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Lymph node metastases versus DNA ploidy as prognostic factors for invasive ductal carcinoma of the breast

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Abstract

We evaluated the relationship between the DNA ploidy status and other variable prognostic factors, especially regional lymph node metastases, in 121 patients with invasive ductal carcinoma of breast, together with the value of these factors in estimating the prognosis of breast cancer. The ploidy status was diploid in 40% of the patients, and aneuploid in 60%. A significantly higher incidence of aneuploidy was found in patients with more than 4 positive axillary lymph nodes, positive internal mammary lymph nodes, or clinical stage 3 of malignancy. In a univariate study, overall survival and disease-free survival were significantly correlated with axillary and internal mammary lymph node metastases, tumor size, and clinical stage of malignancy. The disease-free survival rates for the diploid group tended to be somewhat higher than those for the aneuploid group of patients without axillary lymph node metastases. In the multivariate analysis, however, only axillary lymph node metastasis was significantly correlated with overall survival and disease-free survival. There was also a trend for the internal mammary lymph node metastases to be correlated with survival. As the DNA ploidy status was closely correlated with the axillary and internal mammary lymph node metastases, it did not appear to be an independent prognostic factor in this small series.

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References

  1. Nemoto T, Vana J, Bedwani R, Baker H, McGregor F, Murphy G: Management and survival of female breast cancer: results of a national survey by the American College of Surgeons. Cancer 45: 2917–2924, 1980

    PubMed  Google Scholar 

  2. Veronesi U, Cascinelli N, Greco M, Bufalino R, Morabito A, Galluzzo D, Conti R, Lellis RD, Donne VD, Piotti P, Sacchini V, Clemente C, Salvadori B: Prognosis of breast cancer patients after mastectomy and dissection of internal mammary nodes. Ann Surg 202: 702–707, 1985

    PubMed  Google Scholar 

  3. National Cancer Institute: Clinical alert from the National Cancer Institute, May 16, 1988. Breast Cancer Res Treat 12: 3–5, 1988

    Google Scholar 

  4. McGuire WL, Dressler LG: Emerging impact of flow cytometry in predicting recurrence and survival in breast cancer patients. JNCI 75: 405–410, 1985

    PubMed  Google Scholar 

  5. Kute TE, Muss HB, Anderson D, Crumb K, Miller B, Burns D, Dube LA: Relationship of steroid receptor, cell kinetics, and clinical status in patients with breast cancer. Cancer Res 41: 3524–3529, 1981

    PubMed  Google Scholar 

  6. Cornelisse CJ, van de Velde CJH, Caspers RJC, Moolenaar AJ, Hermans J: DNA ploidy and survival in breast cancer patients. Cytometry 8: 225–234, 1987

    PubMed  Google Scholar 

  7. Kallioniemi OP, Blanco G, Alavaikko Met al. Tumour DNA ploidy as an independent prognostic factor in breast cancer. Br J Cancer 56: 637–642, 1987

    PubMed  Google Scholar 

  8. Hedley DW, Rugg CA, Alun BP, Taylor IW: Influence of cellular DNA content on disease-free survival of stage II breast cancer patients. Cancer Res 44: 5395–5398, 1984

    PubMed  Google Scholar 

  9. Fossa SD, Thorud E, Shoaib MC, Pettersen EO, Hoie J, Scott Knudsen O: DNA flow cytometry in primary breast carcinoma. Acta Path Microbiol Immunol Scand Sect A 92: 475–480, 1984

    Google Scholar 

  10. Jakobsen A, Skovgaard-Poulsen H, Lindegaard Madsen E, Ellebaeck Petersen S, Sommer Hansen H: Ploidy level of human breast carcinoma. Acta Radiol Oncol 23: 103–107, 1984

    PubMed  Google Scholar 

  11. Uyterlinde AM, Schipper NW, Baak JPA: Comparison of extent of disease and morphometric and DNA flow cytometric prognostic factors in invasive ductal breast cancer. J Clin Pathol 40: 1432–1436, 1987

    PubMed  Google Scholar 

  12. Deshpande N, Mitchell I, Allen D, Morris R: Deoxyribonucleic acid (DNA) content of carcinomas and prognosis in human breast cancer. Int J Cancer 32: 693–696, 1983

    PubMed  Google Scholar 

  13. Ewers SB, Langstrom E, Baldetorp B, Killander D: Flowcytometric DNA analysis in primary breast carcinomas and clinicopathological correlations. Cytometry 5: 408–419, 1984

    PubMed  Google Scholar 

  14. Moran RE, Black M, Alpert L, Strauss MJ: Correlation of cell-cycle kinetics, hormone receptors, histopathology, and nodal status in human breast cancer. Cancer 54: 1586–1590, 1984

    PubMed  Google Scholar 

  15. World Health Organization. The World Health Organization histological typing of breast tumors, second edition. Am J Clin Pathol 78: 806–816, 1982

    Google Scholar 

  16. Schutte B, Reynders MMJ, Bosman FT, Blijham GH: Flow cytometric determination of DNA ploidy level in nuclei isolated from paraffin-embedded tissue. Cytometry 6: 26–30, 1985

    PubMed  Google Scholar 

  17. Hedley DW, Friedlander ML, Taylor IW, Rugg CA, Musgrove EA: Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry. J Histochem Cytochem 31: 1333–1335, 1983

    PubMed  Google Scholar 

  18. Vindelov LL, Christensen IJ, Nissen NI: A detergent trypsin method for the preparation of nuclei for flow cytometric DNA analysis. Cytometry 3: 323–327, 1983

    PubMed  Google Scholar 

  19. Japan Breast Cancer Society: General rule for clinical and pathological record of breast cancer, 9th edition. Kinbara Co, Tokyo, 1988

    Google Scholar 

  20. Consensus Conference: Adjuvant chemotherapy for breast cancer. JAMA 254: 3461, 1985

    Google Scholar 

  21. McGuire W: Adjuvant therapy of node-negative breast cancer. N Engl J Med 8: 525–527, 1989

    Google Scholar 

  22. De Vita V: Breast cancer therapy: exercising all our options. N Engl J Med 8: 527–529, 1989

    Google Scholar 

  23. Beerman H, Kluin PM, Hermans J, van de Velde CJH, Cornelisse CJ: Prognostic significance of DNA-ploidy in a series of 690 primary breast cancer patients. Int J Cancer 45: 34–39, 1990

    PubMed  Google Scholar 

  24. Fisher B: The revolution in breast cancer surgery: science or anecdotalism? World J Surg 9: 655–666, 1985

    Article  PubMed  Google Scholar 

  25. Lewis WE: Prognostic significance of flow cytometric DNA analysis in node-negative breast cancer patients. Cancer 65: 2315–2320, 1990

    PubMed  Google Scholar 

  26. Winchester DJ, Duba RB, August CZ, Goldschmidt RA, Wruck DM, Rademaker AW, Winchester DP, Merkel DE: The importance of DNA flow cytometry in node-negative breast cancer. Arch Surg 125: 886–889, 1990

    PubMed  Google Scholar 

  27. Clark GM, Dressler LG, Owens MA, Pounds G, Oldaker T, McGuire WL: Prediction of relapse or survival in patients with node-negative breast cancer by DNA flow cytometry. N Engl J Med 320: 627–633, 1989

    PubMed  Google Scholar 

  28. Ewers SB, Baldetorp B, Killander D, Langstrom E: Flow cytometry DNA ploidy and number of cell populations in the primary breast cancer and their correlation to the prognosis. Acta Oncologica 28: 913–918, 1989

    PubMed  Google Scholar 

  29. Keyhani-Rofagha S, O'Toole RV, Farrar WB, Sickle-Santanello B, DeCenzo J, Young D: Is DNA ploidy an independent prognostic indicator in infiltrative node-negative breast adenocarcinoma? Cancer 65: 1577–1582, 1990

    PubMed  Google Scholar 

  30. Sigurdsson H, Baldetorp B, Borg A, Dalberg M, Ferno M, Killander D, Olsson H: Indicators of prognosis in nodenegative breast cancer. N Engl J Med 322: 1045–1053, 1990

    PubMed  Google Scholar 

  31. Wynder EL, Kajitani T, Kuno K, Lucas JC, Palo AD, Farro JA: Comparison of survival rates between American and Japanese patients with breast cancer. Surg Gynecol Obstet 117: 196–200, 1963

    PubMed  Google Scholar 

  32. Nemoto T, Tominaga T, Chamberlain A, Iwasa Z, Koyama H, Hama H, Bross I, Dao T: Differences in breast cancer between Japan and the United States. JNCI 58: 193–197, 1977

    PubMed  Google Scholar 

  33. Morrison AS, Black MM, Lowe CR, MacMahon B, Yuasa S: Some international differences in histology and survival in breast cancer. Int J Cancer 11: 261–267, 1973

    PubMed  Google Scholar 

  34. Sakamoto G, Sugano H, Hartmann WH: Comparative clinico-pathological study of breast cancer among Japanese and American females. Jap J Cancer Clin 25 (3): 161–170, 1979

    Google Scholar 

  35. Koike H: The impact of DNA ploidy and proliferative index on survival of breast cancer patients. Mie Medical Journal 38 (2): 111–124, 1988

    Google Scholar 

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Authors and Affiliations

  1. The Operation Center, Kanazawa University Hospital, Takara-machi, 13-1, 920, Kanazawa, Japan

    Masakuni Noguchi

  2. Department of Surgery (II), Kanazawa University, Japan

    Masakuni Noguchi, Takao Taniya, Nagayoshi Ohta, Naohiro Koyasaki & Itsuo Miyazaki

  3. Pathology Section, Kanazawa University Hospital, Japan

    Yuji Mizukami

  4. School of Medicine, Kanazawa University, Japan

    Yuji Mizukami

Authors
  1. Masakuni Noguchi
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  2. Takao Taniya
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  3. Nagayoshi Ohta
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  4. Naohiro Koyasaki
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  5. Itsuo Miyazaki
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  6. Yuji Mizukami
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Noguchi, M., Taniya, T., Ohta, N. et al. Lymph node metastases versus DNA ploidy as prognostic factors for invasive ductal carcinoma of the breast. Breast Cancer Res Tr 19, 23–31 (1991). https://doi.org/10.1007/BF01975201

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