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Functional analysis of peripheral blood lymphocytes isolated from patients with chronic hepatitis type B

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Abstract

Cell-mediated immunity, evaluated by lymphocyte proliferation and expression of the activation antigen interleukin-2 receptor in response to mitogens such as phytohemagglutinin and concanavalin-A, has been reported to be defective in chronic hepatitis B virus carriers. However, no definite conclusion on the functional state of T cells from these patients can be drawn. In the present study, we have investigated the expression of a wide set of lymphoid activation molecules as well as the proliferative response of peripheral blood lymphocytes isolated from patients with chronic hepatitis type B afterin vitro stimulation with monoclonal antibodies to both the T-cell receptor-CD3 complex and the CD2 molecule, which are the two main T-cell activation pathways. Our findings show that peripheral T lymphocytes from patients with chronic hepatitis type B express the activation antigens 4F2 molecule, interleukin-2 receptor, and activation inducer molecule (AIM) antigen, and proliferate normally after specific stimulation through either the T-cell receptor-CD3 complex or the CD2 molecule. These results suggest that the peripheral blood T cells of patients with chronic hepatitis B are fully operative and functionally competentin vitro.

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Authors and Affiliations

  1. Liver Unit Hospital de la Princesa, Universidad Autónoma de Madrid, Diego de León, No. 62, 28006, Madrid, Spain

    Carmelo García-Monzón MD, Ricardo Moreno-Otero MD, Luisa García-Buey MD, Miguel López-Botet MD, Manuel O. De Ladázuri MD & Francisco Sánchez-Madrid PhD

  2. Section of Immunology, Hospital de la Princesa, Universidad Autónoma de Madrid, Spain

    Carmelo García-Monzón MD

Authors
  1. Carmelo García-Monzón MD
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  2. Ricardo Moreno-Otero MD
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  3. Luisa García-Buey MD
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  4. Miguel López-Botet MD
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  5. Manuel O. De Ladázuri MD
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  6. Francisco Sánchez-Madrid PhD
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Additional information

This work was supported in part by a grant from the INSALUD (FISS 89/0068).

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García-Monzón, C., Moreno-Otero, R., García-Buey, L. et al. Functional analysis of peripheral blood lymphocytes isolated from patients with chronic hepatitis type B. Digest Dis Sci 37, 73–78 (1992). https://doi.org/10.1007/BF01308345

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  • DOI: https://doi.org/10.1007/BF01308345

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