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The use of irradiation and fusion gene transfer (IFGT) hybrids to isolate DNA clones from human chromosome region 9q33-q34

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Somatic Cell and Molecular Genetics

Abstract

We have generated somatic cell hybrids containing fragments of human chromosome arm 9q by an irradiation and fusion technique. No selection for human material was imposed, but of 23 clones analyzed most contained human DNA sequences and many contained multiple fragments of the human chromosome arm. A hybrid that appears to contain only two small fragments of human DNA from the regions q33 and q34 has been used as a source from which to clone probes specific to those areas of the chromosome.

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Literature cited

  1. Porteus, D. (1987).Trends Genet. 3177–182.

    Google Scholar 

  2. Estiville, X., Farrall, M., Scambler, P.J., Bell, G.M., Hawley, K.M.F., Lench, N.J., Bates, G.P., Kruyer, H.C., Frederick, P.A., Stanier, P., Watson, E.K., Williamson, R. and Wainwright, B.J. (1987).Nature 326840–845.

    Google Scholar 

  3. Pritchard, C.A., and Goodfellow, P.N. (1986).EMBO J. 5979–985.

    Google Scholar 

  4. Goss, S.J., and Harris, H. (1975).Nature 255680–684.

    Google Scholar 

  5. Benham, F., Hart, K., Crolla, J., Bobrow, M., Francavilla, M. and Goodfellow, P.N. (1989).Genomics 4509–517.

    Google Scholar 

  6. Pritchard, C.A., Casher, D., Uglum, E., Cox, D.R., and Myers, R.M. (1989).Genomics 4408–418.

    Google Scholar 

  7. Rose, E.A., Glaser, T., Jones, C., Smith, C.L., Lewis, W.H., Call, K.M., Minden, M., Champagne, E., Bonetta, L., Yeger, H., and Houseman, D. (1990).Cell 60495–508.

    Google Scholar 

  8. Fryer, A.E., Chalmers, A., Connor, J.M., Fraser, I., Povey, S., Yates, A.D., Yates, J.R.W., and Osborne, J.P. (1987).Lancet 1659–660.

    Google Scholar 

  9. Jones, C., and Kao, F.T. (1984).Cytogenet. Cell Genet. 37499–500.

    Google Scholar 

  10. Bishop, C.E., Guellen, G., Geldworth, D., Voss, R., Fellous, M., and Weissenbach, J. (1982).Nature 303831–832.

    Google Scholar 

  11. Goodfellow, P.N., Pritchard, C.A., and Banting, G.S. (1988). InGenome Analysis: A Practical Approach (ed.) Davies, K.E. (IRL Press, Oxford), pp. 1–17.

    Google Scholar 

  12. Harris, H., and Hopkinson, D.A. (1979).Handbook of Enzyme Electrophoresis in Human Genetics (North Holland Press, Amsterdam).

    Google Scholar 

  13. Church, G.M., and Gilbert, W. (1984).Proc. Natl. Acad. Sci. U.S.A. 811991–1995.

    Google Scholar 

  14. Abbott, C., and Povey, S. (1991).Genomics 973–77.

    Google Scholar 

  15. Wallis, J., Williamson, R., and Chamberlain, S. (1990).Hum. Genet. 8598–100.

    Google Scholar 

  16. Little, P.F.R. (1987). InDNA Cloning, Vol. III, (ed.) Glover, D.M. (TRL Press, Oxford), pp. 19–42.

    Google Scholar 

  17. Maniatis, T., Fritsch, E.F., and Sambrook, J. (1982).Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory, Cold Spring Harbor, New York).

    Google Scholar 

  18. Griffin, D.K., Leigh, S.E.A., and Delhanty, J.D.A., (1990).Genes Chromosomes Cancer 1281–283.

    Google Scholar 

  19. Wheater, R.F., and Roberts, S.H.J. Med. Genet. (1987).24113–115.

    Google Scholar 

  20. Smith, M., and Simpson, N.E. (1989).Cytogenet. Cell Genet. 51202–225.

    Google Scholar 

  21. Povey, S., Attwood, J., Janssen, L.A.J., Burley, M., Smith, M., Flodman, P., Morton, N.E., Edwards, J.H., Sampson, J.R., Yates, J.R.W., Haines, J.L., Amos, J., Short, M.P., Sandkuyl, L.A., Halley, D.J.J., Fryer, A.E., Bech-Hansen, T., Mueller, R., Al-Ghazali, L., Super, M., and Osbourne, J. (1991).Ann. N.Y. Acad. Sci. 615298–305.

    Google Scholar 

  22. Smith, M., Smalley, S., Cantor, R., Pandolfo, M., Gomez, M.I., Baumann, R., Flodman, P., Yoshiyama, K., Nakamura, Y., Julier, C., Dumars, K., Haines, J., Trofatter, J., Spence, M.A., Weeks, D., and Conneally, M. (1990).Genomics 6105–114.

    Google Scholar 

  23. Cox, D.R., Burmeister, M., Price, E.R., Kim, S., and Myers, R.M. (1990).Science 250245–250.

    Google Scholar 

  24. Falk, C.T. (1991).Genomics 9120–123.

    Google Scholar 

  25. Schleutermann, D.A., Bias, W.B., Murdoch, J.L., and McKusick, V.A. (1969).Am. J. Hum. Genet. 21606–630.

    Google Scholar 

  26. Kramer, P.L., de Leon, D., Ozelius, L., Risch, N., Bressman, S.B., Brin, M.F., Schuback, D.E., Burke, R.E., Kwiatkowski, D.J., Shale, H., Gusella, J.F., Breakefield, X.O., and Fahn, S. (1990).Ann. Neurol. 27114–120.

    Google Scholar 

  27. Lathrop, G.M., Nakamura, Y., O'Connell, P., Leppert, M., Woodward, S., Lalouel, J.-M., and White, R. (1988).Genomics 3361–366.

    Google Scholar 

  28. Kumlin-Wolff, E., Nakamura, Y., Myers, R., Eldridge, J.R., O'Connell, P., Leppert, M., Lathrop, G.M., Lalouel, J-M., and White, R. (1987).Nucleic Acids Res. 1510611.

    Google Scholar 

  29. Daiger, S.P., Hoffman, N.S., Wildin, R.S., and Su, T.-S. (1984).Am. J. Hum. Genet. 36736–749.

    Google Scholar 

  30. Hsu, L.C., Tani, K., Fujiyoshi, T., Kurachi, K., and Yoshida, A. (1985).Proc. Natl. Acad. Sci. U.S.A. 823771–3775.

    Google Scholar 

  31. Wetmur, J.G., Bishop, D.F., Cantelmo, C., and Desnick, R.J. (1986).Proc. Natl. Acad. Sci. U.S.A. 837703–7707.

    Google Scholar 

  32. McMahon, A.P., Giebelhaus, D.H., Champion, J.E., Bailes, J.A., Lacey, S., Carritt, B., Henchman, S.K., and Moon, R.T. (1987).Differentiation 3468–78.

    Google Scholar 

  33. Wang, J.Y.T., and Baltimore, D. (1983).Mol. Cell. Biol. 3773–779.

    Google Scholar 

  34. Lamouroux, A., Vigny, A., Faucon Biguet, N., Darmon, M.C., Franck, R., Henry, J.-P., and Mallet, J. (1987).EMBO J. 63931–3937.

    Google Scholar 

  35. Shafit-Zagardo, B., Maio, J.J., and Brown, F.L. (1982).Nucleic Acids Res. 103175–3193.

    Google Scholar 

  36. Lalley, P.A., Davisson, M.T., Graves, J.A.M., O'Brien, S.J., Womack, J.E., Roderick, T.H., Creau-Goldberg, N., Hillyard, A.L., Doolittle, D.P., and Rogers, J.A. (1989).Cytogenet. Cell Genet. 51503–532.

    Google Scholar 

  37. Wilkes, D., Shaw, J., Anand, R., Riley, J., Winter, P., Wallis, J., Driesel, A.G., Williamson, R., and Chamberlain, S. (1991).Genomics 990–95.

    Google Scholar 

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Florian, F., Hornigold, N., Griffin, D.K. et al. The use of irradiation and fusion gene transfer (IFGT) hybrids to isolate DNA clones from human chromosome region 9q33-q34. Somat Cell Mol Genet 17, 445–453 (1991). https://doi.org/10.1007/BF01233168

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  • DOI: https://doi.org/10.1007/BF01233168

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