Summary
A radioimmunoassay using antibody against glucitol-lysine was developed to quantitate glycated proteins in the lens of diabetic rats. The amount of glycated protein was expressed as molar equivalents of reduced glycated hippuryl lysine (GlcRED-Hip-Lysine). Significant differences (p<0.01) were found in the amounts of glycated protein in the lenses of rats with streptozotocin-induced diabetes (3.92±0.59 nmol/mg protein, n=5), those with streptozotocin-induced diabetes treated with insulin (2.94±0.36 nmol/mg protein, n=4) and normal rats (1.23±0.22 nmol/mg protein, n=5). There was a significant correlation between the concentration of glycated protein in the lens and the HbA1c level at the end of the 12 week experiment (r=0.957, p<0.001). These results indicate that glycation of lens protein is parallel with the severity of diabetes in rats.
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Yano, M., Hashimoto, F., Sato, M. et al. Specific radioimmunoassay of glucitol-lysine — application to lens proteins in streptozotocin-diabetic rats. Diabetologia 31, 221–224 (1988). https://doi.org/10.1007/BF00290588
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DOI: https://doi.org/10.1007/BF00290588