macrophages, respectively. Its activity was highly sensitive to inhibition by 1 mmol · liter−1 p-chloromercuriphenyl-sulfonate but was resistant to bis-p-nitrophenyl phosphate. Four allozymes could be distinguished in kidney supernatants obtained from the inbred strains C57BL/10Sn (ES-18A), MOLF/Ei (ES-18B), WLL/BrA (ES-18C), and CAST/Ei (ES-18D). The enzyme is shown to be controlled by a structural locus,Es-18, which resides on chromosome 19. The gene orderLy-1—Got-1—4.7±1.6—Es-18 is suggested.
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This work was supported by the Deutsche Forschungsgemeinschaft (De 315/2). This is Communication No. 64 of a research program devoted to the cellular distribution, genetics, and regulation of nonspecific esterases.
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von Deimling, O.H., Gaa, A. & Simon, M.M. Esterase-18 (ES-18) of the house mouse (Mus musculus): Biochemical characterization and genetics of an allozyme system linked to chromosome 19. Biochem Genet 26, 617–629 (1988). https://doi.org/10.1007/PL00020501
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DOI: https://doi.org/10.1007/PL00020501