Summary
Interspecific hybrids and amphidiploids of Nicotiana knightiana Goodspeed (n= 12)x N. umbratica Burbidge (n = 23) resembled either parent in some characters and were intermediate in other characters. The F1 hybrids (2n = 35) showed mostly univalents during meiosis, while the amphidiploids (2n = 70) formed bivalents almost regularly. The former were completely sterile and the latter fully male fertile but predominantly female sterile. This female sterility was due to disintegration of the embryo sacs leading to collapsed ovules. The few fertile ovules, however, showed normal development of embryo sac and embryo. The occurrence of fertile and sterile ovules was believed to be due to segregation of the genes governing sterility.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Greenleaf WA (1941) Sterile and fertile amphidiploids. Their possible relation to the origin of Nicotiana tabacum. Genetics 26:301–324
Johansen DA (1940) Plant microtechnique. McGraw Hill Publ, New York
Miller RA, Gumborg OL, Keller WA, Kao KN (1971) Fusion and division of nuclei in multinucleated soybean protoplasts. Can J Genet Cytol 13:347–353
Murthy UR (1971) A standardised cotton blue stain for pollen germination and growth in Andropogoneae grasses. Stain Technol 46:239–243
Author information
Authors and Affiliations
Additional information
Communicated by R. Hagemann
Rights and permissions
About this article
Cite this article
Gangadevi, T., Rao, P.N., Rao, B.H. et al. A study of morphology, cytology and sterility in interspecific hybrids and amphidiploids of Nicotiana knightiana X N. umbratica . Theoret. Appl. Genetics 70, 330–332 (1985). https://doi.org/10.1007/BF00304921
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00304921