Summary
A method is described which allows the identification of proliferating monocytic precursors in smears of aspirated bone marrow. The method is based on the combination on the same slide of specific enzyme-cytochemical techniques for the demonstration of human monocytes and monocytic precursors with microautoradiography.
Zusammenfassung
Es wird ein Verfahren angegeben, das die Darstellung proliferierender monocytärer Vorstufen in Knochenmarksausstrichen des Menschen gestattet. Diese Methode beruht auf der Kombination der spezifischen enzymcytochemischen Darstellung von Monocyten und Monocytenvorstufen mit mikroautoradiographischen Verfahren am selben Präparat.
References
Braunsteiner, H., u.F. Schmalzl: Das Problem der Monozyten. Dtsch. med. Wschr.93, 456 (1968).
Everett, N. B., andR. W. Tyler: Studies of lymphocytes: relationship to mononuclear cells of inflammatory exudates. Biochem. Pharmacol., Suppl.,17, 185 (1968).
Furth, R. van, andZ. A. Cohn: The origin and kinetics of mononuclear phagocytes. J. exp. Med.128, 415 (1968).
Leder, L. D.: Der Blutmonozyt. Berlin-Heidelberg-New York: Springer 1967.
Schmalzl, F., u.H. Braunsteiner: Zytochemische Darstellung von Esteraseaktivität in Blut- und Knochenmarkszellen. Klin. Wschr.46, 642 (1968).
On the origin of monocytes. Acta haemat. (Basel)39, 177 (1968).
Shnitka, F. K., andA. M. Seligman: Role of esteratic inhibition on localization of esterase and the simultaneous cytochemical demonstration of inhibitor sensitive and resistant enzyme activity. J. Histochem. Cytochem.9, 504 (1961).
Spector, W. G., M. N. Walters, andD. A. Willoughby: The origin of the mononuclear cells in inflammatory exudates induced by fibrinogen. J. Path. Bact.90, 181 (1965).
Volkman, A., andJ. L. Gowans: The origin of macrophages from bone marrow in the rat. Brit. J. exp. Path.46, 62 (1965).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Schmalzl, F., Huber, C. & Braunsteiner, H. Demonstration of Proliferating Monocytic Precursors by the Combination of Cytochemical and Autoradiographic Methods. Klin Wochenschr 47, 887–888 (1969). https://doi.org/10.1007/BF01879925
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01879925