Abstract
Grafts of various types of cells have been performed using bioresorbable polymer matrices. These synthetic fibers are degraded by hydrolysis into normal metabolic intermediates and induce a number of events that are conducive to healing and/or repair, the most important of which may be angiogenesis. The use of biodegradable meshes to deliver genetically altered cells was studied. A β-galactosidase gene was inserted into Long-Evans rat bone marrow stromal (BMS) cells or fibroblasts derived from C57BL/6J mouse embryos using the retroviral vector LNL-SLXβgal. Expression was monitored using X-gal staining. X-gal+ cells from monolayer cultures were seeded onto either polyglycolic acid (PGA) or polyglactin (PGL) biodegradable meshes and grown to confluence. Two types of grafts were performed: (1) embryonic C57BL/6J mouse fibroblasts (EMF) into either nude mice or adult C57BL/6J mice, and (2) Long-Evans rat BMS into Long-Evans rats. β-Galactosidase activity was found for up to 152 days for EMF in nude mice, 123 days for EMF in adult C57BL/6J mice, and 90 days for grafts of syngeneic BMS cells into Long-Evans rats. Noninfected cells grafted using the same methods did not stain with X-gal.
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Literature cited
Dick, J.E., Magli, M.C., Huszar, D., Phillips, R.A., and Bernstein, A. (1985).Cell 4271–79.
Keller, G., Paige, C., Gilboa, E., and Wagner, E.F. (1985).Nature 318149–154.
St. Louis, D., and Verma, I.M. (1988).Proc. Natl. Acad. Sci. U.S.A. 853150–3154.
Selden, R.F., Skoskiewicz, M.J., Burke Howie, K., Russell, P.S., and Goodman, H.M. (1987).Science 236714–718.
Scharfmann, R., Axelrod, J.H., and Verma, I.M. (1991).Proc. Natl. Acad. Sci. U.S.A. 884626–4630.
Wilson, J.M., Birinyi, L.K., Salomon, R.N., Libby, P., Callow, A.D., and Mulligan, R.C. (1989).Science 2441344–1346.
Short, M.P., Choi, B.C., Lee, J.K., Malick, A., Breakefield, X.O., and Martuza, R.L. (1990).J. Neurosci. Res. 27427–433.
Wolff, J.A., Malone, R.W., Williams, P., Chong, W., Acsadi, G., Jani, A., and Felgner, P.L. (1990).Science 2471465–1468.
Nabel, E.G., Plautz, G., Boyce, F.M., Stanley, J.C., and Nabel, G.J. (1989).Science 2441342–1344.
Anderson, K.D., Thompson, J.A., DiPietro, J.M., Montgomery, K.T., Reid, L.M., and Anderson, W.F. (1989).Somat. Cell Mol. Genet. 15215–227.
Gilbert, J. C., Takada, T., Stein, J.E., and Vacanti, J.P. (1992).Transplantation (in press).
Nabel, E.G., Plautz, G., and Nabel, G.J. (1990).Science 2491285–1287.
Jaenisch, R. (1980).Cell 19181–188.
Kaneda, Y., Iwai, K., and Uchida, T. (1989).J. Biol. Chem. 26412,126–12,129.
Chang, P.L., Capone, J.P., and Brown, G.M. (1990).Mol. Biol. Med. 7461–470.
Vacanti, J.P., Morse, M.A., Saltzman, W.M., Domb, A.J., Perez-Atayde, A., and Langer, R. (1988).J. Pediatr. Surg. 233–9.
Cima, L.G., Vacanti, J.P., Vacanti, C., Ingber, D., Mooney, D., and Langer, R. (1991).J. Biomech. Eng. 113143–151.
Cooper, M.L., Hansbrough, J.F., Spielvogel, R.L., Cohen, R., Bartel, R., and Naughton, G. (1991).Biomaterials 12243–248.
Cooper, M.L., Hansbrough, J.F., Spielvogel, R., Cohen, R., Bartel, R.L., and Naughton, G. (1992).Surgery 111438–446.
Craig, P.H., Williams, J.A., Davis, K.W., Magoun, A.D., Levy, A.J., Bogdansky, S., and Jones, J.P., Jr. (1975).Surg. Gynecol. Obstet. 1411–10.
Halberstadt, C., Bartel, R.L., Slivka, S.R., Underwood, J., Zeigler, F., and Naughton, G.K. (1992).J. Invest. Dermatol. 98601.
Bender, M.A., Palmer, T.D., Gelinas, T.E., and Miller, A.D. (1987).J. Virol. 611639–1646.
Weiss, L. (1974).Anat. Rec. 186161–184.
Horwitz, J.P., Chua, J., Curby, R.J., Tomson, A.J., DaRooge, M.A., Fisher, B.E., Mauricio, J., and Klundt, I. (1964).J. Med. Chem. 7574–575.
Naughton, B.A., Liu, P., Kolks, G.A., Arce, J.M., Piliero, S.J., and Gordon, A.S. (1980).Am. J. Physiol. 238E245-E252.
Naughton, B.A., Dornfest, B.S., Bush, M.E., Carlson, C.A., and Lapin, D.M. (1990).J. Lab. Clin. Med. 116498–507.
Williams, D.A., Lemischka, I.R., Nathan, D.G., and Mulligan, R.C. (1984).Nature 310476–480.
McIvor, R.S., Johnson, M.J., Miller, A.D., Pitts, S., Williams, S.R., Valerio, D., Martin, D.W., Jr., and Verma, I.M. (1987).Mol. Cell. Biol. 7838.
Peng, H., Armentano, D., MacKenzie-Graham, L., Shen, R-F, Darlington, G., Ledley, F., and Woo, S.L.C. (1988).Proc. Natl. Acad. Sci. U.S.A. 858146–8150.
Wilson, J.M., Jefferson, D.M., Chowdhury, J.R., Novikoff, P.M., Johnston, D.E., and Mulligan, R.C. (1988).Proc. Natl. Acad. Sci. U.S.A. 853014–3018.
Plautz, G., Nabel, E.G., and Nabel, G.J. (1991).New Biol. 3709–715.
Miller, A.D., Ong, E.S., Rosenfeld, M.G., Verma, I.M., and Evans, R.M. (1984).Science 225993–998.
Axelrod, J.H., Read, M.S., Brinkhous, K.M., and Verma, I.M. (1990).Proc. Natl. Acad. Sci. U.S.A. 875173–5177.
Palmer, T.D., Thompson, A.R., and Miller, A.D. (1989).Blood 73438–445.
Tani, K., Ozawa, K., Ogura, H., Takahashi, T., Okano, A., Watari, K., Matsudaira, T., Tajika, K., Asono, S., and Takaku, F. (1989).Blood 741274–1280.
Garver, R.I., Jr., Chytil, A., Karlsson, S., Fells, G.A., Brantly, M.L., Courtney, M., Kantoff, P.W., Nienhuis, A.W., Anderson, W.F., and Crystal, R.G. (1987).Proc. Natl. Acad. Sci. U.S.A. 841050–1054.
Hayflick, L. (1965).Exp. Cell Res. 37614–636.
Sudhakaran, P.R., Stamatoglou, S.C., and Hughes, R.C. (1986).Exp. Cell Res. 167505–516.
Schmidhauser, C., Bissell, M.J., Myers, C.A., and Casperson, G.F. (1990).Proc. Natl. Acad. Sci. U.S.A. 879118–9122.
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Naughton, B.A., Dai, Y., Sibanda, B. et al. Long-term expression of a retrovirally introduced β-galactosidase gene in rodent cells implanted in vivo using biodegradable polymer meshes. Somat Cell Mol Genet 18, 451–462 (1992). https://doi.org/10.1007/BF01233085
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DOI: https://doi.org/10.1007/BF01233085