Summary
An attempt is made to evaluate those portions of low molecular hydrophilic solutes that obey apparent passive transfer principles at the blood cerebrospinal fluid (CSF) barrier under steady state conditions. Twenty-four electrolytes, metabolites, and amino acids are determined in serum and CSF obtained simultaneously. The correlation found between the serum/CSF concentration ratios and the molecular radii of most compounds is indicative of the prevalence of passive transfer processes in CSF formation, as has been already demonstrated for proteins. The level of some constituents in the primary filtrate may be secondarily increased by influx from the adjacent extracellular space, e.g. glutamine and creatinine, or decreased by specific uptake from the CSF compartment, e.g. glycine. The CSF levels of all compounds are dependent upon the actual state of the blood CSF barrier as evaluated by the serum/CSF concentration ratios of albumin and α2-macroglobulin. The results do not support the assumption that CSF may be used to test the permeability state of the blood brain barrier propery.
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References
Aprison MH, Nadi NS (1978) Glycine: Inhibition from the sacrum to the medulla. In: Fonnum F (ed). Plenum Press, New York, pp 531–570
Bito LZ, Davson H, Fenstermacher JD (1978) The ocular and cerebrospinal fluids. Academic Press, London
Bohuon C (1962) Microdosage du magnesium dans divers milieux biologiques. Clin Chim Acta 7:811–817
Bradbury M (1979) The concept of a blood-brain-barrier. Wiley and Sons, Chichester
Bradbury MWB, Stubbs J, Hughes IE, Parker P (1963) The distribution of potassium, sodium chloride and urea between lumbar cerebrospinal fluid and blood serum in human subjects. Clin Sci 25:97–105
Bradford HF, de Belleroche JS, Ward HK (1978) On the metabolic and intrasynaptic origin of amino acid transmitters. In: Fonnum F (ed). Plenum Press, New York, pp 367–377
Brightman MW (1965) The distribution within the brain of ferritin injected into the cerebrospinal fluid compartment. Am J Anat 117:193–219
Cohen H (1924) The inorganic phosphorus content of the cerebrospinal fluid. Q J Med 17:289–301
Cotman CW, Hamberger A (1978) Glutamate as a CNS neurotransmitter: Properties of release, inactivation and biosynthesis. In: Fonnum F (ed). Plenum Press, New York, pp 379–412
Cremer JE, Sarna GS, Teal HM, Cunningham VJ (1978) Amino acid precursors: their transport into brain and initial metabolism. In: Fonnum F (ed). Plenum Press, New York, pp 669–689
Crone C (1965) Facilitated transfer of glucose from blood into brain tissue. J Physiol 181:103–113
Cserr HE (1971) Physiology of the choroid plexus. Physiol Rev 51:273–311
Davson H (1972) The blood-brain-barrier. In: Bourne GH (ed) The structure and function of nervous tissue, vol IV. Academic Press, New York, pp 321–445
Dörner K (1975) Fluorimetrische Calciumbestimmung durch EGTA-Titration. Ärztl Lab 21:48–57
Evans CAN, Reynolds JM, Reynolds ML, Saunders NR, Segal MB (1974) The development of a blood-brain-barrier mechanism in foetal sheep. J Physiol 238:371–386
Felgenhauer K (1974) Protein size and cerebrospinal fluid composition. Klin Wochenschr 52:1158–1164
Felgenhauer K (1980) Protein filtration and secretion at human body fluid barriers. Pflügers Arch 384:9–17
Fenstermacher JD, Patlak CS (1975) The exchange of material between cerebrospinal fluid and brain. In: Cserr HF et al. (eds) Fluid environment of the brain. Academic Press, New York, pp 201–213
Ferguson RK, Woodbury DM (1969) Penetration of14C-Inulin and14C-Sucrose into brain, cerebrospinal fluid and skeletal muscle of developing rats. Exp Brain Res 7:181–194
Fischer-Williams M (1976) Recent advances in the study of cerebrospinal fluid. Practitioner 217:108–115
Fonnum F (1978) Amino acids as chemical transmitters. Plenum Press, New York
Forster RE (1971) The transport of water in erythrocytes. In: Bronner F, Kleinzeller A (eds) Current topics in membranes and transport, vol II. Academic Press, New York, pp 91–121
Gindler EM, Ishizaki RT (1969) Rapid seminicro colorimetric determination of phosphorus in serum and nonionic surfactants. Clin Chem 15:807–813
Greenstein JP, Winitz M (1961) Chemistry of the Amino Acids, vol I. Wiley, New York
Hamberger A, Sellström Å, Weiler ChT (1978) Glial cells and amino acid transmitters. In: Fonnum F (ed). Plenum Press, New York, pp 653–662
Hamilton PB (1963) Ion exchange chromatography of amino acids. A single column, high resolving, fully automated procedure. Anal Chem 35:2055–2062
Hille B (1975) Ionic selectivity of Na and K channels of nerve membranes. In: Eisenmann G (ed) Membranes, vol III. Dekker, New York, pp 255–323
Höfer M (1977) Transport durch biologische Membranen. Verlag Chemie, Weinheim New York
Horak E, Sunderman jr FW (1972) Measurements of serum urea nitrogen by a conductivimetric urease assay. Ann Clin Lab Sci 2:425–430
Iversen LL, Dick F, Kelly JS, Schon F (1975) Uptake and localisation of transmitter amino acids in the nervous system. In: Berl S, Clarke DD, Schneider D (eds) Metabolic compartmentation and neurotransmission. Plenum Press, New York
Jost W (1964) Fundamental aspects of diffusion processes. Angew Chemie 3:713–718
Katzmann R (1976) Blood-Brain-CSF-Barriers. In: Siegel GJ et al. (eds) Basic neurochemistry. Little, Brown and Co, Boston, pp 414–428
Landis EM, Pappenheimer JR (1963) Exchange of substances through capillary walls. In: Hamilton WF, Dow P (eds) Handbook of physiology, vol 2. Williams and Wilkins, Baltimore, pp 961–1034
Lee JC (1971) Evaluation in the concept of the blood-brain-barrier function. Prog Neuropathol 1:84–145
Levin E, Nogneira GJ, Argiz CAG (1966) Ventriculo-cisternal perfusion of amino acids in cat brain. J Neurochem 13:761–767
Liappis N, Jäkel A, Bantzer P (1977) Verhalten der freien Aminosäuren im Liquor cerebrospinalis von Kindern. Klin Paediatr 189:155–160
Maker HS, Clarke DD, Lajtha AL (1976) Intermediary metabolism of carbohydrates and amino acids. In: Siegel GJ et al. (eds) Basic neurochemistry. Little, Brown and Co, Boston, pp 279–307
Mayer St, Maichel RR, Brodie BB (1959) Kinetics of penetration of drugs and other foreign compounds into cerebrospinal fluid and brain. J Pharmacol 127:205–211
McGale EHF, Peye IF, Stonier C, Hutchinson EC, Aber GM (1977) Studies of the inter-relationship between cerebrospinal fluid and plasma amino acid concentration in normal individuals. J Neurochem 29:291–297
Oldendorf WH (1971) Brain uptake of radio labeled amino acids, amines, and hexoses after arterial injection. Am J Physiol 221:1629–1639
Perry TL, Hansen S, Kennedy J (1975) CSF amino acids and plasma-CSF amino acid ratio in adults. J Neurochem 24:587–589
Plenert W, Heine W (1973) Normalwerte. Verlag Volk und Gesundheit, Berlin
Rall DP, Oppelt WW, Patlak CS (1962) Extracellular space of brain as determined by diffusion of inulin from the ventricular system. Life Sci 2:43–48
Rapoport StJ (1976) Blood-brain-barrier in physiology and medicine. Raven Press, New York
Rauen HM (1964) Biochemisches Taschenbuch. Springer, Berlin Göttingen Heidelberg New York
Renkin EM (1955) Filtration, diffusion and molecular sieving through porous cellulose membranes. J Gen Physiol 38:225–243
Schliep G, Felgenhauer K (1978) Serum-CSF protein gradients, the blood-CSF barrier and the local immune response. J Neurol 218:77–96
Stevens JF (1971) Determination of glucose by an automatic analyzer. Clin Chim Acta 32:199–205
Yudilevich DL, De Rose N, Sepulveda FV (1972) Facilitated transport of amino acids through the blood-brain-barrier of the dog studied in a single capillary circulation. Brain Res 44:569–578
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Felgenhauer, K., Liappis, N. & Nekic, M. Low molecular solutes and the blood cerebrospinal fluid barrier. Klin Wochenschr 60, 1385–1392 (1982). https://doi.org/10.1007/BF01716243
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DOI: https://doi.org/10.1007/BF01716243