Skip to main content
SpringerLink
Log in

Plasma Substance-P and Substance-K and Gonadal Steroids in Relation to the Gonadotropin Surge in Normal Human Reproductive Cycles

  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose: This study wax designed to examine changes in peripheral plasma substance-P and -K levels, their association with follicle-stimulating hormone and luteinizing hormone release in normal reproductive cycles in humans, and their correlation with plasma estradiol and progesterone.

Methods: Fourteen healthy, normally menstruating women underwent daily blood sampling (cycle day 4, 4–14 days) for measurement of estradiol, progesterone, luteinizing hormone, and follicle-stimulating hormone, substances-P and -K, and daily transvaginal ultrasounds assessing follicular growth and documentation of ovulation.

Results: Estradiol peaked on day 13, luteinizing hormone and follicle-stimulating hormone peaked on day 14, and progesterone began an exponential increase on about day 13.

Conclusions: In contrast to other experimental designs using in vitro or in vivo rat or monkey tissue, peripheral levels of substances-P (P = 0.8391) and -K (P = 0.3205) reflected no modulation related to midcycle gonadotropin release in cycling woman.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. Brown ER, Harlan RE, Kruase JE: Gonadal steroid regulation of substance P (SP) and SP-encoding messenger ribonucleic acids in the rat anterior pituitary and hypothalamus. Endocrinology 1990;126:330-340

    Google Scholar 

  2. Kerdelhué B, Tartar A, Lenoir V, El Abed A, Hublau P, Millar RP: Binding studies of Substance P anterior pituitary binding sites: changes in Substance P binding sites during the rat estrus cycle. Regul Peptides 1985;0:133-144

    Google Scholar 

  3. Maggio JE: Tachykinins. Annu Rev Neurosci 1988;11:13-28

    Google Scholar 

  4. Wormald PJ, Millar RP, Kerdelhué B: Substance P receptors in human pituitary: A potential inhibitor of luteinizing hormone secretion. J Clin Endocrinol Metab 1989;69:612-615

    Google Scholar 

  5. Debeljuk L, Ghosh P, Bartke A: Neurokinin A levels in the hypothalamus of rats and mice: effects of castration, gonadal steroids and expression of heterologous growth hormone genes. Brain Res Bull 1990;25:717-721

    Google Scholar 

  6. Debeljuk L, Bartke A: Immunoreactive substance P and neurokinin A in the hypothalamus and anterior pituitary gland of Siberian and Syrian hamsters and of rats. J Reprod Fertil 1994;101:427-444

    Google Scholar 

  7. Tsuruo Y, Hisano S, Okamura Y, Tsukamoto N, Diakoku S: Hypothalamic substance P-containing neurons. Sex dependent topographic differences and ultrastructural transformation associated with stages of the estrous cycle. Brain Res 1984;305:331-341

    Google Scholar 

  8. Cheramy A, Michelot R, Leviel V, Nieoullon A, Glowinski J, Kerdelhué B: Effect of the immunoneutralisation of Substance P in the cat substancia nigra on the release of dopamine from dendrites and terminals of dopaminergic neurons. Brain Res 1978;155:404-408

    Google Scholar 

  9. Makara GB, Kakucska I, Lenoir V, Kerdelhué B, Palkovits M: A substance P containing hypothalamic neuronal system projects to the median eminence. Brain Res 1986;374:399-401

    Google Scholar 

  10. Morel G, Chayvialle JA, Kerdelhué B, Dubois PM: Ultrastructural evidence for endogenous substance-P like immunoreactivity in the rat pituitary gland. Neuroendocrinology 1982;35:86-92

    Google Scholar 

  11. Sahu A, Kalra SP: Effects of tachykinins of luteinizing hormone release in female rats: Potent inhibitory action of neuropeptide K. Endocrinology 1992;130:1571-1577

    Google Scholar 

  12. Kerdelhué B, Valens M, Langlois Y: Substance P inhibits the secretion of gonadotropin in female rats. Proceedings 60th Annual Meeting, Endocrine Society, Miami, FL. 1978, abstract 186, p 167

  13. Parnet P, Lenoir V, Palkovits M, Kerdelhué B: Estrous cycle variations in gonadotropin-releasing hormone, substance P and β-endorphin contents in the median eminence, the arcuate nucleus and the medial preoptic nucleus in the rat: a detailed analysis of proestrus changes. J Neuroendocrinol 1990;2:291-296.

    Google Scholar 

  14. Duval P, Lenoir V, Moussaoui S, Garret C, Kerdelhué B: Substance P and neurokinin A variations throughout the rat estrous cycle; comparison with ovariectomized and male rats: I. Plasma, hypothalamus, anterior and posterior pituitary. J Neurosci Res 1996 (in press)

  15. Battman T, Parsadaniantz SM, Jeanjean B, Kerdelhué B: Invivo inhibition of the preovulatory LH surge by substance P and in-vitro modulation of gonadotrophin-releasing hormone-induced LH release by substance P. oestradiol and progesterone in the female rat. J Endocrinol 1991;130:169-175

    Google Scholar 

  16. Kerdelhué B, Jeanjean B, Lenoir V, Jones G, Hodgen GD: Progesterone induces the release of hypothalamic substance P by a direct time-dependent mechanism in a primate perifusion system. Proceedings, 70th Annual Endocrine Society Meeting, 1988 (abstract)

  17. Kerdelhué B, Jones GS, Gordon K, Seltman H, Lenoir V, Williams RF, Hodgen GD: Effects of Substance P and progesterone on the pituitary during the estradiol-induced LH surge in primates. Neuroendocrinology 1992;11:81-90

    Google Scholar 

  18. Kerdelhué B, Jones G, Gordon K, Seltman H, Lenoir V, Millar R, Williams R, Hodgen G: Hypothalamo-anterior pituitary gonadotropin-releasing hormone and substance P systems during the 17-β-estradiol-induced plasma luteinizing hormone surge in the ovariectomized female monkey. Endocrinology 1993;132:1151-1157

    Google Scholar 

  19. Kerdelhué B, Jones GS, Gordon K, Seltman H, Lenoir V, Williams, RF, Hodgen GD: The interaction between hypothalamic GnRH, β-endorphin and Substance P neuronal systems as key regulators of the LH surge: an hypothesis. Neuroendocr (Life Sci Adv) 1992;11:91-100

    Google Scholar 

  20. Wang CF, Lasley BL, Lein A, Yen SSC: The functional changes of the pituitary gonadotrophs during the menstrual cycle. J Clin Endocrinol Metab 1976;42:718-728

    Google Scholar 

  21. diZerega GS, Nixon WE, Hodgen GD: Intercycle serum FSH elevations: Significance in recruitment and selection of the dominant cycle. J Clin Endocrinol Metab 1980;50:1046-1048

    Google Scholar 

  22. Pickering AJMC, Fink G: Priming effect of luteinizing hormone releasing factor: in-vitro and in-vivo evidence consistent with its dependence upon protein and RNA synthesis. J Endocrinol Metab 1976;69:373-379

    Google Scholar 

  23. Menon M, Peegel H, Katta V: Estradiol potentiation of gonadotrophin releasing hormone responsiveness in the anterior pituitary is mediated by an increase in gonadotrophin releasing hormone receptors. Am J Obstet Gynecol 1985;151:534-540

    Google Scholar 

  24. Kerdelhué B, Khar A, Nay D, Langlois Y, Bernardo T, Liwska J, Jutisz M: Inhibition in vitro par la substance P de l'excrétion des gonadotropines induite par le LH-RH a partir de cellules adenohypophysaires de rat en culture. CR Acad Sci (Paris) 1070;287:879-882

  25. Katz E: The luteinized unruptured follicle and other ovulatory dysfunctions. Fertil Steril 1988;50:839-89

    Google Scholar 

  26. Yen SSC, Tsai CC, Naftolin F, Vandenberg G, Ajabor L: Pulsatile patterns of gonadotropin release in subjects with and without ovarian function. J Clin Invest 1972;34:671

    Google Scholar 

  27. Elkind-Hirsch K, Ravnikar V, Tulchinsky D, Schiff I, Ryan KJ: Episodic secretory patterns of immunoreactive luteinizing hormone-releasing hormone (IR-LH-RH) in the systemic circulation of normal women throughout the menstrual cycle. Fertil Steril 1984;41:56-61

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. The Jones Institute for Reproductive Medicine, Department of Obstetrics and Gynecology, Eastern Virginia Medical School, Norfolk, Virginia

    D. L. Mohyi, P. Kolm, H. W. Jones Jr. & G. S. Jones

  2. Laboratoire de Neurendocrinologie, Université Rene Descartes, Paris, France

    D. L. Mohyi, B. Kerdelhué & V. Lenoir

  3. Fertility Center of California, Orange, California, 92868

    D. L. Mohyi

Authors
  1. D. L. Mohyi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. Kerdelhué
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. Lenoir
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. P. Kolm
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. H. W. Jones Jr.
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. G. S. Jones
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mohyi, D.L., Kerdelhué, B., Lenoir, V. et al. Plasma Substance-P and Substance-K and Gonadal Steroids in Relation to the Gonadotropin Surge in Normal Human Reproductive Cycles. J Assist Reprod Genet 15, 547–551 (1998). https://doi.org/10.1023/A:1022534204239

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1022534204239

Search

Navigation