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An Apparently Novel Protein of Human Leukocytes, Reactive with an Antibody to Protein Kinase C-γ, Is Rapidly Modified upon Cell Activation: Initial Characterization in Neutrophils and Their Cytoplasts

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Abstract

On immunoblots of human neutrophil cytoplasts (U-CYT), a previously undescribed 97kDa protein was revealed by intense and selective reaction with an antibody that was initially raised to recognize PKC-γ. Denoted “γ-rp” for gamma-related protein, this acidic cytosolic protein somewhat resembled the classic forms of PKC in several biochemical respects. Appearing as a doublet on low-percentage SDS-PAGE gels, both its mobility and staining pattern were rapidly altered by treatment of U-CYT with either phorbol ester or chemotactic peptide. Whole neutrophil γ-rp was detectable only after TCA precipitation of intact cells. It was also detectable in human platelets, lymphocytes, and neutrophil-like differentiated HL60 cells, but not in fibroblasts, erythrocytes, monocytes, or monocyte-like differentiated HL60 cells. Our data suggest that γ-rp merits further study as a potential participant in cellular activation, and as a possible structural or functional relative of PKC.

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  1. Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut, 06520-8031

    Joan I. Smallwood & Stephen E. Malawista

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  1. Joan I. Smallwood
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Smallwood, J.I., Malawista, S.E. An Apparently Novel Protein of Human Leukocytes, Reactive with an Antibody to Protein Kinase C-γ, Is Rapidly Modified upon Cell Activation: Initial Characterization in Neutrophils and Their Cytoplasts. Inflammation 22, 1–28 (1998). https://doi.org/10.1023/A:1022390406225

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