Abstract
A fragment (11–19) of thymosin β4 was found to stimulate phosphodiesterase activity even in the absence of calcium and calmodulin. Half-maximal enzyme activation occurred with 10 nM peptide, and was further increased by phospholipids such as phosphatidylserine. The mechanism of stimulation is an increase in the Vmax of cAMP degradation without a substantial change in the Km for the substrate. In the presence of calcium ions and calmodulin the peptide was also stimulatory.
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Galoyan, A. A., Bobruskin, I. D., Gurvitz, B. Ya., and Abrahamian, G. E. 1989. Calcium-independent activators of cyclic nucleotide phosphodiesterase. Neurokhimiya 8:78–86.
Galoyan, A. A., Bobruskin, I. D., Gurvitz, B. Ya., and Abrahamian, G. E. 1990. Calcium-independent allosteric modulation of cyclic nucleotide phosphodiesterase activity. Neurokhimiya 9:110–118.
Abrahamian, G. E., Isadjanyan, M. A., Chailyan, S. G., Kirakosova, A. S., and Galoyan, A. A. 1992. Interaction of (11–19) synthetic fragment of C-modulin 3 with calmodulin-sensitive enzyme. Evidence for calcium-independent stimulation. Neurokhimiya 11:10–20.
Galoyan, A. A., Gurvitz, B. Ya., Shuvalov, L. A., Davis, M. T., Shively, J. E., and Lee, T. D. 1992. A hypothalamic activator of calmodulin-dependent enzymes is thymosin β4 (1–39). Neurochem. Res. 17:773–777.
Bobruskin, I. D., Saichin, S. M., Muratova, M. V., Baranova, L. A., and Severin, E. S. 1987. Regulation of cyclic nucleotide phosphodiesterase activity by BSA. Biochemistry 52:1344–1352.
Abrahamian, G. E., Chailyan, S. G., Kirakosova, A. S., Isdjanyan, M. A., and Galoyan, A. A. 1992. Purification of calmodulin-stimulated phosphodiesterase from hypothalamus by affinity HPLC. Neurokhimiya 11:21–28.
Gopalacrishna, R., and Anderson, W. B. 1982. Ca2+ induced hydrophobic site on calmodulin: application for purification of calmodulin by phenylsepharose affinity chromatography. Biochem. Biophys. Res. Commun. 104:830–836.
Thompson, W. J., and Appleman, M. M. 1971. Preparation and characterization of rat brain 3′–5′cyclic nucleotide phosphodiesterase. Biochemistry 10:311–316.
Abrahamian, G. E., Chailyan, S. G., and Galoyan, A. A. 1992. An affinity high performance liquid chromatography as method for study of interaction of calmodulin-stimulated enzymes with bioregulators and drugs. Neurokhimiya 11:72–91.
Cheung, W. Y. 1980. Calmodulin plays a pivotal role in cellular regulation. Science 207:19–27.
Novel Calcium Binding Proteins, Fundamentals and Clinical Implications, in Heizman, C. W. (ed.), Springer-Verlag, Berlin, New York, Paris.
Horecker, B. L., and Morgan, J. 1984. Ubiquitous distribution of thymosin β4 and related peptides in verbebrate cell tissues. Pages 15–35, in Pick, E. and Landy, M. (eds.), Lymphokinenes,
Low, T. L. K. and Goldstein, A. L. 1979. The chemistry and biology of thymosin II amino acid sequence analysis of thymosin α1 and polypeptide β1. J. Biol. Chem. 254:987–995.
Hannappel, E. 1986. One-step procedure for the determination of thymosin β4 in small tissue samples and its separation from other thymosin β4-like peptides by HPLC. Anal. Biochem. 156:390–396.
Xu, G.-J., Hannappel, E., Morgan, J., Hempstead, J., and Horecker, B. L. 1982. Synthesis of thymosin β4 by peritoneal macrophages and adherent spleen cells. Proc. Natl. Acad. Sci. U.S.A. 79:4006–4009.
Goodall, G. J., Hempstead, J. L., and Morgan, J. I. 1983. Production and characterization of antibodies to thymosin β4. J. Immunol. 131:821–825.
Hannappel, E., Xu, G.-J., Morgan, J. I., Hempstead, J. L., and Horecker, B. L. 1982. Thymosin β4: a ubiquitous peptide in rat and mouse tissues. Proc. Natl. Acad. Sci. U.S.A. 79:2172–2175.
Low, T. L. K., Hu, S.-K., and Goldstein, A. L. 1981. Complete amino acid sequence of bovine thymosin β4: a thymic hormone that induces terminal deoxynucleotidyl transferase activity in thymocyte populations. Proc. Natl. Acad. Sci. U.S.A. 78:1162–1166.
Rebar, R. W., Miyace, A., Low, T. L. K., and Goldstein, A. L. 1981. Thymosin stimulates secretion of luteinizing hormone-releasing factor. Science 214:669–671.
Lugo, D. I., Chen, Sh.-Ch., Hall, A. K., Reza, Z., Hempstead, J. L., and Morgan, J. I. 1991. Developmental regulation of β-thymosins in the rat central nervous system. J. Neurochem. 56:457–461.
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Galoyan, A.A., Abrahamian, G.E., Chailyan, S.G. et al. Calcium- and calmodulin-independent modulation of calmodulin-sensitive hypothalamic cyclic nucleotide phosphodiesterase activity by the (11–19) fragment of thymosin β4. Neurochem Res 19, 451–456 (1994). https://doi.org/10.1007/BF00967323
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DOI: https://doi.org/10.1007/BF00967323