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Gastrin receptor genes are expressed in gastric parietal and enterochromaffin-like cells ofMastomys natalensis

  • Esophageal, Gastric, And Duodenal Disorders
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Abstract

Although gastric enterochromaffin-like (ECL) carcinoid tumors are known to develop in patients with long-standing hypergastrinemia, the expression of the gastrin receptor gene in ECL cells has not yet been demonstrated. Therefore, this study was designed to examine gastrin receptor gene expression in ECL cells.Mastomys gastric mucosal cells isolated by enzyme dispersion were separated into 10 fractions (F1–10) by centrifugal elutriation. Each fraction was examined histologically to determine whether they contained ECL and/or parietal cells and Northern blot analysis was used to confirm the presence of histidine decarboxylase and H+, K+-ATPase gene expression. ECL cells were found only in fractions 1 and 2, whereas parietal cells were detected in fractions 6–10. Gastrin receptor gene expression was demonstrated in both parietal cell-rich and ECL cell-rich fractions. In addition, the gastrin receptor cDNA sequences obtained from the two of the fractions (F1 and 8) were identical. These results suggest that gastrin receptor genes are expressed in ECL cells as well as in parietal cells and that these receptors are identical.

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Authors and Affiliations

  1. Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, 606, Kyoto, Japan

    Masakyo Asahara MD, Yoshikazu Kinoshita MD, Hirohisa Nakata MD, Yumi Matsushima MD, Yoko Naribayashi MD, Akira Nakamura MD, Toshimitsu Matsui MD, Kazuo Chihara MD, Jun Yamamoto PhD, Atsushi Ichikawa PhD &  Tsutomu Chiba MD

  2. Division of Gerontology, Department of Internal Medicine, Kobe University School of Medicine, Kusunoki-cho 7-5-2, Chuo-Ku, 650, Kobe, Japan

    Masakyo Asahara MD, Yoshikazu Kinoshita MD, Hirohisa Nakata MD, Yumi Matsushima MD, Yoko Naribayashi MD, Akira Nakamura MD, Toshimitsu Matsui MD, Kazuo Chihara MD, Jun Yamamoto PhD, Atsushi Ichikawa PhD &  Tsutomu Chiba MD

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  1. Masakyo Asahara MD
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  2. Yoshikazu Kinoshita MD
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  3. Hirohisa Nakata MD
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  4. Yumi Matsushima MD
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  5. Yoko Naribayashi MD
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  6. Akira Nakamura MD
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  7. Toshimitsu Matsui MD
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  8. Kazuo Chihara MD
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  9. Jun Yamamoto PhD
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  10. Atsushi Ichikawa PhD
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  11. Tsutomu Chiba MD
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Additional information

This study was supported by a grant-in-aid for scientific research from the Ministry of Education, Science, and Culture, and a grant from the Ministry of Health and Welfare, Japan.

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Asahara, M., Kinoshita, Y., Nakata, H. et al. Gastrin receptor genes are expressed in gastric parietal and enterochromaffin-like cells ofMastomys natalensis . Digest Dis Sci 39, 2149–2156 (1994). https://doi.org/10.1007/BF02090363

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  • DOI: https://doi.org/10.1007/BF02090363

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