Abstract
The age related decrease in alpha1-adrenergic stimulated inositol 1,4,5 trisphosphate (IP3) production in parotid cells of aged rats can be partially restored by treatment with S-adenosylmethionine (SAM). This effect is completely blocked by S-adenosyl homocysteine (SAH) and occurs in association with an increase in the conversion of phosphatidylethanolamine to phosphatidylcholine and a decrease in membrane viscosity. In contrast, SAM treatment actually inhibits stimulated IP3 production in cells of young rats. The membrane viscosity of these cells is lower than that of those from aged rats. Although conversion of phosphatidylethanolamine to phosphatidylcholine is enhanced, no further decrease in membrane viscosity is elicited in young cell preparations. These findings suggest that age changes in the membrane environment may result in impaired alpha1-adrenergic signal transduction and that such alterations may be at least partially reversible by SAM treatment.
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References
Roth GS: Mechanisms of altered hormone/neurotransmitter action during aging. Ann Rev Gerontol Geriatrics 10: 132–146, 1990
Joseph JA, Roth GS: Hormonal regulation of motor behavior in senescence. J Gerontol 48: 51–55, 1993
Ito H, Baum BJ, Uchida T, Hoopes MT, Bodner L, Roth GS: Modulation of rat parotid alpha adrenergic responsiveness at a step subsequent to receptor activation. J Biol Chem 257: 9532–9538, 1982
Bodner L, Hoopes MT, Gee M, Ito H, Roth GS, Baum BJ: Multiple transduction mechanisms are likely involved in calcium mediated exocrine secretory events in rat parotid cells. J Biol Chem 258: 2774–2777, 1983
Miyamoto A, Villalobos-Molina R, Kowatch MA, Roth GS: Altered coupling of alpha1-adrenergic receptor-G-protein in rat parotid during aging. Am J Physiol 262: C1181-C1188, 1992
Wu D, Katz A, Lee CH, Simon MI: Activation of phospholipase C by alpha1-adrenergic receptors is mediated by the alpha subunits of Gq family. J Biol Chem 267: 25798–25802, 1992
Wu D, Lee CH, Rhee SG and Simon MI: Activation of phospholipase C by the alpha subunits of the Gq and G11 proteins in transfected Cos-7 cells. J. Biol. Chem. 267: 1811–1817, 1992
Putney JW Jr: Identification of cellular activation mechanisms associated with salivary secretion. Ann Rev Physiol 48: 75–88, 1986
Miyamoto A, Kowatch MA and Roth GS: Similar effects of saponin treatment and aging on coupling of alpha1-adrenergic receptor-G-protein. Exp Gerontol 28: 349–359, 1993
Kowatch MA, Roth GS: Effect of specific membrane perturbations on alpha,-adrenergic and muscarinic-cholinergic signal transduction in rat parotid cell aggregates. Life Sci 55: 2003–2010, 1994
Hirata F, Axelrod J: Enzymatic methylation of phosphatidyl-ethanolamine increases erythrocyte membrane fluidity. Nature 275: 219–220, 1978
Vance DE, deKruijff B: The possible functional significance of phosphatidyl ethanolamine methylation. Nature 288: 277–279, 1980
Fonlupt P, Benistant C, Rey C and Logarde M: S-adenosyl-1-methionine inhibits phosphoinositide metabolism in the rat brain synaptosomal suspensions. Mol Cell Biochem 124: 51–57, 1993
Folch J, Lees M, Sloane-Stanley GH: A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226: 497–509, 1957
Rouser G, Saikotos AN, Fleisher S: Quantitative analysis of phospholipids by thin layer chromatography and phosphorus analysis of spots. Lipids 1: 85–86, 1966
Kuhry JG, Fonteneau P, Duportail G, Maechling C, Laustriat G: TMA-DPH: A suitable flouorescence polarization probe for specific plasma membrane fluidity studies in intact living cells. Cell Biophysics 5: 129–140, 1983
Miyamoto A, Araiso T, Koyama T, Ohshika H: Adrenoceptor coupling mechanisms which regulate salivary secretion during aging. Life Sci 53: 1873–1878, 1993
Fonlupt P, Rey C, Pacheco H: Phosphatidylethanolamine methylation in membranes from rat cerebral cortex: effect of exogenous phospholipids and S-adenosylhomocysteine. Biochem Biophys Res Comm 100: 1720–1726, 1981
Lederer ED, Jacobs M, Hoffman JL, Harding GB, Robishaw JD, McLeish KR: Role of carboxylmethylation in chemoattractant receptor-stimulated G protein activation and functional responses. Biochem Biophys Res Commun 200: 1604–1614, 1994
Anson RM, Cutler R, Joseph JA, Yamagami K, Roth GS: The effects of aging on muscarinic receptor/G-protein coupling in the rat hippocampus and striatum. Brain Res 598: 302–306, 1992
DeKeyser J, DeBacker JP, Ebinger G and Vauquelin G: Coupling of D1 dopamine receptors to the guanine nucleotide binding protein Gs is deficient in Huntington's disease. Brain Res 496: 327–330, 1989
Smith CJ, Perry EK, Perry RH, Fairbavin AF, Birdsall NJM: Guanine nucleotide modulation of muscarinic cholinergic receptor binding in postmortem human brain-a preliminary study in Alzheimer's disease. Neurosci Lett 82: 227–232, 1987
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Kowatch, M.A., Kelly, J.F., Denisova, N.A. et al. Partial restoration of impaired alpha1-adrenergic responsiveness in parotid cells of aged rats by S-adenosylmethionine treatment. Mol Cell Biochem 148, 73–77 (1995). https://doi.org/10.1007/BF00929505
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DOI: https://doi.org/10.1007/BF00929505