Skip to main content
SpringerLink
Log in

Thermodynamic analysis of the interaction of proflavin with the deoxytetraribonucleotide 5′-d(GpCpGpC) according to proton magnetic resonance data

  • Published:
Theoretical and Experimental Chemistry Aims and scope

Abstract

The temperature dependences of the chemical shifts of the proflavin protons in a mixture with the deoxytetraribonucleoside diphosphate 5′-d(GpCpGpC) in aqueous solution were investigated on a pulse NMR spectrometer (500 MHz). A procedure is proposed for calculating the mole fractions of various associates in solution as a function of the temperature. The Gibbs free energies, enthalpies, and entropies of the reactions forming the 1∶1, 1∶2, 2∶1, and 2∶2 complexes of proflavin with the tetranucleotide were determined. The results are evidence of a vital role of hydrophobic interactions in the formation of complexes of the dye with the duplex of the tetramer.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature cited

  1. A. N. Veselkov and L. N. Dymant, Teor. Éksp. Khim., 28, No. 5/6, 424–429 (1992).

    Google Scholar 

  2. A. N. Veselkov, D. Dévis, and L. N. Dymant, Biopolim. Kletka, 8, No. 1, 23–35 (1992).

    Google Scholar 

  3. A. N. Veselkov, L. N. Dymant, and S. F. Baranovskii, Mol. Biol., 21, No. 4, 1110–1116 (1987).

    Google Scholar 

  4. A. N. Veselkov, L. N. Dymant, and S. S. Dreval'; Teor. Éksp. Khim., 23, No. 3, 373–376 (1987).

    Google Scholar 

  5. P. D. Ross and S. Subramanian, Biochemistry, 20, 3096–3102 (1981).

    Google Scholar 

  6. J. B. Caires, Biopolymers, 24, 403–419 (1985).

    Google Scholar 

  7. L. A. Marky, K. S. Blumenfeld, and K. J. Breslauer, Nucl. Acids Res., 11, 2857–2870 (1983).

    Google Scholar 

  8. L. A. Marky and K. J. Breslauer, Biopolymers, 21, 2185–2194 (1982).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Sevastopol' Instrument-Making Institute, USSR

    L. N. Dymant & A. N. Veselkov

Authors
  1. L. N. Dymant
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. N. Veselkov
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Translated from Teoreticheskaya i Éksperimental' naya Khimiya, Vol. 28, Nos. 5–6, pp. 430–435, September–December, 1992.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dymant, L.N., Veselkov, A.N. Thermodynamic analysis of the interaction of proflavin with the deoxytetraribonucleotide 5′-d(GpCpGpC) according to proton magnetic resonance data. Theor Exp Chem 28, 329–332 (1993). https://doi.org/10.1007/BF00532105

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00532105

Keywords

Search

Navigation