Summary
The basal and total pyruvate dehydrogenase activities were assayed in circulating lymphocytes from children with juvenile diabetes at diagnosis and after five days of insulin therapy and from control subjects. In untreated diabetic children, basal and total pyruvate dehydrogenase activities were deeply decreased and both showed very similar values; whereas, in control subjects basal activity was about 30% lower than total activity. In diabetic patients treated with insulin (in vivo situation), both basal and total activity levels were equal or even higher than those of the control subjects. The incubation of lymphocytes from diabetic patients with insulin (5 μU/ml) (in vitro situation) stimulates, but less than in vivo, the basal and total pyruvate dehydrogenase activities.
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Wieland OH (1983) The mammalian pyruvate dehydrogenase complex: Structure and regulation. Rev Physiol Biochem Pharmacol 96: 123–170
Curto M, Piccinini M, Bruno R, Mostert M, Rinaudo MT (1988) Insulin modulation of pyruvate dehydrogenase in human circulating lymphocytes. Int J Biochem 20: 1211–1217
Larner J, Cheng K, Scharwtz C, Kikuchi K, Tamura S, Creacy S, Dubler R, Galasko G, Pullin C, Katz M (1982) Insulin mediators and their control of metabolism through protein phosphorylation. Recent Prog Horm Res 38: 511–552
Rinaudo MT, Curto M, Bruno R (1986) Effect of insulin on pyruvate dehydrogenase in a mixture of plasma membranes and mitocondria from normal and alloxan treated rat brains. Int J Biochem 18: 603–607
Rinaudo MT, Bedino S, Curto M, Testore G, Bruno R (1986) Characterization of a pyruvate dehydrogenase modulator purified from insulin treated rat brain plasma membranes. It J Biochem 35: 242–258
Rinaudo MT, Curto M, Bruno R, Marino C, Rossetti V, Mostert M (1987) Evidence of an insulin generated pyruvate dehydrogenase stimulating factor in rat brain plasma membranes. Int J Biochem 19: 909–913
Rinaudo MT, Curto M, Bruno R, Marino C, Mostert M (1988) Effects of insulin on pyruvate dehydrogenase in circulating lymphocytes from normal and diabetic rats. Int J Biochem 20: 667–674
Boyüm A (1968) Separation of leucocytes from blood and bone marrow. Scand J Clin Lab Invest 21: 9–89
Blass JP, Avigan J, Uhlendorf BW (1970) A defect in pyruvate decarboxylase in a child with an intermittent movement disorder. J Clin Invest 49: 423–432
Janossy G, Bollum FJ, Bradstock KF, Ashley J (1980) Cellular phenotypes of normal and leukemic hemopoietic cells determined by analysis with selected monoclonal antibody combinations. Blood 56: 430–441
Lennert K, Mohri N, Stein H, Kaiserling E, Müller-Hermelink HK (1978) Malignant lymphomas other than Hodgkin's disease. In: Lennert K (ed) Handbuch der Speziellen Pathologischen Anatomie und Histologie, Bd 1, Teil 3B. Springer, Berlin Heidelberg New York, pp 9–19
Mueller J, Brun Del Re G, Buerki H, Keller HU, Hess MW, Cottier H (1975) Non specific esters activity: a criterion for differentiation of T and B lymphocytes in mouse lymph-nodes. Eur J Immunol 5: 270–274
Rinaudo MT, Curto M, Bruno R (1985) Effect of insulin on pyruvate dehydrogenase complex in the rat brain. It J Biochem 34: 229–237
Wieland OH, Siess E, Schulze-Wethmar FH, Von Funcke HG, Winton B (1971) Active and inactive forms of pyruvate dehydrogenase in rat heart and kidney. Effect of diabetes, fasting and refeeding on pyruvate dehydrogenase interconversion. Arch Biochem Biophys 143: 593–601
Wieland OH, Siess EA, Weiss L, Löffler G, Patzelt C, Portenhauser R, Hartmann V, Schirmann A (1973) Regulation of mammalian pyruvate dehydrogenase complex by covalent modifications. Symp Soc Exp Biol 27: 371–400
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Rinaudo, M.T., Curto, M., Bruno, R. et al. The behaviour of pyruvate dehydrogenase in circulating lymphocytes from diabetic children. Diabetologia 32, 245–248 (1989). https://doi.org/10.1007/BF00285292
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DOI: https://doi.org/10.1007/BF00285292