Abstract
Trichosanthin, a type I ribosome-inactivating protein with RNAN-glycosidase activity, forms a stable complex with nicotinamide adenine dinucleotide phosphate, a substrate analog. Difference UV spectroscopy, fluorescence spectroscopy, and31P NMR are used to identify the formation of the complex, followed by a crystal structure analysis carried out to elucidate the active-site structure of trichosanthin. The determination of germinal vesicle breakdown indicates that the complex does not, at least for abortion-inducing activity, result in competitive inhibition to the protein.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- TCS:
-
trichosanthin
- RIP:
-
ribosome-inactivating protein
- NADPH:
-
nicotinamide adenine dinucleotide phosphate
- AMP:
-
adenosine 5′-mondphosphate
- ATP:
-
adenosine 5′-triphosphate
- CMP:
-
cytidine 5′-monophosphate
- GTP:
-
guanosine 5′-triphosphate
- UMP:
-
uridine 5′-monophosphate
- NADH:
-
nicotinamide adenine dinucleotide
- GVBD:
-
germinal vesicle breakdown
- HIV:
-
human immunodeficiency virus
References
Barbieri, L., and Stirpe, F. (1982).Cancer Surv. 1, 489–520.
Dai, R.-X., Xu, G.-J., Lin, X.-Y., Liu, L.-Y., Shen, H.P., Zhou, X.-G., Gao, T., and Wang, Y.-H. (1993).Acta Biol. Exp. Sinica 26, 411–427.
Endo, Y., and Tsurugi, K. (1987).J. Biol. Chem. 262, 8128–8130.
Gao, B., Ma, X.-Q., Wang, Y.-P., Wu, S., Chen, S.-Z., and Dong, Y.-C. (1993).Science China B 23 (Chinese ed.), 273–282.
Katzin, B. J., Collins, E. J., and Robertus, J. D. (1991).Proteins 10, 251–259.
McGrath, M. S., Hwang, K. M., Caldwekk, S. E., Gaston, I. K.-C., Wu, P., Ng, V. L., Crowe, S., Danids, J., Marsh, J., Deunhart, T., Lekas, P. V., Vennari, J. C., Yeung, H.-W., and Lifson, J. P. (1989).Proc. Natl. Acad. Sci. USA 86, 2844–2848.
Monzingo, A. F., and Robertus, J. D. (1992).J. Mol. Biol. 227, 1136–1145.
Olsnes, S., and Pihl, A. (1982). InMolecular Action of Toxins and Viruses (Cohen, P., and Van Heyningen, S., eds.), Elsevier, Amsterdam, pp. 51–105.
Ren, J., Wang, Y., Dong, Y., and Stuart, D. (1994).Structure 2, 7–16.
Roberts, W. K., and Selitrennikoff, C. P. (1986).Biosci. Rep. 6, 19–29.
Stirpe, F., and Barbieri, L. (1986).FEBS Lett. 195, 1–8.
Wang, J. H., Wang, Y. P., and Tian, G. Y. (1985).Kexue Tongbao 30, 1396–1398.
Wang, Y., Quian, R.Q., Gu, Z.-W., Jin, S.-W., Zhang, L.-Q., Xia, Z.-X., Tian, G.-Y., and Ni, C.-Z. (1986).Pure Appl. Chem. 58, 789–798.
Xia, Z.-X., Zhang, L., Zhang, Z.-M., Wu, S., and Dong, Y.-C. (1993).Chin. J. Chem. 11, 280–288.
Xiong, J.-P., Xia, Z.-X., and Wang, Y. (1994).Nature Structural Biol. 1, 695–700.
Zhang, J.-S., and Liu, W.-Y. (1992).Nucleic Acids Res. 20, 1271–1275.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Xiong, JP., Xia, ZX. & Wang, Y. Identification of a stable complex of trichosanthin with nicotinamide adenine dinucleotide phosphate. J Protein Chem 14, 139–144 (1995). https://doi.org/10.1007/BF01980325
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF01980325