Abstract
Analyses of RFLPs, isozymes, morphological markers and chromosome pairing were used to isolate 12 Triticum aestivum cv Chinese Spring (genomes A, B, and D)-T. peregrinum (genomes Sv and Uv) disomic chromosome addition lines. The evidence obtained indicates that each of the 12 lines contains an intact pair of T. peregrinum chromosomes. One monosomic addition line, believed to contain an intact 6Sv chromosome, was also isolated. A CS-7Uv chromosome addition line was not obtained. Syntenic relationships in common with the standard Triticeae arrangement were found for five of the seven Sv genome chromosomes. The exceptions were 4Sv and 7Sv. A reciprocal translocation exists between 4S1 and 7S1 in T. longissimum and evidence was obtained that the same translocation exists in T. peregrinum. In contrast, evidence for syntenic relationships in common with the standard Triticeae arrangements were found for only one Uv chromosome of T. peregrinum.; namely, chromosome 2Uv. All other Uv genome chromosomes are involved in at least one translocation, and the same translocations were found in the U genome of T. umbellulatum. Evidence was also obtained indicating that the centromeric regions of 4U and 4Uv are homoeologous to the centromeric regions of Triticeae homoeologous group-6 chromosomes, that the centromeric regions of 6U and 6Uv are homoeologous to the centromeric regions of group-4 chromosomes, and that 4U and 4Uv are more closely related overall to Triticeae homoeologous group-6 chromosomes than they are to group-4 chromosomes.
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Anderson JA, Ogihara Y, Sorrells ME, Tanksley SD (1992) Development of a chromosomal-arm map for wheat based on RFLP markers. Theor Appl Genet 83:1035–1043
Benito C, Gallego FJ, Frade JM, Zaragoza C, Figueiras AM (1990) Chromosomal location of adenylate kinase isozymes in Triticeae species. Theor Appl Genet 79:157–160
Chen Z, Devey M, Tuleen NA, Hart GE (1994) Use of recombinant substitution lines in the construction of RFLP-based genetic maps of chromosomes 6A and 6B of tetraploid wheat (Triticum turgidum L.). Theor Appl Genet 89:703–712
Chenicek KJ, Hart GE (1987) Identification and chromosomal locations of aconitase gene loci in Triticeae species. Theor Appl Genet 74:261–268
Devey M, Hart GE (1993) Chromosomal localization of intergenomic RFLP loci in hexaploid wheat. Genome 36:913–918
Devos KM, Atkinson MD, Chinoy CN, Francis HA, Harcourt RL, Koebner RMD, Liu CJ, Masojc P, Xie DX, Gale MD (1993) Chromosomal rearrangements in the rye genome relative to that of wheat. Theor Appl Genet 85:673–680
Dvorak J (1980) Homoeology between Agropyron elongatum chromosomes and Triticum aestivum chromosomes. Can J Genet Cytol 22:237–259
Feldman M (1975) Alien addition lines of common wheat containing Triticum longissimum chromosomes. Proc 12th Int Bot Congr 2:506
Feldman M (1977) Historical aspects and significance of the discovery of wild wheats. Stadler Genet Symp 9:121–144
Feldman M (1979a) New evidence on the origin of the B genome of wheat. In: Proc 5th Int Wheat Genet Symp, New Delhi, India. Vol 1, pp 120–132
Feldman M (1979b) Genetic resources of wild wheat and their use in breeding. Monografia Genet Agraria 4:9–26
Feldman M, Sears ER (1981) The wild gene resources of wheat. Sci American 244:102–112
Friebe M, Tuleen N, Jiang J, Gill BS (1993) Standard karyotype of Triticum longissimum and its cytogenetic relationship with T. aestivum. Genome 36:731–742
Friebe B, Jiang J, Tuleen N, Gill BS (1995) Standard karyotype of Triticum umbellulatum and the characterization of derived chromosome addition and translocation lines in common wheat. Theor Appl Genet 90:150–156
Furuta Y (1981) Intraspecific variation in Aegilops variabilis and Ae. kotschyi revealed by chromosome pairing in F1 hybrids. Jpn J Genet 56:495–504
Gill KS, Lubbers EL, Gill BS, Raupp WJ, Cox TS (1991) A genetic linkage map of Triticum tauschii (DD) and its relationship to the D genome of bread wheat (AABBDD). Genome 34:362–374
Hart GE, Tuleen NA (1983a) Chromosomal locations of eleven Elytrigia elongata (=Agropyron elongatum) isozyme structural genes. Genet Res 41:181–202
Hart GE, Tuleen NA (1983b) Characterizing and selecting alien genetic material in derivatives of wheat-alien species hybrids by analyses of isozyme variation. In: Proc 6th Int Wheat Genet Symp, Kyoto, Japan, pp 377–385
Jewell DC, Driscoll CJ (1983) The addition of Aegilops variabilis chromosomes to Triticum aestivum and their identification. Can J Genet Cytol 25:76–84
Kihara H (1940) Verwandtschaft der Aegilops-Arten im Lichte der Genomanalyse. Ein Uberblick Zuechter 12:49–62
Kimber G (1967) The addition of the chromosomes of Aegilops umbellulata to Triticum aestivum (var. Chinese Spring). Genet Res 9:111–114
Kimber G, Feldman M (1987) Wild wheat, an introduction. Special report 353. College of Agriculture, University of Missouri-Columbia
Kimber G, Yen Y (1989) Hybrids involving autotetraploid Triticum umbellulatum. Genome 32:1–5
King IP, Purdie KA, Liu CJ, Reader SM, Pittaway TS, Orford SE, Miller TE (1994) Detection of interchromosomal translocations within the Triticeae by RFLP analysis. Genome 37:882–887
Kleinhofs A, Kilian A, Saghai Maroof MA, Biyashev RM, Hayes P, Chen FQ, Lapitan N, Fenwick A, Blake TK, Kanazin V, Ananiev E, Dahleen L, Kudrna D, Bollinger J, Knapp SJ, Liu B, Sorrells M, Heun M, Franckowiak JD, Hoffman D, Skadsen R, Steffenson BJ (1993) A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome. Theor Appl Genet 86:705–712
Miller TE, Reader SM (1987) A guide to homoeology of chromosomes within the Triticeae. Theor Appl Genet 74:214–217, erratum 680
Morden CW, Doebley J, Schertz KF (1986) A manual of techniques for starch-gel electrophoresis of Sorghum isozymes. A miscellaneous publication of the Texas Agricultural Experiment Station, College Station
Riley R, Chapman V, Johnson R (1968) The incorporation of alien disease resistance in wheat by genetic interference with the regulation of meiotic synapsis. Genet Res 12:199–219
Sharp PJ, Chao S, Desai S, Gale MD (1989) The isolation, characterization and application in the Triticeae of a set of wheat RFLP probes identifying each homoeologous chromosome arm. Theor Appl Genet 78:342–348
Shepherd, KW, Islam, SKMR (1988) Fourth compendium of wheatalien chromosome lines. In: Miller TE, Koebner RMD, (eds) Proc 7th Int Wheat Genet Symp, Cambridge, England, pp 1373–1395
Tuleen NA, Hart GE (1988) Isolation and characterization of wheatElytrigia elongata chromosome 3E and 5E addition and substitution lines. Genome 30:289–292
Zhang H-B, Dvorak J, Waines JG (1992) Diploid ancestry and evolution of Triticum kotschyi and T. peregrinum examined using variation in repeated nucleotide sequences. Genome 35:182–191
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Communicated by J. W. Snape
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Yang, Y.C., Tuleen, N.A. & Hart, G.E. Isolation and identification of Triticum aestivum L. em. Thell. cv Chinese Spring-T. peregrinum Hackel disomic chromosome addition lines. Theoret. Appl. Genetics 92, 591–598 (1996). https://doi.org/10.1007/BF00224563
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DOI: https://doi.org/10.1007/BF00224563