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Über Veränderungen der Feinstrukturen von Bakterien unter der Einwirkung von Chloramphenicol

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Zusammenfassung

Durch Einwirkung von Chloramphenicol (Paraxin) auf die grampositiven Staphylokokken lassen sich starke Zellwandverdickungen induzieren, die auf eine Hemmung des Wachstums der Bakterienzelle bei gleichzeitig fast ungehemmt weiterlaufender Synthese von Zellwandmaterial zurückzuführen sind.Die Masse der Wandsubstanz kann daher als Maß für den Grad der induzierten Wachstumshemmung herangezogen werden.

Durch Chloramphenicol können bei Staphylokokken auch die Membrankörper und die Chromosomenareale verändert und die Ausbildung myelinartiger Strukturen induziert werden. Eine aktive Beteiligung der Membrankörper an der Zellwandsynthese wurde wahrscheinlich gemacht.

Im Gegensatz zu den grampositiven Staphylokokken reagierten gramnegative Bakterien wie E. coli auf Chloramphenicol mit andersartigen Zellveränderungen.

Summary

By the influence of Chloramphenicol (Paraxin) on grampositive staphylococci, large cellwall thickenings can be induced, that are to be referred to a growth-restriction of the germ cell cytoplasme with an at the same time almost unrestrictedly continuing synthesis of cell wall material.The mass of the cell wall substance can therefore be referred to as a measure for the degree of induced growth restriction.

Plasmalemmasomes and chromosom areas of staphylococci may become altered by Chloramphenicol, and the formation of myelin figures may be induced. An active participation of the plasmalemmasomes on the synthesis of the cell wall substance has been made likely.

In contrast to the grampositive staphylococci gramnegative germs like E. coli react on Chloramphenicol with other cell alterations.

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Authors and Affiliations

  1. Robert Koch-Institut des Bundesgesundheitsamtes Berlin, Germany

    P. Giesbrecht & H. Ruska

  2. Institut für Biophysik und Elektronenmikroskopie der Universität Düsseldorf, Germany

    P. Giesbrecht & H. Ruska

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  1. P. Giesbrecht
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  2. H. Ruska
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Durchgeführt mit Unterstützung der C. F. Boehringer & Söhne GmbH Mannheim-Waldhof.

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Giesbrecht, P., Ruska, H. Über Veränderungen der Feinstrukturen von Bakterien unter der Einwirkung von Chloramphenicol. Klin Wochenschr 46, 575–582 (1968). https://doi.org/10.1007/BF01747836

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