Abstract
Enzyme electrophoresis was used to compare the isozyme phenotypes of Oryza sativa, IR31917 (AA genome), and two O. minuta accessions (Om 101089 and Om101141; BBCC genome) for ten enzyme systems. Between the two species, two systems were monomorphic (isocitrate dehydrogenase and alcohol dehydrogenase) and eight were polymorphic (shikimate dehydrogenase, phosphogluconate dehydrogenase, phosphoglucose isomerase, malate dehydrogenase, glutamate oxaloacetate transaminase, esterase, aminopeptidase, and endopeptidase). Polymorphism between O. minuta accessions was detected for shikimate dehydrogenase and glutamate oxaloacetate. As expected, the quaternary structure of the O. minuta isozymes was comparable to that of O. sativa. Possible allelic relationships with known O. sativa alleles and their genomic designation are discussed. Combined with chromosome data, the interspecific variation was exploited to monitor the relative genetic contribution of the two parents in the IR31917/Om101141 F1 hybrids and recurrent (IR31917) backcross progenies. The isozyme content of F1 hybrid reflected its triploid nature (ABC genome composition), while that of the backcross progenies paralleled the duplication of the A genome and the gradual loss of O. minuta chromosomes during the backcrossing process. Evidence is provided for a degree of homoeology between the A, B, and C genomes, and for introgression from O. minuta into O. sativa.
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Amante-Bordeos A, Sitch LA, Nelson R, Dalmacio RD, Oliva NP, Aswidinnoor H, Leung H (1992) Transfer of bacterial blight and blast resistance from the tetraploid wild rice Oryza minuta to cultivated rice, Oryza sativa. Theor Appl Genet 84:345–354
Bergman JW, Maan SS (1973) Genetic control of isozymes in wheat-rye addition lines with rye or wheat cytoplasm. In: Sears ER, Sears LM (eds) Proc 4th Int Wheat Genetics Symp, University of Missouri, Columbia, pp 329–335
Chen BY, Heneen WK, Simonsen V (1989) Comparative and genetic studies of isozymes in resynthesized and cultivated Brassica napus L., B. campestris L. and B. alboglabra Bailey. Theor Appl Genet 77:673–679
Endo T, Morishima H (1983) Current status of isozyme research in individual plant species. 6. Rice. In: Tanksley SD, Orton JJ (eds) Isozymes in plant genetics and breeding. Part A. Elsevier Science Publishers B.V., Amsterdam, pp 129–146
Gale MD, Miller TE (1987) The introduction of alien genetic variation in wheat. In: Lupton FGH (ed) Wheat breeding. Its scientific basis. Chapman and Hall, London New York, pp 173–210
Glaszmann JC (1986) A varietal classification of Asian cultivated rice (Oryza sativa L.) based on isozyme polymorphism. In: Rice genetics. International Rice Research Institute, Manila, Philippines, pp 83–90
Glaszmann JC, De los Reyes BG, Khush GS (1988) Electrophoretic variation of isozymes in plumules of rice (Oryza sativa L.) — a key to the identification of 76 alleles at 24 loci. Int Rice Res Inst Res Pap Ser No 134
Hart GE, Tuleen NA (1983a) Introduction and characterization of alien genetic material. In: Tanksley SD, Orton JJ (eds) Isozymes in plant genetics and breeding. Part A. Elsevier Science Publishers B.V., Amsterdam, pp 339–362
Hart GE, Tuleen NA (1983b) Chromosomal locations of eleven Elytrigia elongata (= Agropyron elongatum) isozyme structural genes. Genet Res 41:181–202
Hart GE, Islam AKMR, Shepherd KW (1980) Use of isozymes as chromosome markers in the isolation and characterization of wheat-barley chromosome addition lines. Genet Res 36:311–325
IRRI (1990) Program Report for 1989. International Rice Research Institute, Manila, p 144
Ishikawa R (1991) Integration of isozyme genes on chromosome 12 into a linkage map. Rice Genet Newslett 8:100–103
Jena KK, Khush GS (1989) Monosomic alien addition lines of rice: production, morphology, cytology, and breeding behavior. Genome 30:1–16
Khush GS (1977) Disease and insect resistance in rice. Adv Agron 29:265–341
Khus GS, Multani DS, de los Reyes BG, Brar DS (1991) Chromosomal location of Mdh-3 and Pgd-2 loci in rice through monosomic alien addition line (MAAL) analysis. Rice Genet Newslett 8:106–109
Li HW, Weng TS, Chen CC, Wang WH (1962) Cytogenetical studies of Oryza sativa L. and its related species. 2. A preliminary note on the interspecific hybrids within the Section Sativa Roschev. Bot Bull Acad Sinica 3:209–219
Markert CL, Whitt GS (1968) Molecular varieties of isozymes. Experimentia 24(10):977–1088
McCouch SR, Kochert G, Yu ZH, Wang Y, Khush GS, Coffman WR, Tanksley SD (1988) Molecular mapping of rice chromosomes. Theor Appl Genet 76:815–829
Nakagahra M, Akihama T, Hayashi KI (1975) Genetic variation and geographic cline of esterase isozymes in nature rice varieties. Jpn J Genet 50:373–380
Peffley EB, Corgan JN, Horak KE, Tanksley SD (1985) Electrophoretic analysis of Allium alien addition lines. Theor Appl Genet 71:176–184
Ranjhan S, Glaszmann JC, Ramirez DA, Khush GS (1988) Chromosomal location of four isozyme loci by trisomic analysis in rice (Oryza sativa L.) Theor Appl Genet 75(4):541–545
Rick CM (1969) Controlled introgression of chromosomes of Solanum pennellii into Lycopersicon esculentum: segregation and recombination. Genetics 62:753–768
Sano R, Morishima H (1984) Linkage relationships among 6 polymorphic isozyme loci in rice cultivars. Jpn J Breed 34(suppl 1):252–253
Second G (1982) Origin of the genetic diversity of cultivated rice (Oryza spp.): study of the polymorphism scored at 40 isozyme loci. Jpn J Genet 57:25–27
Shahi BB, Morishima H, Oka HI (1969) A survey of variations in peroxidase, acid phosphatase and esterase isozymes of wild and cultivated Oryza species. Jpn J Genet 44:303–319
Shaw C, Prasad R (1970) Starch-gel electrophoresis of enzymes, a compilation of recipes. Indian J Genet Breed 20:15–21
Shields CR, Orton TJ, Stuber CW (1983) An outline of general resource needs and procedures for the electrophoretic separation of active enzymes from plant tissue. In: Tanksley SD, Orton TJ (eds) Isozymes in plant genetics and breeding, Part A, Elsevier Science Publishers BV, Amsterdam, pp 443–468
Sitch LA (1990) Incompatibility barriers operating in crosses of Oryza sativa with related species and genera. In: Gustafson JP (ed) Genetic manipulation in plant improvement II. Plenum Press, New York, pp 77–94
Sitch LA, Amante AD, Dalmacio RD, Leung H (1989) Oryza minuta, a source of disease resistance for rice improvement. In: Mujeeb-Kazi A, Sitch LA (eds) Review of advances in plant biotechnology, 1985–1988. International Maize and Wheat Improvement Center, Mexico, and International Rice Research Institute, Manila, pp 315–322
Stephens SG (1949) The cytogenetics of speciation in Gossypium. I. Selective elimination of the donor parent genome in interspecific backcrosses. Genetics 34:627–637
Tanksley SD, Rick CM (1980) Isozyme gene linkage map of the tomato: applications in genetics and breeding. Theor Appl Genet 57:161–170
Tang KS, Hart GE (1975) Use of isozymes as chromosome markers in wheat-rye addition lines and in triticale. Genet Res 26:187–201
Wu KS, Glaszmann JC, Khush GS (1988) Chromosomal location of ten isozyme loci in rice (Oryza sativa L.) through trisomic analysis. Biochemical Genetics 26:303–320
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Communicated by J. W. Snape
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Romero, G.O., Amante-Bordeos, A.D., Dalmacio, R.D. et al. Comparative studies of isozymes in Oryza sativa, O. minuta, and their interspecific derivatives: evidence for homoeology and recombination. Theoret. Appl. Genetics 87, 609–615 (1993). https://doi.org/10.1007/BF00221886
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DOI: https://doi.org/10.1007/BF00221886