Abstract
Five hybrids reactive with monoclonal antibodies against human leukocyte common antigen (T-200) and/or lymphocyte function-associated antigen 1 (LFA-1) β subunit were obtained from the fusion of human blood lymphocytes or T-cell chronic lymphocytic leukemia cells with BW5147 mouse T-cell leukemia cells. Chromosome analyses of 20 clones showed concordance between the presence of human chromosomes 1 and 21 and the expression of T-200 and LFA-1 β subunit, respectively. Confirmation of human chromosomes in the hybrids was made by the electrophoretic analyses of phosphoglucomutase 1 for chromosome1and Superoxide dismutase A for chromosome 21. The results suggested that the presence of human chromosomes 1 and 21 was essential for the expression of T-200 and LFA-1 β subunit, respectively.
Literature cited
Tsuge, I., Utsumi, K.R., Ueda, R., Takamoto, S., and Takahashi, T. (1985).Somat. Cell Mol. Genet. 11:217–222.
Human Gene Mapping 8. (1985).Cytogenet. Cell Genet. 40:64.
Morishima, Y., Ogata, S., Collins, N.H., Dupon, B., and Lloyd, K.O. (1982).Immunogenetics 15:529–535.
Omary, M.B., Trowbridge, I.S., and Battifora, H.A. (1980).J. Exp. Med. 152:842–852.
Dalchau, R., Kirkley, J., and Fabre, J.W. (1980).Eur. J. Immunol. 10:737–744.
Sparrow, R.L., and Mckenzie, I.F.C. (1983).Transplantation 36:166–171.
Sanchez-Madrid, F., Nagy, J.A., Robbins, E., Simon, P., and Springer, T.A. (1983).J. Exp. Med. 158:1785–1803.
Hildreth, J.E.K., Gotch, F.M., Hildreth, P.D.K., and McMichael, A.J. (1983).Eur. J. Immunol. 13:202–208.
Springer, T.A., and Anderson, D.C. (1986). InLeukocyte Typing II, Vol. 3, (ed.) Reinharz, E.L., Haynes, B.F., Nadler, L.M., Bernstein, I.D. (Springer-Verlag, Berlin), pp. 55–68.
Retting, W.J., Dracopoli, N.C., Goetzger, T.A., Spengler, B.A., Biedler, J.L., Oettgen, H.F., and Old, L.J. (1984).Somat. Cell Mol. Genet. 10:297–305.
Carey, T.E., Takahashi, T., Resnick, L.A., Oettgen, H.F., and Old, L.J. (1976).Proc. Natl. Acad. Sci. U.S.A. 73:3278–3282.
Ueda, R., Tanimoto, M., Takahashi, T., Ogata, S., Nishida, K., Namikawa, R., Nishizuka, Y., and Ota, K. (1982).Proc. Natl. Acad. Sci. U.S.A. 79:4386–4390.
Seabright, M. (1971).Lancet 2:971–972.
Harris, H., and Hopkinson, D.A. (1976).Handbook of Enzyme Electrophoresis in Human Genetics, (Elsevier/North-Holland, Amsterdam).
Barbosa, J.A., Mentzer, S.J., Kamarck, M.E., Hart, J., Biro, P.A., Strominger, J.L., and Burakoff, S.J. (1986).J. Immunol. 136:3085–3091.
Shen, F.-W., Saga, Y., Litman, G., Freeman, G., Tung, J.-S., Cantor, H., and Boyse, E.A. (1985).Proc. Natl. Acad. Sci. U.S.A. 82:7360–7367.
Marlin, S.D., Morton, C.C., Anderson, D.C., and Springer, T.A. (1986).J. Exp. Med. 164:855–867.
Arnaout, M.A., Dana, N., Pitt, J., and Todd, R.F., III. (1985).Fed. Proc. 44:2664–2670.
Suomalainen, H.A., Gahmberg, C.G., Patarroyo, M., Beatty, P.G., and Schroder, J. (1986).Somat. Cell Mol. Genet. 12:297–302.
Raziuddin, A., Sarkar, F.H., Dutkowski, R., Shulman, L., Ruddle, F.H., and Gupta, S.L. (1984).Proc. Natl. Acad. Sci. U.S.A. 81:5504–5508.
Honey, N.K., Sakaguchi, A.Y., Lalley, P.A., Quinto, C., MacDonald, R.J., Craik, C., Bell, G.I., Rutter, W.J., and Naylor, S.L. (1984).Somat. Cell Mol. Genet. 10:377–383.
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Akao, Y., Utsumi, K.R., Naito, K. et al. Chromosomal assignments of genes coding for human leukocyte common antigen, T-200, and lymphocyte function-associated antigen 1, LFA-1β subunit. Somat Cell Mol Genet 13, 273–278 (1987). https://doi.org/10.1007/BF01535209
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DOI: https://doi.org/10.1007/BF01535209