Zusammenfassung
Bei 6 Patienten mit Cushing-Syndrom fand sich eine 3–4fach gesteigerte Aktivität der Na-K-ATPase in Erythrocytenmembranen im Vergleich zu 28 Kontrollpersonen (0.986±0.291 gegenüber 0.259±0.1 µM Pi·h−1·mg−1,p<0.001). Die Strophan-thininsensible Mg-ATPase war bei beiden Gruppen gleich. Der Befund spricht für eine Aktivierung der Natriumpumpe an den Zellmembranen von Patienten mit Glukokortikoidexceß.
Summary
The Na-K-ATPase activity of erythrocyte ghosts was increased in 6 patients with Cushing's syndrome compared with 28 control subjects (0.986±0.291 versus 0.259±0.1 µM Pi·h−1·mg−1,p<0.001). Ouabain insensitive Mg-ATPase activity was similar in both groups. These data support the concept of an activation of the Na-pump in patients with glucocorticoid excess.
This is a preview of subscription content, log in via an institution to check access.
References
Charney AN, Kinsey MD, Myers L, Giannella RA, Gots RE (1975) Na-K-activated Adenosine-Triphosphatase and Intestinal Electrolyte Transport. J Clin Invest 56:653–660
Charney AN, Silva P, Besarab A, Epstein FH (1974) Separate effects of aldosterone, DOCA and methylprednisolone on renal Na-K-ATPase. Am J Physiol 227:345–350
De Santo NG, Ebel H, Hierholzer K (1971) Plasma cell Membranes of Rat Kidney II ATPase Activities in Adrenalectomized Rats with and without Steroid Hormone Substitution. Pflügers Arch 324:26–42
Fiske CH, Subbarow Y (1925) The colorimetric determination of phosphorus. J Biol Chem 66:375–399
Haack D, Möhring JI, Möhring B, Petri M, Hackenthal E (1977) Comparative study on development of corticosterone and DOCA hypertension in rats. Am J Physiol 232:F 403–411
Ismail-Beigi F, Edelman IS (1973) Effects of thyroid status on electrolyte distribution in rat tissues. Am J Physiol 225:1172–1177
Jørgensen PL (1968) Regulation of the Na-K-activated ATP Hydrolyzing Enzyme System in rat kidney. I. The Effect of Adrenalectomy and the supply of sodium on the Enzyme System. Biochem Biophys Acta 151:212–224
Jørgensen PL, Hansen O, Glynn IM, Cavieres ID (1973) Antibodies to pig kidney Na-K-ATPase inhibit the Na-pump in human red cells provided they have access to the inner surface of the cell membrane. Biochem Biophys Acta 291:795–800
Katz AI, Epstein FH (1967) The Role of Sodium-Potassium-Activated Adenosine-Triphosphatase in the Reabsorption of Sodium by the Kidney. J Clin Invest 46:1999–2011
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275
Massa EM, Morero RD, Bloj B, Farias RN (1975) Hormone action and membrane Fluidity: Effect of Insulin and Cortisol on the Hill coefficients of Rat Erythrocyte membrane-bound Acetylcholinesterase and Na-K-ATPase. Biochem Biophys Res Comm 66:115–122
Post RL, Merritt CR, Kinsolving CR, Albright CD (1960) Membrane adenosine triphosphatase as a participant in the active transport of sodium and potassium in the human erythrocyte. J Biol Chem 235:1796–1802
Schmidt U, Dubach UC (1971) Sensitivity of Na-K-Adenosine Triphosphatase Activity in various Structures of the Rat Nephron: Studies with Adrenalectomy. Europ J Clin Invest 1:307–312
Swingle WW, Brannick I, Osborn M, Glenister D (1957) Effect of gluco- and mineralocorticoid adrenal steroids on fluid and electrolytes of fasted adrenalectomized dogs. Proc Soc Exptl Biol Med 96:446–452
Wambach G, Helber A, Bönner G, Hummerich W (1979) Na-K-ATPase Aktivität in Erythrocytenghost von Patienten mit essentieller Hypertonie. Klin Wochenschr 57:169–172
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wambach, G., Helber, A., Allolio, B. et al. Increased activity of the Na-K-ATPase in red cell-ghosts of patients with Cushing's Syndrome: Possible significance for the pathogenesis of glucocorticoid-induced hypertension. Klin Wochenschr 58, 485–487 (1980). https://doi.org/10.1007/BF01476805
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01476805