Summary
Theoretical flow karyotypes from both plant and mammalian species have been simply modelled using computer spreadsheet software. The models are based upon published values of relative DNA content or relative lengths of each of the chromosomes. From such data, the histograms of chromosome distribution have been simulated for both linear and logarithmic modules of a flow cytometer, and as a function of the coefficient of variation. Simulated and experimental histograms are compard for Nicotiana plumbaginifolia. This readily accessible exercise facilitates the planning and execution of flow cytometric analysis and sorting of chromosomes.
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References
Árnason Ú (1974) Comparative chromosome studies in Cetacea. Hereditas 77:1–36
Bennett MD, Heslop-Harrison JS, Smith JB, Ward JP (1983) DNA density in mitotic and meiotic metaphase chromosomes of plants and animals. J Cell Sci 63:173–179
Bosemark NO, Bormotov VE (1971) Chromosome morphology in a homozygous line of sugar beet. Hereditas 69:205–212
Carrano AV, Lebo RV, Yu LC, Kan YW (1981) Regional gene mapping of human chromosomes purified by flow sorting. In: Neth R, Gallo RC, Graf T (eds) Modern trends in human leukemia IV. Springer, Berlin Heidelberg New York, pp156–159
Conia J, Muller P (1988) Isolation, classification and flow cytometric sorting of plant chromosomes. In: Yen A (ed) Flow cytometry: Advanced experimental and clinical applications. CRC Press, Boca Raton/FL, USA (in press)
Conia J, Bergounioux C, Perennes C, Muller P, Brown S, Gadal P (1987) Flow cytometric analysis and sorting of plant chromosomes from Petunia hybrida protoplasts. Cytometry 8:500–508
De Laat AMM, Blaas J (1984) Flow-cytometric characterization and sorting of plant chromosomes. Theor Appl Genet 67:463–467
Fox KR, Howarth NR (1985) Investigations into the sequenceselective binding of mithramycin and related ligands to DNA. Nucleic Acids Res 13:8695–8714
Gray JW, Langlois RG (1986) Chromosome classification and purification using flow cytometry and sorting. Annu Rev Biophys Chem 15:195–235
Gray JW, Carrano AV, Steinmetz LL, Van Dilla MA, Moore II DH, Mayall BH, Mendelsohn ML (1975) Chromosome measurement and sorting by flow systems. Proc Natl Acad Sci USA 72:1231–1234
Gray JW, Langlois RG, Carrano AV, Burkhart-Schulte K, Van Dilla MA (1979) High resolution chromosome analysis: One and two parameter flow cytometry. Chromosoma 73:9–27
Gray JW, Dean PN, Fuscoe JC, Peters DC, Trask BJ, Van den Engh GJ, Van Dilla MA (1987) High-speed chromosome sorting. Science 238:323–329
Grunwald D, Reuter W-O, Rosenfeld C, Frelat G (1983) Analyse de chromosomes humains par microcytophotomètre de flux. C R Acad Sci Ser III 297:299–304
Grunwald D, Geffrotin C, Chardon P, Frelat G, Vaiman M (1986) Swine chromosomes: Flow sorting and spot blod hybridization. Cytometry 7:582–588
Hansen KM (1975) The Gand Q-band karyotype of Böhm's or Grant's zebra (Equus burchelli böhmi). Hereditas 81:133–140
Jackson RC (1957) New low chromosome number for plants. Science 126:1115–1116
Kapuściński J, Szer W (1979) Interactions of 4′, 6-diamidine-2-phenylindole with synthetic polynucleotides. Nucleic Acids Res 6:3519–3534
Langlois RG, Yu LC, Gray JW, Carrano AV (1982) Quantitative karyotyping of human chromosomes by dual beam flow cytometry. Proc Natl Acad Sci USA 79:7876–7880
Lin CC, Biederman BM, Jamro HK, Hawthorne AB, Church RB (1980) Porcine (Susscrofa domestica) chromosome identification and suggested nomenclature. Can J Genet Cytol 22:103–116
Maizonnier D (1976) Etude cytogenetique de variations chromosomiques naturelles ou induites chez Petunia hybrida Hort. Thèse Doct ès Sci Nat Université de Dijon, France, pp1–98
Manolov G, Manolova Y, Levan A (1971) The fluorescence pattern of the human karyotype. Hereditas 69:273–286
Mendelsohn ML, Mayall BH, Bogart E, Moore II DH, Perry BH (1973) DNA content and DNA-based centromeric index of the 24 human chromosomes. Science 179:1126–1129
Mori M, Sasaki M (1973) Fluorescence banding patterns of the rat chromosomes. Chromosoma 40:173–182
Mouras A, Wildenstein C, Salesses G (1986) Analysis of karyotype and C-banding pattern of Nicotiana plumbaginifolia using two techniques. Genetica 68:197–202
Muleris M, Paravatou-Petsota M, Dutrillaux B (1984) Diagrammatic representation for chromosomal mutagenesis studies II. Radiation-induced rearrangements in Macaco fascicularis. Mutat Res 126:93–103
Schnedl W (1971) The karyotype of the mouse. Chromosoma 35:111–116
Sheridan WF (1982) Maps, Markers and Stocks. In: Sheridan WF (ed) Maize for biological research. III. Genetics and Cytogenetics. A special publication of the Plant Molecular Biology Association. University of North Dakota, University Press, Charlottesville, pp 37–52
Sjödin J (1971) Induced translocation in Vicia faba L. Hereditas 68:1–34
Southern EM (1982) Application of DNA analysis to mapping the human genome. Cytogenet Cell Genet 32:52–57
Van den Engh G, Trask B, Cram S, Bartholdi M (1984) Preparation of chromosome suspensions for flow cytometry. Cytometry 5:108–117
Weisblum B, Haenssler E (1974) Fluorometric properties of the bibenzimidazole derivative Hoechst 33258, a fluorescent probe specific for AT concentration in chromosomal DNA. Chromosoma 46:255–260
Young, BD, Ferguson-Smith MA, Sillar R, Boyd E (1981) Highresolution analysis of human peripheral lymphocyte chromosomes by flow cytometry. Proc Natl Acad Sci USA 78:7727–7731
Yu L-C, Aten J, Gray J, Carrano AV (1981) Human chromosome isolation from short-term lymphocyte culture for flow cytometry. Nature 293:154–155
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Communicated by F. Mechelke
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Conia, J., Muller, P., Brown, S. et al. Monoparametric models of flow cytometric karyotypes with spreadsheet software. Theoret. Appl. Genetics 77, 295–303 (1989). https://doi.org/10.1007/BF00266200
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DOI: https://doi.org/10.1007/BF00266200