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Effect of L-Lactic Acid, Short-Chain Fatty Acids, and pH in Cecal Infusate on Morphometric and Cell Kinetic Parameters of Rat Cecum

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Abstract

We studied the influences of cecal infusion ofNaCl, short-chain fatty acids (SCFA), and L-lactic acidat pH 5.0 or 7.0 for seven days on morphometric and cellkinetic parameters of the rat cecum. SCFA increased relative weight of the mucosa andsubmucosa, crypt size, and mitotic index in the cecum.L-Lactic acid stimulated mitosis only at pH 5.0. Cryptsize correlated positively to epithelial proliferative activity only when NaCl or L-lactic acid wasinfused. SCFA should have changed the balance betweenproduction and loss of the cecal epithelial cells. Theinfusate pH by itself had no effect, but modified the effects of SCFA and L-lactic acid indifferent ways. Crypt size correlated positively to thelogarithm of daily proton load of infusates. The aboveresults indicate that epithelial cell proliferation in the cecum is influenced by both SCFA andL-lactic acid, although differently, and by protonload.

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Authors
  1. Hirofumi Ichikawa
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  2. Takashi Sakata
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Ichikawa, H., Sakata, T. Effect of L-Lactic Acid, Short-Chain Fatty Acids, and pH in Cecal Infusate on Morphometric and Cell Kinetic Parameters of Rat Cecum. Dig Dis Sci 42, 1598–1610 (1997). https://doi.org/10.1023/A:1018884625737

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