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Occurrence of phenolsulfotransferase in primary glial culture cells of rat

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Abstract

Phenolsulfotransferase (PST) activity towards phenol and monoamines was determined in rat brain and in primary cultures of rat astrocytes. The pH requirement.K m values and the proportion of PST activity with respect to phenol and dopamine as substrates were similar between PST from the glial cells and the rat cortex. The enzyme activity increased with age in the brain of older animals, and also with increasing incubation time in the primary culture of astroglia. The specific PST activity of the astroglia appeared to be higher than that of the brain enzyme. In glial cultures treated with 0.25 mM dibutyryl cyclic AMP in the same culture conditions, PST activity is suppressed to about 25% of its untreated counterpart, even though dibutyryl cyclic AMP at concentrations of 1 mM only slightly inhibited PST activity in vitro.

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References

  1. Roth, J. A., andRiveti, J. 1982. Does sulfate conjugation contribute the metabolic inactivation of catecholamines in human? Biochem. Pharmacol. 31:3017–3021.

    Google Scholar 

  2. Foldes, A., andMeek, J. L. 1973. Rat brain phenolsulfotransferase—partial purification and some properties. Biochim. Biophys. Acta 327:365–374.

    Google Scholar 

  3. Foldes, A., andMeek, J. L. 1974. Occurence and localization of brain phenosulpho-transferase. J. Neurochem. 23:303–307.

    Google Scholar 

  4. Young, W. F., Okazaki, H., Law, JR., E. R., andWeinshilboum, R. M. 1984. Human brain phenolsulfotransferase: biochemical properties and regional localization. J. Neurochem. 43:706–715.

    Google Scholar 

  5. Rein, G., Glover, V., andSandler, M. 1984. Characterization of human brain phenolsulphotransfarase. J. Neurochem. 42:80–85.

    Google Scholar 

  6. Jansen, G. S. I. M., Vrensen, G. F. J. M., andVan Kampen, G. M. J. 1974. Intracellular localization of phenolsulfotransferase in rat brain. J. Neurochem. 23:329–335.

    Google Scholar 

  7. Hirosawa, H., andYoung, R. W. 1971. Autoradiographic analysis of sulfate metabolism in the cerebellum of the mouse. Brain Res. 30:295–309.

    Google Scholar 

  8. Michelot, R. J., Lesko, N., Stout, R. W., andCoward, J. K. 1977. Effects of S-adenosylhomocystein and S-tubercidinyl homocysteine on catecholamine methylation in neuroblastoma cells. Mol. Pharmacol. 13:368–373.

    Google Scholar 

  9. Moonen, G., Cam, Y., Sensenbrenner, M., andMandel, P. 1975. Variability of the effects of serum free medium, dibutyryl cyclic AMP or theophylline on the morphology of cultured new born rat astroblasts. Cell Tiss. Res. 167:365–372.

    Google Scholar 

  10. Schousboe, A., Nissen, C., Bock, E., Sapirstein, V. S., Juurlink, B. H. J., andHertz, L. 1980. Biochemical development of rodent astrocytes in primary cultures. Pages 397–409.in Gioacobini, E., Vernadakis, A., andShahar, A. (eds.), Tissue culture in Neurobiology, Raven Press, New York.

    Google Scholar 

  11. Booker, J., andSensenbrenner, M. 1981. Growth and cultivation of dissociated neurons and glial cells from embryonic chick, rat and human brain in flask cultures. Neurobiol. 2:97–105.

    Google Scholar 

  12. Hertz, L., Jurrlink, B. H. J., Fosmark, H., andSchousboe, A. 1982. Astrocytes in primary cultures. Pages 175–186,in Pfeiffer, S. E. (eds.), Neuroscience Approached Through Cell Culture, Vol. 1, CRC Press, Boca Raton.

    Google Scholar 

  13. Schousboe, A., Fosmark, H., andHertz, L. 1975. High content of glutamate and of ATP in astrocytes cultured from rat brain hemispheres: Effect of serum withdrawal and of cyclic AMP. J. Neurochem. 25:909–911.

    Google Scholar 

  14. Bullaro, J. C., andBrookman, D. H. 1976. Comaprison of skeletal muscle monolayer cultures initiated with cells dissociated by the vortex and trypsin methods. In Vitro 12:564–570.

    Google Scholar 

  15. Wilkinson, G. N. 1961. Statistical estimations in enzyme kinetics. Biochem. J. 80:324–332.

    Google Scholar 

  16. Bailey-Wood, R., Dodgson, K. S., andRose, F. A. 1970. Purification and properties of two adenosine-5′-phosphate sulfotransferase from rat liver and their possible role in the degradation of 3′-phosphoadenosine-5′-phosphosulphate. Biochim. Biophys. Acta 220:284–299.

    Google Scholar 

  17. Walz, W., Wuttke, W., andHertz, L. 1984. Astrocytes in primary cultures: membrane potential characteristics reveal exclusive potassium conductance and potassium accumulator properties. Brain Res. 292:367–374.

    Google Scholar 

  18. Picker, S., Pieper, C. F., andGoldring, S. 1981. Glial membrane potentials and their relationship to [K+]o in man and guinea pig. J. Neurosurg. 55:347–363.

    Google Scholar 

  19. Yu, P. H., andHertz, L. 1982. Differential expression of type A and type B monoamine oxidase of mouse astrocytes in primary cultures. J. Neurochem. 37:1492–1495.

    Google Scholar 

  20. Yu, P. H., andHertz, L. 1983. Type A and B monoamine oxidase in glial cells in long term culture. Prog. Neuropsychopharmacol. Biol. Psychiat. 7:687–690.

    Google Scholar 

  21. Baranczyk-Kurzma, A., Borchardt, R. T., Schasteen, C. S. andPinnick, C. L. 1981. Phenol sulfotransferase: purification and characterization of the rat brain enzyme. Pages 55–73,in Sandler, M., andUsdin, E. (eds.), Phenolsulfotransferase in Mental Health Research, MacMillian Publishing, London.

    Google Scholar 

  22. Renskers, K. J., Feor, K. D., andRoth, J. A. 1980. Sulfation of dopamine and other biogenic amines by brain phenolsulfotransferase. J. Neurochem. 34:1362–1368.

    Google Scholar 

  23. Sekura, R. D. andJakoby, W. B. 1979. Phenolsulfotransferase. J. Biol. Chem. 254:5658–5663.

    Google Scholar 

  24. Sekura, R. D., andJakoby, W. B. 1981. Aryl sulfotransferase IV from rat liver. Arch Biochem. Biophys. 211:352–359.

    Google Scholar 

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Authors and Affiliations

  1. Psychiatric Research Division, Saskatchewan Health Cancer and Medical Research Building, S7N OWO, Saskatoon, Saskatchewan, Canada

    Peter H. Yu

  2. Department of Pharmacology, University of Saskatchewan, S7N OWO, Saskatoon, Saskatchewan, Canada

    Wolfgang Walz

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  1. Peter H. Yu
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  2. Wolfgang Walz
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Yu, P.H., Walz, W. Occurrence of phenolsulfotransferase in primary glial culture cells of rat. Neurochem Res 10, 983–992 (1985). https://doi.org/10.1007/BF00964634

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