Abstract
The root-knot nematode heat-stable resistance locus from L. peruvianum LA2157 was mapped on chromosome 6. All wild tomato LA2157 entries and the LA2157 S1 progeny tested were resistant to Mi-avirulent Meloidogyne spp. isolates at 32°C, indicating that the self-compatible accession is homozygous for heat-stable nematode resistance. The novel resistance locus was mapped on a RFLP linkage map; this map was based on a segregating F2 population obtained from the interspecific F1 between L. esculentum cv ‘Solentos’ and L. peruvianum LA2157. The inheritance of the heat-stable resistance was evaluated in 100 F3 lines derived from one F1 interspecific hybrid. The genotype of the resistance locus of the individual F2 plants was based on the phenotypic classification of their F3 lines, and the data were used to map the resistance locus on the arm of chromosome 6 with the closest linkage to TG178. The position of the novel heat-stable resistance of LA2157 was localized in the resistance genes’ cluster close to the location of gene Mi-1. Cuttings of the F3 lines expressed resistance to Mi-1-avirulent M. incognita and M. javanica biotypes at 25°C and at 32°C (a temperature at which Mi-1 resistance is not expressed). There was no difference in the segregating population for expression of heat-unstable resistance and heat-stable resistance to Mi-1-avirulent Meloidogyne spp. However, LA2157 and cuttings of the above F3 lines were susceptible to a Mi-1-virulent M. incognita isolate at 30°C and to a M. hapla isolate at 25°C.
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Received: 6 July 1998 / Accepted: 28 July 1998
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Veremis, J., van Heusden, A. & Roberts, P. Mapping a novel heat-stable resistance to Meloidogyne in Lycopersicon peruvianum. Theor Appl Genet 98, 274–280 (1999). https://doi.org/10.1007/s001220051068
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DOI: https://doi.org/10.1007/s001220051068