Abstract
In this study we investigated the expression of primate galactokinase in somatic cell hybrids between a thymidine kinase-deficient mouse cell line and two different primate cell lines, one of which was derived from African green monkey kidney cells and the other from chimpanzee fibroblasts. All the African green monkey-mouse hybrid clones, selected in HAT medium, expressed monkey galactokinase activity and contained a monkey chromosome similar to a human E-group chromosome. When these clones were backselected in medium containing 5-bromodeoxyuridine, both this chromosome and the monkey galactokinase activity were lost. All the hybrid clones between mouse and chimpanzee cells, which were selected in HAT medium, contained the chimpanzee chromosome 17 and expressed chimpanzee galactokinase activity. These results indicate that the linkage relationship between galactokinase and thymidine kinase has been maintained in 3 divergent primate species—man, chimpanzee, and Old World monkey.
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Literature cited
Turleau, C., and deGrouchy, J. (1973).Humangenetik 20:151–157.
Bobrow, M., and Madan, K. (1973).Cytogenet. Cell Genet. 12:107–116.
Hamerton, J.L., Klinger, H.P., Mutton, D.E., and Lang, E.M. (1963).Cytogenetics 2:240–263.
Finaz, C., Turleau, C., deGrouchy, J., VanCong, N., Rebourcet, R., and Frezal, J. (1973).Biomedicine 19:526–531.
Williams, C.A., Jr. (1964). InEvolutionary and Biology of Primates, Buettner, J. (ed.), Vol. 2, Academic Press, New York, pp. 25–74.
Turleau, C., deGrouchy, J., and Klein, M. (1972).Ann. Genet. 15:225–240.
Miller, O.J., Allderdice, P.W., Miller, D.A., Breg, W.R., and Migeon, B.R. (1971).Science 173:244–245.
Boone, C., Chen, T., and Ruddle, F.H. (1972).Proc. Natl. Acad. Sci. U.S.A. 69:510–514.
Orkwiszewski, K.G., Tedesco, T.A., and Croce, C.M. (1974).Nature 252:60–62.
Elsevier, S.M., Kucherlapati, R.S., Nichols, E.A., Creagan, R.P., Giles, R.E., Ruddle, F.H., Willecke, K., and McDougall, J.K. (1974).Nature 251:633–636.
Littlefield, J.W. (1966).Exp. Cell Res. 41:190–196.
Croce, C.M., Huebner, K., and Koprowski, H. (1974),Proc. Natl. Acad. Sci. U.S.A. 71:4116–4119.
Croce, C.M., Koprowski, H., and Eagle, H. (1972).Proc. Natl. Acad. Sci. U.S.A. 69:1953–1956.
Seabright, M. (1971).Lancet 2:971–972.
Croce, C.M., Knowles, B.B., and Koprowski, H. (1973).Exp. Cell Res. 82:457–461.
Bobrow, M., Madan, K., and Pearson, P.L. (1972).Nature (London) New Biol. 238:122–124.
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Orkwiszewski, K.G., Tedesco, T.A., Mellman, W.J. et al. Linkage relationship between the genes for thymidine kinase and galactokinase in different primates. Somat Cell Mol Genet 2, 21–26 (1976). https://doi.org/10.1007/BF01539239
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DOI: https://doi.org/10.1007/BF01539239