Abstract
Six compounds were identified from gland extracts of the cotton bollworm, Heliothis armigera(Hubner): (Z)-11-hexadecenal (Z11-16:Ald), (Z)-9-hexa-decenal (Z9-16:Ald), hexadecanal, (Z)-11-hexadecenol (Z11-16:OH), (Z)-7-hexadecenal (Z7-16:Ald), and (Z)-9-tetradecenal (Z9-14:Ald). Each of the compounds that were identified was examined for its ability to elicit sexual responses from male moths in a flight tunnel. Males flew upwind to Z11-16:Ald alone, but greater levels of copulatory responses were evoked with the addition of 2.5% Z9-16:Ald to the Z11-16:Ald. Addition of hexadecanal to the binary mixture had no effect in raising the behavioral response of the males in the flight tunnel. The effect of Z7-16:Ald on male flight depended on the loading. The addition of 1% of this component to 2 mg of the binary mixture reduced levels of copulatory response, but the same addition (1 %) to 10 μg of the binary mixture increased copulatory response. The addition of 79-14:Ald or Z11-16:OH to the binary mixture reduced behavioral responses of males. High loadings of the binary mixture (200–2000 μg) were better than a low loading (10 μg) in eliciting response of males.
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Buser, H. R., Arn, H., Guerin, P., and Rauscher, S. (1983). Determination of double bond position in mono-unsaturated acetates by mass spectrometry of dimethyl disulfide adducts.Anal. Chem. 55: 818–822.
Dunkelblum, E., and Kehat, M. (1989). Female sex pheromone components ofHeliothis peltigera (Lepidoptere: Noctuidae): Chemical identification from gland extracts and male response.J. Chem. Ecol. 15: 2233–2245.
Dunkelblum, E., Gothilf, S., and Kehat, M., (1980). Identification of the sex pheromone of the cotton bollworm,Hetiothis armigera, in Israel.Phytoparasitica 8: 209–211.
Dunkelblum, E., Tan, S. H., and Silk, P. J. (1985). Double-bond location in monounsaturated fatty acids by dimethyl disulfide derivatization and mass spectrometry: Application to analysis of fatty acids in pheromone glands of four Lepidoptera.J. Chem. Ecol. 11: 265–277.
Gothilf, S., Kehat, M., Jacobson, M., and Galun, R. (1978a). Screening pheromone analogues by EAG technique for biological activity on males ofEarias insulana, Heliothis armigera andSpodoptera littoralis.Envir. Entomol. 7: 31–35.
Gothilf, S., Kehat, M., Jacobson, M., and Galun, R. (1978b). Sex attractants for maleHeliothis armigera (Hbn).Experientia 34: 853–854.
Gothilf, S., Kehat, M. Dunkelblum, E., and Jacobson, M. (1979). Efficacy of (Z)-11-hexadecenal and (Z)-11-tetradecenal as sex attractants forHeliothis armigera on two different dispensers.J. Econ. Entomol. 72: 718–720.
Kehat, M., Gothilf, S., Dunkelbum, E., and Greenberg, S. (1980). Field evaluation of female sex pheromone components of the cotton bollworm,Heliothis armigera. Entomol. Exp. Appl. 27: 188–193.
Kehat, M., Gothilf, S., Dunkelblum, E., and Greenberg, S. (1982). Sex pheromone traps as a means of improving control programs for the cotton bollworm,Heliothis armigera (Lepidoptera: Noctuidae).Envir. Entomol. 11: 727–729.
Leonhardt, B. A., and De Vilbiss, E. D. (1985). Separation and double-bond determination on nanogram quantities of aliphatic monounsaturated alcohols, aldehydes and carboxylic acid methyl esters.J. Chromatogr. 322: 484–490.
Miller, J. R., and Roelofs, W. L. (1978). Sustained-flight tunnel for measuring insect responses to wind-borne sex pheromones.J. Chem. Ecol. 4: 187–198.
Nesbitt, B. F., Beevor, P. S., Hall, D. R., and Lester, R. (1979). Female sex pheromone components of the cotton Bollworm,Heliothis armigera. J. Insect Physiol. 25: 535–541.
Nesbitt, B. F., Beevor, P. S., Hall, D. R., and Lester, R. (1980). (Z)-9-Hexadecenal: A minor component of the female sex pheromone ofHeliothis armigera.Entomol. Exp. Appl. 27: 306–308.
Piccardi, P., Capizzi, A., Cassani, G., Spinelli, P., Arsura, E., and Massardo, P. (1977). A sex pheromone component of the Old World bollworm,Heliothis armigera. J. Insect Physiol. 23: 1443–1445.
Pope, M. M., Gaston, L. K., and Baker, T. C. (1982). Composition, quantfication, and periodicity of sex pheromone gland volatiles from individualHeliothis virescens females.J. Chem. Ecol. 8: 1043–1055.
Pope, M. M., Gaston, L. K., and Baker, T. C. (1984). Composition, quantification, and periodicity of sex pheromone gland volatiles from individualHeliothis zea females.J. Insect Physiol. 30: 943–945.
Roelofs, W., and Bjostad, L. (1984). Biosynthesis of lepidopteran pheromones.Bioorg. Chem. 12: 279–298.
Rothschild, G. H. L. (1978). Attractants forHeliothis armigera and H. punctigera.J. Aust. Entomol. Soc. 17: 389–390.
Ryan, J. A. (1960). Significance tests for multiple comparison of proportions, variances, and other statistics.Psychol. Bull. 57: 318–328.
Shorey, H. H., and Hale, R. L. (1965). Mass-rearing of the larvae of nine Nocutid species on a simple artificial medium.J. Econ. Entomol. 58: 522–524.
Teal, P. E. A., and Tumlinson, J. H. (1987). The role of alcohols in pheromone biosynthesis by two noctuid moths that use acetate pheromone components.Arch. Insect Biochem. Physol. 4: 261–269.
Teal, P. E. A., Tumlinson, J. H., and Heath, R. R. (1986). Chemical and behavioral analyses of volatile sex pheromone components released by callingHeliothis virescens (F.) females (Lepidoptera: Noctuidae).J. Chem. Ecol. 12: 107–126.
Vetter, R. S., and Baker, T. C. (1984). Behavioral responses of maleHeliothis zea moths in sustained-flight tunnel to combinations of 4 compounds identified from female sex pheromone gland.J. Chem. Ecol. 10: 193–202.
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Contribution from the Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel. No. 2455-E, 1988 series.
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Kehat, M., Dunkelblum, E. Behavioral responses of maleHeliothis armigera (Lepidoptera: Noctuidae) moths in a flight tunnel to combinations of components identified from female sex pheromone glands. J Insect Behav 3, 75–83 (1990). https://doi.org/10.1007/BF01049196
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DOI: https://doi.org/10.1007/BF01049196