Abstract
Qualitative and quantitative changes of mitochondrial membrane proteind during aging were investigated. Free (non-synaptic) mitochondria were purified from rat cerebellum at different ages (4, 8, 12, 16, 20, and 24 months). Mitochondrial outer membrane (OM), inner membrane (IM) and matrix (MX) were separated and the proteins were extracted and analyzed by gel-electrophoresis.
After staining, the gels were scanned densitometrically to quantify the proteins. No significant changes in the quantity of OM or MX protein subunits were observed, while serveral statistically significant quantitative changes in IM proteins with age were found. These age-dependent modifications of inner membrane mitochondrial proteins may play an important role in energy transduction, transport systems and regulatory enzymatic activities in mitochondria.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Miquel, J., Economos, A. C., Fleming, J., and Johnson, J. E. Jr. 1980. Mitochondrial role in cell aging. Exp. Gerontol. 15:575–591.
Fleming, J. E., Miquel, J., Cotrell, S. F., Yenogoyan, I. S., and Economos, A. C. 1982. Is cell aging caused by respiration-dependent injury to the mitochondrial genome? Gerontology 28:44–53.
Vorbeck, M., Martin, A. P., Lang, J. W. J., Smith, J. M., Orr, R., Jr., 1982. Aging dependent modification of lipid composition and lipid structural order parameter of hepatic mitochondria. Arch. Biochem. Biophys. 277:351–361.
Abu-Erreish, C. M., and Sanadi, D. R. 1978. Age-related changes in cytochrome concentration of myocardial mitochondria. Mech. Ageing Devel. 7:425–432.
Starnes, J. W., Beyer, R. W., and Edington, D. W. 1983. Myocardial adaptations to endurance exercise in aged rats. Am. J. Physiol. 245:4560–4566.
Starnes, J. W., Edington, D. W., and Beyer, R. E. 1983. Myocardial protein synthesis during aging and endurance exercise in rats. J. Gerontol. 33:802–810.
Benzi, G., and Giuffrida, A. M. 1987. Changes of synaptosomal energy metabolism induced by hypoxia during aging. Neurochem. Res. 12:149–157.
Benzi, G., Arrigoni, E., Dagani, F., Marzatico, M., Curti, D., Raimondo, S., Dossena, M., Polzatti, M., and Villa, R. F. 1980. Age-dependent modifications of drug interference on the enzymatic activities of the rat brain. Exp. Gerontol. 15:593–603.
Hansford, R. G. 1983. Bioenergetics in aging. Biochim. Biophys. Acta 726:41–80.
Nohl, H., and Kramer, J. 1980. Molecular basis of age-dependent changes in the activity of adenine-nucleotide translocase. Mech. Ageing Devel. 14:137–144.
Hansford, R. G., and Castro, F. 1982. Effect of senescence on Ca2+-ion by heart mitochondria. Mech. Ageing Devel. 19:5–13.
Vitorica, J., Clark, A., Machado, A., and Satrestegui, J. 1985. Impairment of glutamate uptake and absence of alterations in the energy transducing ability of old rat brain mitochondria. Mech. Ageing Devel. 29:255–266.
Miquel, J., and Fleming, J. 1986. Theoretical and experimental support for an “Oxygen radical-Mitochondrial Injury” hypothesis of cell aging, Pages 51–74,in Johnson, J. E., Walford, R., Harman, D., and Miquel, J. (eds.), Free radicals, aging, and degenerative diseases. Vol. 8, Modern Aging Research, Alan Liss, Inc., New York.
De Robertis, E., de Iraldi, A. P., deL. Arnaiz, G. R., and Salganicoff, L. 1962. Cholinergic and non-cholinergic nerve endings in rat brain. 1. Isolation and subcellular distribution of acetylcholine and acetylcholine esterase. J. Neurochem. 9:23–35.
Whittaker, V. P. 1969. The synaptosome,in Lajtha, A. (ed.) Pages 327–364. Handbook of Neurochemistry, Vol. 2, Plenum Press, New York.
Schnaitman, C., and Greenwalt, J. W. 1968. Enzymatic properties of the inner and outer membranes of rat liver mitochondria. J. Cell. Biol. 38:158–175.
Marks, N. 1974. Preparation of brain mitochondrial membranes, Pages 53–77,in Marks, N., and Rodnight, R. (eds.), Methods in Neurochemistry, Vol. 2, Plenum Press, New York.
Laemmli, U. K. 1970. Cleavage of the structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680–685.
Lowry, O. M., Rosebrough, N. J., Farr, A. L., and Randall, R. J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265–275.
De Pierre, J. W., and Ernster, L. 1977. Enzyme topology of intracellular membranes, Pages 207–230,in Snell, E. E., Boyer, P. D., Meister, A., and Richardson, C. C. (eds.), Annual Review of Biochemistry, Vol 46, Annul Reviews Inc., Palo Alto, California.
Dunn, A. J., and Bondy, S. C. 1974. Functional brain chemistry. Page 39, John Wiley & Sons, New York.
Massie, H. R., Baird, M. B., and McMahon, M. M. 1975. Loss of mitochondrial DNA with aging. Gerontology 21:231–237.
Giuffrida Stella, A. M. 1983. Nucleic acids in developing brain, Pages 227–250,in Lajtha, A. (ed.), Handbook of Neurochemistry, Vol 5, Plenum Press, New York.
Bailey, P. J., and Webster, G. C. 1984. Lowered rate of protein synthesis by mitochondria isolated from organisms of increasing age. Mech. Ageing Devel. 24:233–241.
Nohl, H., Breuninger, V., and Hegner, D. 1978. Influence of mitochondrial radical formation on energy-linked respiration. Eur. J. Biochem. 90:385–390.
Marcus, D. L., Ibrahim, N. G., and Fredman, M. I. 1982. Age related decline in the biosynthesis of mitochondrial inner membrane proteins. Exp. Gerontol. 17:333–341.
Fleming, J. E., Melnikoff, P. S., and Bensch, K. G. 1984. Identification of mitochondrial proteins on two dimensional electrophoretic gels of adult Drosophila melanogaster. Biochim. Biophys. Acta 802:340–345.
Fleming, J. E., Melnikoff, P. S., Latter, G. I., Chandia, D., and Bensch, K. G. 1986. Age-dependent changes in the expression of Drosophila mitochondrial proteins. Mech. Ageing Devel. 34:63–72.
Giuffrida Stella, A. M., and Lajtha, A. 1987. Macromolecular turnover in brain during aging. Pages 132–144,in Lechner, H., Agnoli, A., Benzi, G., Tuček, S., Giuffrida Stella, A. M. (eds.), Cerebral Metabolism in Aging and Neurological Disorders, Gerontology, Vol. 33, Karger, S., Basel.
Avola, R., Condorelli, D. F., Ragusa, N., Renis, M., Alberghina, M., and Giuffrida Stella, A. M. 1988. Rate of protein synthesis in various brain regions and subcellular fractions during aging. Neurochem. Res. 13:337–342.
Bradley, M. O., Hayflick, L., and Schumke, R. T. 1976. Protein degradation in human fibroblasts (WI38). Effects of ageing, viral transformation and amino acid analogs. J. Biol. Chem. 251:3521–3529.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Turpeenoja, L., Villa, R.F., Magri, G. et al. Changes of mitochondrial membrane proteins in rat cerebellum during aging. Neurochem Res 13, 859–865 (1988). https://doi.org/10.1007/BF00970754
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00970754