Abstract
This paper investigates the kinetic parameters of Na+−K+-ATPase in glial, neuronal, and synaptosomal enriched fractions isolated from rabbit cerebral cortex. Under normal conditions, kinetic parameters-Vmax and K K+0.5 -of Na+−K+-ATPase are the same in the three fractions, suggesting that this enzyme behaves as the same molecular entity. Following a cryogenic lesion, the alterations of these parameters appear to be different in the different fractions. These data suggest that the same enzyme exhibits various responses when exposed to the same pathological event. The dissimilar lipid composition of the Na+−K+-ATPase environment, and/or different adaptative responses to abnormal ion concentrations in glial, neuronal, and synaptosomal fractions could account for these different responses.
Similar content being viewed by others
References
Bazan, N. G., Politi, E., and Rodriguez de Turco, E. B. 1984. Endogenous pools of arachidonic acid-enriched lipids in cryogenic brain edema. Pages 203–212,in Go, K. G., and Baethmann, A. (eds.), Recent Progress in the Study and Therapy of Brain Edema, Plenum Press, New York.
Bergmeyer, H. U., Bernt, E., and Hess, B. 1963. Lactate dehydrogenase. Page 736,in Bergmeyer, H. U. (ed.), Methods of Enzymatic Analysis, Academic Press, New York.
Booth, R. F., and Clark, J. B. 1978. A rapid method for the preparation of relatively pure metabolically competent synaptosomes from rat brain. Biochem. J. 176:365–370.
Chan, P. H., and Fishman, R. A. 1984. Phospholipid degradation and the early release of polyunsaturated fatty acids in the evolution of brain edema. Pages 193–202,in Go, K. G., and Baethmann, A. (eds.), Recent Progress in the Study and Therapy of Brain Edema, Plenum Press, New York.
Chan, P. H., Quan, S. C., and Fishman, R. A. 1980, Inhibition of rat brain (Na+−K+)-ATPase by polynsaturated fatty acids. Trans. Amer. Soc. Neurochem., 11:120.
Chan, P. H., Yurko, M., and Fishman, R. A. 1982. Phospholipid degradation and cellular edema induced by free radicals in brain cortical slices. J. Neurochem. 38:525–531.
Clasen, R. A., Brown, D. V. L., Leavitt, S., and Hass, G. M. 1953. The production by liquid nitrogen of acute closed cerebral lesions. Surg. Gynecol. Obstet. 96:605–616.
Demopoulos, H. B., Milvy, P., Kakari, S., and Ransohoff, J. 1972. Molecular aspects of membrane structure in cerebral edema. Pages 29–39,in Reulen, H. L., and Schurmann, K. (eds.), Steroids and Brain Edema, Springer Verlag, New York.
Ellman, G. L., Courtney, K. D., Andres, V. Jr. and Featherstone, R. M. 1961. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 7:88–95.
Grisar, T. 1984. Glial and neuronal Na+−K+-pump in epilepsy. Ann. Neurol. 16:S128-S134.
Grisar, T., Franck, G., and Delgado-Escueta, A. V. 1983. Glial contribution to seizures: K+ activation of Na+/K+/ ATPase in bulk isolated glial cells and synaptosomes of epileptogenic cortex. Brain Res. 261:75–84.
Grisar, T., Franck, G., and Delgado-Escueta, A. V. 1983. Na+−K+-ATPase within neurons and glia in the generation of seizures. Pages 199–208,in Delgado-Escueta, A. V., Wasterlain, C. G., Treiman, D. M., and Porter, R. J. (eds.), Advances in Neurology, Vol. 34: Status Epilepticus, Raven Press, New York.
Grisar, T., Frere, J. M., and Franck, G. 1979. Effect of K+ ions on kinetic properties of the (Na+−K+)-ATPase (E.C.3.6.1.3) of bulk isolated glial cells, perikarya and synaptosomes from rabbit brain cortex. Brain Res. 165:87–103.
Hamberger, A., Blomstrand, C., and Lehninger, A. L. 1970. Comparative studies on mitochondria isolated from neuronenriched and glia-enriched fractions of rabbit and beef brain. J. Cell Biol. 45:221–234.
Hamberger, A., and Svennerholm, L. 1971. Composition of gangliosides and phospholipids of neuronal and glial cell enriched fractions. J. Neurochem. 18:1821–1829.
Kimelberg, H. K., Biddlecome, S., Namuri, S., and Bourke, R. S. 1978. ATPase and carbonic anhydrase activities of bulk-isolated neuron, glia and synaptosome fractions from rat brain. Brain Res. 141:305–323.
Kimelberg, H. K., Namuri, S., Biddlecome, S., and Bourke, R. S. 1978. (Na++K+)ATPase,86Rb+ transport and carbonic anhydrase activity in isolated brain cells and cultured astrocytes. Pages 347–357,in Schoffeniels, E., Franck, G., Hertz, L., and Tower, D. B. (eds.), Dynamic Properties of Glia Cells, Pergamon Press, Oxford.
Klatzo, I. 1970. Neuropathological aspects of brain edema. J. Neuropathol. Exp. Neurol. 26:1–13.
Klatzo, I., Piraux, A., and Laskowski, E. J. 1958. The relationship between edema, blood brain barrier and tissue elements in a local brain injury. J. Neuropath. Exp. Neurol. 17:548–564.
Lewin, E. 1972. The production of epileptogenic cortical foci in experimental animals by freezing. Pages 37–49,in Purpura, D. P., Penry, J. K., Towe, O., Woodbury, D. M., and Walter, R. (eds.), Experimental Models of Epilepsy, Raven Press, New York.
Lewin, E., and Bleck, V. 1971. The effect of diphenylhydantoin administration on sodium-potassium-activated ATPase in cortex. Neurology 21:647–651.
Lewin, E., Charles, G., and McCrimmon, A. 1969. Discharging cortical lesions produced by freezing. The effect of anticonvulsants on sodium potassium activated ATPase, sodium and potassium in cortex. Neurology 19:565–569.
Lewin, E., and McCrimmon, A. 1967. ATPase activity in discharging cortical lesions induced by freezing. Arch. Neurol. 16:321–325.
Lewin, E., and McCrimmon, A. 1968. The intraluminar distribution of sodium-potassium activated ATPase activity in discharging cortical lesions induced by freezing. Brain Res. 8:291–297.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193:265–275.
Morrel, F. 1959. Experimental focal epilepsy in animals. Arch. Neurol. 33: vol. 1:141–147.
Morrel, F. 1959/60. Secondary epileptogenic lesions. Epilepsia 1:538–560.
Morrel, F. 1961. Microelectrode studies in chronic epileptic foci. Epilepsia 2:81–88.
Morrel, F., Proctor, F., and Prince, D. A. 1965. Epileptogenic properties of subcortical freezing, Neurology 15:744–751.
Nagata, Y., Mikoshiba, K., and Tsukada, Y. 1974. Neuronal cell body enriched and glial cell enriched fractions from young and adult rat brains: preparation and morphological and biochemical properties. J. Neurochem. 22:493–503.
Prior, W. A., and Stanley, J. P. 1975. A suggested mechanism for the production of malonaldehyde during the autoxidation of polyunsaturated fatty acids: Nonenzymatic production of prostaglandin endoperoxides during autoxidation. J. Org. Chem. 40:3615–3617.
Rapport, R. L., and Ojemann, G. A. 1975. Prophylactically administered phenytoin. Effects on the development of chronic cobalt induced epilepsy in the cat. Arch. Neurol. 32:539–548.
Reulen, H. J. 1981. Pathophysiology of formation and natural resolution of vasogenic brain edema. Pages 31–48,in De Vlieger, M., De Lange, S. A., and Beks, J. W. F. (eds.), Brain Edema. Wiley Medical Publication, John Wiley & Sons, New York, Chichester, Brisbane, Toronto.
Rose, S. P. R. 1967. Preparation of enriched fractions from cerebral cortex containing isolated, metabolically active neuronal and glial cells. Biochem. J. 102:33–43.
Rose, S. P. R., and Sinha, A. K. 1974. Incorporation of amino acids into proteins in neuronal and neuropil fractions of rat cerebral cortex: presence of a rapidly labelling neuronal fraction. J. Neurochem. 23:1065–1076.
Savas, A. H., and Gilbert, J. C. 1984. Possible mechanism of inhibition by lipid peroxidation of ATPase activities of rat cerebral cortex synaptosomes. Arch. Int. Pharmacodyn. 269:4–11.
Sykova, E. 1983. Extracellular K+ accumulation in the central nervous system. Prog. Biophys. Molec. Biol. 42:135–189.
Summer, J. B. 1944. A method for colorimetric determination of phosphorus. Science (NY) 100:413–414.
Sun, A. Y. 1972. The effect of lipoxidation on synaptosomal (Na+−K+)ATPase isolated from the cerebral cortex of squirrel monkey. Biochim. Biophys. Acta 266:350–360.
Walz, W., and Hertz, L. 1983. Functional interactions between neurons and astrocytes. II. Potassium homeostasis at the cellular level. Progress in Neurobiology 20:133–183.
Wheeler, K. P., Walker, J. A., and Barker, D. M. 1975. Lipid requirement of the membrane sodium-plus-potassium ion-dependent adenosine triphosphatase system. Biochem. J. 146:713–722.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Avéret, N., Arrigoni, E., Loiseau, H. et al. Na+−K+-ATPase activity of glial, neuronal, and synaptosomal enriched fractions from normal and freezing-injured rabbit cerebral cortex. Neurochem Res 12, 607–612 (1987). https://doi.org/10.1007/BF00971008
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00971008