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Isolation of the mitogenic components from phytohaemagglutinin

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Summary

From PHA-P a pure mitogen was isolated, using a combination of two separation procedures, cation exchange chromotography and gel filtration. The biological activity of this purified mitogen was about a hundredfold higher compared with the starting material.

Zusammenfassung

Durch die Kombination zweier Trennverfahren, der Kationenaustausch-Chromotographie und der Gelfiltration wurde, ausgehend von PHA-P ein reines Mitogen isoliert. Die biologische Aktivität des gereinigten Mitogens war hundertfach höher als die des Ausgangsmaterials.

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Authors and Affiliations

  1. Medizinische Universitätsklinik Köln-Lindenthal, Germany

    K. Schumacher, G. Alzer, H. Oerkermann & G. Uhlenbruck

Authors
  1. K. Schumacher
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  2. G. Alzer
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  3. H. Oerkermann
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  4. G. Uhlenbruck
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  5. U. Wehmer
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  6. A. Schopen
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Supported by SFB 68 of the Deutsche Forschungsgemeinschaft.

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Schumacher, K., Alzer, G., Oerkermann, H. et al. Isolation of the mitogenic components from phytohaemagglutinin. Klin Wochenschr 50, 713–714 (1972). https://doi.org/10.1007/BF01495532

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  • DOI: https://doi.org/10.1007/BF01495532

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