Summary
A needle-type glucose sensor has been developed using a platinum electrode covered with immobilized glucose oxidase. Experiments with albumin-saline solution in vitro showed that at 5.5 mmol/l glucose concentration the output current generated was 1.2±0.4 nA (mean ± SD). The current increased as a linear function of glucose concentration over the range (0–27.7 mmol/l). The response time to reach 90% of the final plateau value was 16.2±6.2 s. The signal-to-noise ratio of the sensor was 15.8±2.6 decibels. The temperature coefficient in output was 2.3±1.0%/°C. The current output was not affected significantly by changes in oxygen tension of the solution in the range 25–150 mmHg.
In vivo, the output current of sensors inserted into the subcutaneous tissues of dogs was directly related to blood glucose concentrations after oral glucose or meals. Daily checking of the sensors maintained in subcutaneous tissues in five dogs showed that the sensitivity decreased gradually to 87.2±7.6% at 72 h, and dropped significantly to 57.4±7.0% of the initial output by 96 h.
A wearable artificial endocrine pancreas (18.0 × 17.7 × 7.9 cm, 700 g) was developed, consisting of a needle-type glucose sensor, a microcomputer system and a pump driving mechanism. Three pancreatectomized dogs were fitted with the system by inserting the sensor into subcutaneous tissue. By renewing the sensor every fourth day, the device could maintain the daily glucose variations in diabetic dogs within the range 5–9.5 mmol/l for 7 days.
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References
Albisser AM, Leibel BS, Ewart TG, Davidovac Z, Botz CK, Zingg W, Shipper H, Gander R (1974) Clinical control of diabetes by the artificial pancreas. Diabetes 23: 394–404
Kawamori R, Shichiri M, Goriya Y, Yamasaki Y, Shigeta Y, Abe H (1978) Importance of insulin secretion based on the rate of change in blood glucose concentration in glucose tolerance, assessed by the artificial beta cell. Acta Endocrinol (Kbh) 87: 339–351
Shichiri M, Kawamori R, Abe H (1979) Normalization of paradoxic secretion of glucagon in diabetics who were controlled by the artificial beta cell. Diabetes 28: 272–275
Kawamori R, Shichiri M, Kikuchi M, Yamasaki Y, Abe H (1980) Perfect normalization of excessive glucagon responses to intravenous arginine in human diabetes mellitus with the artificial beta cell. Diabetes 29: 762–765
Clemens AH, Chang PH, Myers RW (1977) The development of Biostator, a glucose controlled insulin infusion system. Horm Metab Res 7 [Suppl]: 22–23
Zinman B, Stokes EF, Albisser AM, Hanna HL, Minuk AN, Stein AN, Leibel BS, Marliss EB (1979) The metabolic response to glycemic control by the artificial pancreas in diabetic man. Metabolism 28: 511–517
Mirouze J, Selam JL, Pham TC, Mendoza E, Orsetti A (1978) Sustained insulin induced remissions of juvenile diabetes by means of an external artificial pancreas. Diabetologia 14: 223–227
Nakagawa S, Nakayama H, Sasaki T, Yoshino K, Yu YY, Shinizaki K, Aoki S, Mashimo K (1973) A simple method for the determination of serum free insulin levels in insulin-treated patients. Diabetes 22: 590–600
Eayne EC, Schultz RD, Thomas LJ, Slama G, Sayler DF, Bessman SP (1976) Continuous extracorporeal monitoring of animal blood using the glucose electrode. Diabetes 25: 81–89
Wakabayashi A, Nakamura Y (1975) Continuous percutaneous monitoring of muscle pH and oxygen pressure: a new technique for in vivo use. Arch Surg 110: 802–805
Van Liew HD (1968) Oxygen and carbon dioxide tensions in tissue and blood of normal and acidotic rats. J Appl Physiol 25: 575–580
Kawamori R, Yamasaki Y, Murata T, Morishima T, Yagi T, Goriya Y, Shichiri M, Abe H (1980) Validation of self-adaptive control algorithm for blood glucose concentration in artificial beta cell. Automedia 3:183–189
Bessman SP, Hellyer JM, Layne EC, Takeda G, Thomas LJ, Sayler D (1977) The total implantation of an artificial B-cell in a dog: progress report. In: JS Bajaj (ed) Excerpta Medica International Congress Series 413: pp 496–501
Schlichtkrull J, Munck O, Jersild M (1965) The M-value, an index of blood-sugar control in diabetics. Acta Medica Scand 177: 95–102
Service FJ, Molnar GD, Rosevear JW, Ackerman E, Gatewood LC, Taylor WF (1970) Mean amplitude of glycemic excursions, a measure of diabetic instability. Diabetes 19: 644–655
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Shichiri, M., Kawamori, R., Goriya, Y. et al. Glycaemic control in pancreatectomized dogs with a wearable artificial endocrine pancreas. Diabetologia 24, 179–184 (1983). https://doi.org/10.1007/BF00250158
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DOI: https://doi.org/10.1007/BF00250158