Summary
Muscle specimens from the quadriceps femoris muscle were obtained from eight healthy subjects by means of an open muscle biopsy and prepared for in vitro incubation. C-peptide at 0.5, 1.0 and 2.5 nmol/l increased 3-0-methylglucose transport by 38% (NS), 64% (p<0.05), and 64 % (p<0.05) respectively. Glucose transport increased 1.8-fold in the presence of 0.3 nmol/l of insulin (p<0.05). Glycogen content in muscle strips exposed to C-peptide at a concentration of 1 nmol/l increased significantly by 22% (p<0.05). In conclusion, C-peptide stimulates the rate of 3-0-methylglucose transport in in vitro incubated human skeletal muscle strips in a dose-response manner. These observations suggest that C-peptide may contribute to the regulation of carbohydrate metabolism in human skeletal muscle.
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Zierath, J.R., Galuska, D., Johansson, B.L. et al. Effect of human C-peptide on glucose transport in in vitro incubated human skeletal muscle. Diabetologia 34, 899–901 (1991). https://doi.org/10.1007/BF00400197
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DOI: https://doi.org/10.1007/BF00400197