Skip to main content
SpringerLink
Log in

Gewebsspezifische Hemmung der Lymphocytenproliferation durch Milzextrakt (Lymphocytenchalon)

Tissue specific inhibition of lymphocyte proliferation by spleen extract (lymphocyte chalone)

  • Originalien
  • Published:
Klinische Wochenschrift Aims and scope Submit manuscript

Summary

Aqueous spleen extracts were purified using acetone precipitation, membrane filtration, affinity chromatography, and dialysis. These extracts were able to inhibit thymidine incorporation into lymphoid cells (MKT-CH and PHA-stimulated lymphocyte cultures). They did not influence non lymphoid tissues (melanoma cells Mel Ei 78 and Ehrlich ascites cells). The inhibition was reversible and the purified extracts were not cytotoxic. The extracts correspond to a chalone. Their importance for prevention of graft versus host reaction and for treatment of lymphoproliferative diseases is discussed.

Zusammenfassung

Wäßrige Extrakte aus Kalbsmilz wurden durch Azetonfällung, Membranfiltration, Affinitätschromatographie und Dialyse gereinigt. Diese Extrakte hemmten den Thymidineinbau in lymphoiden Zellen (MKT-CH und PHA-stimulierte Lymphocytenkulturen). Zellen nicht lymphatischen Gewebes (Melanomzellen Mel Ei 78 und Ehrlich-Ascites-Zellen) wurden nicht beeinflußt. Die Hemmung war reversibel, und die Reinextrakte wirkten nicht zytotoxisch. Die Extrakte entsprechen einem Chalon. Ihre mögliche Bedeutung für die Verhinderung der Graft versus host reaction und für die Behandlung lymphoproliferativer Erkrankungen wird diskutiert.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literatur

  1. Attallah, A.M., Sunshine, G.H., Hunt, C.V., Houck, I.C.: The specific and endogenous mitotic inhibitor of lymphocytes (chalone). Exp. Cell Res., im Druck (1975)

  2. Argyris, T.S.: Chalones and the control of normal, regenerative, and neoplastic growth of the skin. Am. Zoologist12, 137–149 (1972)

    Google Scholar 

  3. Bateman, A.E.: Cell specifity of chalone-type inhibitors of DNA synthesis released by blood leucocytes and erythrocytes. Cell Tiss. Kinet.7, 451–461 (1974)

    Google Scholar 

  4. Boldingh, W.H., Laurence, E.B.: Extraction, purification and preliminary characterisation of the epidermal chalone: A tissue specific mitotic inhibitor obtained from vertebrate skin. Europ. J. Biochem.5, 191–198 (1968)

    PubMed  Google Scholar 

  5. Boyum, A.: Separation of white blood cells. Nature204, 793 (1964)

    PubMed  Google Scholar 

  6. Bullough, W.S., Laurence, E.B.: The control of epidermal mitotic activity in the mouse. Proc. roy. Soc. B151, 517–536 (1960)

    Google Scholar 

  7. Bullough, W.S.: The control of mitotic activity in adult mammalian tissues. Biol. Rev.37, 307–342 (1962)

    PubMed  Google Scholar 

  8. Bullough, W.S., Laurence, E.B.: Mitotic control by internal secretion. The role of the chalone-adrenalin complex. Exp. Cell Res.33, 176–194 (1964)

    PubMed  Google Scholar 

  9. Bullough, W.S., Laurence, E.B.: Control of mitosis in rabbit V×2 epidermal tumour by means of the epidermal chalone. Europ. J. Cancer4, 587–594 (1968)

    Google Scholar 

  10. Bullough, W.S., Laurence, E.B.: The lymphocytic chalone and its antimitotic action on a mouse lymphoma in vitro. Europ. J. Cancer6, 525–531 (1970)

    Google Scholar 

  11. Bullough, W.S., Deol, I.V.R.: The pattern of tumour growth. Symp. Soc. Exp. Biol.25, 255–275 (1971)

    PubMed  Google Scholar 

  12. Chopra, D.P., Cherkas, L.A., Flaxman, B.A.: Identification and partial purification of chalone from human epidermis. Brit. J. Derm.90, 37 (1974)

    PubMed  Google Scholar 

  13. Elgjo, K., Hennings, H.: Epidermal mitotic rate and DNA synthesis after injection of water extracts made from mouse skin treated with actinomycine D. Two or more growth-regulating substances. Virchows-Arch. Abt. B7, 342–347 (1971)

    Google Scholar 

  14. Elgjo, K.: Epidermal chalone and cyclic AMP. An in vitro study. J. invest. Derm.64, 14–18 (1975)

    PubMed  Google Scholar 

  15. Ernström, U., Nordlind, K.: Inhibition of lymphocyte proliferation in vitro by a splenic factor. Acta path. microbiol. scand. Sect. A82, 445–449 (1974)

    Google Scholar 

  16. Florentin, I., Kiger, N., Mathe, G.: T-lymphocyte specifity of a lymphocyte-inhibiting factor (chalone) extracted from the thymus. Europ. J. Immunol.3, 624–627 (1973)

    Google Scholar 

  17. Frankfurt, O.S.: Epidermal chalone. Effect on cell cycle and on development of hyperplasia. Exp. Cell Res.64, 140–144 (1971)

    PubMed  Google Scholar 

  18. Garcia-Giralt, E., Morales, V.H., Lasalvia, E., Mathe, G., Suppression of graft versus host reaction by spleen extract. J. Immunol.109, 878–880 (1972)

    PubMed  Google Scholar 

  19. Garcia-Giralt, E., Lasalvia, E., Florentin, I., Mathe, G.: Evidence for a lymphoid chalone. Rev. Europ. Etudes Clin. et Biol.15, 1013–1015 (1970)

    Google Scholar 

  20. Garcia-Giralt, E., Morales, V.H., Bizzini, B., Lasalvia, E.: Prevention of graft versus host reaction by incubation of lymphoid cells with a splenic extract (not affecting the repopulation of the haemopietic tissue). Cell Tiss. Kinet.6, 567–571 (1973)

    Google Scholar 

  21. Garcia-Giralt, E., Macieiro-Coelho, A.: Lymphocyte recognition and effector mechanisms (Ed. Lindahl-Kiessling, K., Osubu, D.), 457 (1974)

  22. Goedecke, K., Rensing, L.: Regulation des Zellzyklus. Naturw. Rundschau27, 4–74 (1974)

    Google Scholar 

  23. Houck, I.C., Irausquin, H., Leikin, S.: Lymphocyte DNA synthesis inhibition. Science173, 1139–1141 (1971)

    PubMed  Google Scholar 

  24. Houck, I.C., Irausquin, H., Leikin, S.: The lymphocyte chalone. Clin. Proc. Child. Hosp.28, 130–137 (1972)

    Google Scholar 

  25. Houck, I.C., Attallah, A., Lilly, I.R.: Immunosuppressive properties of the lymphocyte chalone. Nature245, 148–150 (1973)

    PubMed  Google Scholar 

  26. Houck, I.C., Hennings, H.: Chalones — specific endogenous mitotic inhibitors. FEBS Letters32, 1, 1–8 (1973)

    PubMed  Google Scholar 

  27. Houck, I.C., Irausquin, H., Leikin, S.: Specific endogenous mitotic inhibitors of human lymphocyte transformation. Children's Hosp. Research Foundation, Wash. D.C. (1973)

    Google Scholar 

  28. Jones, J., Paraskova-Tschernozenska, E., Moorhead, I.F.: In vitro inhibition of DNA synthesis in human leukaemic cells by a lymphoid cell extract. Lancet, 28, März, 654–655 (1970)

    Google Scholar 

  29. Kaltenbach, J.P., Kaltenbach, M.H., Lyons, W.B.: Nigrosin as a dye for differentiating live and dead ascites cells. Exp. Cell Res.15, 112–117 (1958)

    PubMed  Google Scholar 

  30. Kiger, N.: Isolation and immunological study of thymic lymphocytic inhibitory factors. Rev. Europ. Etudes Clin. Biol.16, 566–572 (1971)

    Google Scholar 

  31. Kiger, N., Florentin, I., Garcia-Giralt, E., Mathe, G.: Lymphocyte inhibitory factors (chalones) extracted from lymphoid organs. Extraction, partial purification and immunosuppressive properties. Transplantation Proceedings4, 531–535 (1972)

    PubMed  Google Scholar 

  32. Kiger, N., Florentin, I., Mathe, G.: Some effects of a partially purified lymphocyte inhibiting factor from calf thymus. Transplantation14, 448–453 (1972)

    PubMed  Google Scholar 

  33. Kiger, N., Florentin, I., Garcia-Giralt, E., Mathe, G.: Exp. Hemat.1, 22–28 (1973)

    PubMed  Google Scholar 

  34. Kiger, N., Florentin, I., Mathe, G.: Inhibition of graft versus host reaction by preincubation of the graft with a thymic extract (lymphocyte chalone). Transplantation16, 393–397 (1973)

    PubMed  Google Scholar 

  35. Laerum, O.D., Maurer, H.R.: Proliferation kinetics of myelopoietic cells and macrophages in diffusion chambers after treatment with granulocyte extracts (chalone). Virchows Arch. Abt. B14, 293–305. (1973)

    Google Scholar 

  36. Lasalvia, E., Garcia-Giralt, E., Macieira-Coelho, A.: Extraction of an inhibitor of DNA synthesis from human peripheral blood lymphocytes and bovine spleen. Europ. J. Clin. Biol. Res.15, 789 (1970)

    Google Scholar 

  37. Lord, B., Cercek, L., Cercek, B., Shah, G.P., Dexter, T.M., Lajtha, L.G.: Inhibitors of haemopoietic cell proliferation: Specificity of action within the haemopoietic system. Brit. J. Cancer29, 168–175 (1974)

    PubMed  Google Scholar 

  38. Mathe, G.: Lymphocyte inhibitors fulfilling the definition of chalones and immunosuppression. Rev. Europ. Etudes Clin. Biol.17, 548–551 (1972)

    Google Scholar 

  39. Mathe, G., Kiger, N., Florentin, I., Garcia-Giralt, E., Martyre, M.C., Halle-Pannenko, O., Schwartzenberg, L.: Progress in the prevention of GVH: Bone marrow grafts after ALG conditioning, with lymphocyte split chimerism, use of a lymphocyte „chalone T“, and soluble histocompatibility antigens. Transplantation Proceedings5, 933–939 (1973)

    PubMed  Google Scholar 

  40. Maurer, H.R.: Disc Electrophoresis. S. 72 (1971)

  41. Maurer, H.R.: Chalone: Regulationsstoffe des Organwachstums bei Mensch und Tier. Pharmazie in unserer Zeit2, 6, 181–189 (1973)

    Google Scholar 

  42. Metcalf, D.: Humoral regulators in the development and progression of leukaemia. Advanc. Cancer Res.14, 190 (1971)

    Google Scholar 

  43. Menzies, R.A., Kerrigan, I.M.: Liver extract antimitotic activity assayed with chick embryos. Exp. Cell Res.86, 430 (1974)

    PubMed  Google Scholar 

  44. Moorhead, I.F., Paraskova-Tschernozenska, E. Pirrie, A.I., Hayes, C.: Lymphoid inhibitor of human lymphocyte DNA synthesis and mitosis in vitro. Nature224, 1207–1208 (1969)

    PubMed  Google Scholar 

  45. Morales, V.H., Garcia-Giralt, E.: Immunosuppressive action in vitro by a bovine spleen extract. Z. Naturforsch.26b, 1339–1340 (1971)

    Google Scholar 

  46. Nowell, P.C.: Phytohemagglutinin: an initiator of mitosis in cultures of normal human leucocytes. Cancer Res.20, 462 (1960)

    PubMed  Google Scholar 

  47. Paukovits, W.R., Paukovits, I.B.: Mechanism of action of granulopoiesis inhibiting factor (chalone). I. Evidence for a receptor protein on bone marrow cells. Exp. Pathol. im Druck (1975)

  48. Rohrbach, R., Laerum, O.D.: Variations of mitosis inhibiting chalone activity in epidermis and dermis after carcinogen treatment. Cell Tiss. Kinet.7, 251–257 (1974)

    Google Scholar 

  49. Rytömaa, T., Kiviniemi, K.: Regression of generalized leukaemia in rat induced by the granulocyte chalone. Europ. J. Cancer6, 401–410 (1970)

    Google Scholar 

  50. Rytömaa, T., Kiviniemi, K.: Control of DNA duplication in rat chloroleukaemia by means of the granulocyte chalone. Europ. J. Cancer4, 595–606 (1968)

    Google Scholar 

  51. Saal, J.G.,et al.: in Vorbereitung, (1975)

  52. Shafer, E.A.: The Endocrine Organs. Longmans, Green&Co., London. (1916)

    Google Scholar 

  53. Simard, A., Corneille, L., Deschamps, Y., Verly, W.G.: Inhibition of cell proliferation in the livers of hepatectomized rats by a rabbit hepatic chalone. Proc. Nat. Acad. Sci. (Wash.)71, 1763–1766 (1974)

    PubMed  Google Scholar 

  54. Volm, M., Kinzel, V., Mohr, U., Süss, R.: Inactivation of tissue-specific inhibitors by a carcinogen (diethylnitrosamine). Experientia25, 68–69 (1969)

    PubMed  Google Scholar 

  55. Weiss, P., Kavanau, I.L.: A model of growth and growth control in mathematical terms. J. Gen. Physiol.41, 1–47 (1957)

    PubMed  Google Scholar 

  56. Wilms, K., Wilmanns, W.: Erythrocytes, Thrombocytes, Leucocytes. (Ed. Gerlach, E., Moser, K., Deutsch, E., Wilmanns, W.), Thieme Verlag, S. 483–484 (1972)

  57. Wilms, K.: DNS-Synthese in menschlichen Blutzellen, Thieme Copythek, S. 55–60 (1975)

  58. Zacharius, R.M., Zell, T.E., Morrison, J.H., Woodlock, J.J.: Glycoprotein staining following electrophoresis on acrylamide gels. Analyt., Biochem.30, 148–152 (1969)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Abt. Innere Medizin II, Medizinische Universitätsklinik Tübingen, Germany

    Else Heidemann, A. Jung & K. Wilms

  2. Physiologisch-Chemisches Institut der Universität Tübingen, Germany

    Else Heidemann & A. Jung

Authors
  1. Else Heidemann
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Jung
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. Wilms
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Die Untersuchungen wurden durch ein Stipendium der Deutschen Forschungsgemeinschaft unterstützt.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Heidemann, E., Jung, A. & Wilms, K. Gewebsspezifische Hemmung der Lymphocytenproliferation durch Milzextrakt (Lymphocytenchalon). Klin Wochenschr 54, 221–226 (1976). https://doi.org/10.1007/BF01469129

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01469129

Key words

Schlüsselwörter

Search

Navigation