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Effect of Osmolality and myo-Inositol Deprivation on the Transport Properties of myo-Inositol in Primary Astrocyte Cultures

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Abstract

myo-Inositol uptake measured in primary astrocyte cultures was saturable in the presence of Na+ with a Km of 13–18 μM and a Vmax of 9.4 nmoles/mg protein/hour in myo-inositol-fed cells, indicating a high affinity transport system. In myo-inositol-deprived cells, Km was about 53 μM with a Vmax of 13.2 nmoles/mg protein/hour. Decreasing osmolality decreased the Vmax to about 1.9 nmoles/mg protein/hour whereas increasing osmolality increased Vmax about 5-fold, while Kms were essentially unchanged in myo-inositol fed cells. In cells deprived of myo-inositol, Vmax decreased in hypotonic medium and increased in hypertonic medium almost 10-fold, but with more than a doubling of the Km regardless of the osmolality. Glucose (25 mM) inhibited myo-inositol uptake 51% whereas the other hexoses used inhibited uptake much less. Our findings indicate that myo-inositol uptake in astrocytes occurs through an efficient carrier-mediated Na+-dependent co-transport system that is different from that of glucose and its kinetic properties are affected by myo-inositol availability and osmotic stress.

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Authors and Affiliations

  1. Research Laboratories, Veterans Affairs Medical Center, Miami, Florida

    R. E. Isaacks, C. Y. Kim & M. D. Norenberg

  2. Department of Medicine, University of Miami School of Medicine, Miami, Florida, 33125

    R. E. Isaacks

  3. Department of Pathology, University of Miami School of Medicine, Miami, Florida, 33125

    A. S. Bender & M. D. Norenberg

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Isaacks, R.E., Bender, A.S., Kim, C.Y. et al. Effect of Osmolality and myo-Inositol Deprivation on the Transport Properties of myo-Inositol in Primary Astrocyte Cultures. Neurochem Res 22, 1461–1469 (1997). https://doi.org/10.1023/A:1021950311308

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