Summary
Blood lipid values were determined in 48 normal, 56 subdiabetic and 6 overtly alloxan-diabetic rabbits before and after the initiation of experimental hypercholesterolaemia. Subdiabetes, induced by small doses of alloxan or by sequentially administered injections of cortisone and alloxan, is characterized by normoglyaemia changing to hyperglycaemia following repeated, normally nondiabetogenic small cortisone injections. Before cholesterol feeding, the subdiabetic rabbits attained considerably higher cholesterol, phospholipid, nonesterified fatty acid and total lipid levels than did the metabolically normal animals. The corresponding prefeeding levels of the alloxan-diabetic rabbits were also higher than normal, but their average cholesterol and phospholipid values remained lower than in the subdiabetic animals. During cholesterol feeding, the averages of all lipid fractions, especially in the subdiabetic and alloxan-diabetic animals, rose considerably for 8 weeks; thereafter, they tended to decline but continued to remain abnormally high, and only minor differences between the experimental groups persisted.
Résumé
Les valeurs des lipides sanguins ont été déterminées chez 48 lapins normaux, 56 lapins sub-diabétiques et 6 lapins manifestement diabétiques par l'alloxane, avant et après l'induction d'une hypercholestérolémie expérimentale. Le sub-diabète, provoqué par de petites doses d'alloxane, ou par des injections séquentielles de cortisone et d'alloxane, est caractérisé par une glycémie normale évoluant vers l'hyperglycémie à la suite de petites injections répétées de cortisone normalement non-diabétogènes. Avant l'administration de cholestérol, les lapins sub-diabétiques atteignaient des taux considérablement plus élevés de cholestérol, de phospholipides, d'acides gras non-estérifiés et de lipides totaux que les animaux métaboliquement normaux. Les taux correspondants (avant l'administration de cholestérol) étaient également plus élevés chez les lapins diabétiques par l'alloxane que chez les lapins normaux, mais leurs valeurs moyennes de cholestérol et de phospholipides étaient plus basses que chez les animaux sub-diabétiques. Au cours de l'administration de cholestérol, les moyennes de toutes les fractions lipidiques, spécialement chez les animaux sub-diabétiques et diabétiques par l'alloxane, augmentaient considérablement pendant 8 semaines; par la suite elles avaient tendance à diminuer mais continuaient à rester anormalement élevées, et seulement de petites différences persistaient entre les groupes expérimentaux.
Zusammenfassung
Bei 48 gesunden, 56 subdiabetischen und sechs alloxan-diabetischen Kaninchen wurden die Blutlipoidwerte vor und während einer experimentell induzierten Hypercholesterinämie bestimmt. Subdiabetes wurde mit kleinen Alloxandosen oder mit einer Kombination von Cortison und Alloxan erzeugt; subdiabetische Tiere werden nur nach zusätzlichen, normalerweise nicht diabetogenen kleinen Cortisondosen hyperglykämisch. Bereits vor der Verfütterung von Cholesterin wiesen die subdiabetischen Kaninchen deutlich erhöhte Cholesterin-, Phospholipoid-, Fettsäure-und Gesamtlipoidwerte im Blut auf. Auch bei den alloxandiabetischen Tieren waren die entsprechenden Werte erhöht, beim Cholesterin und den Phospholipoiden jedoch weniger eindeutig. Während der ersten acht Wochen der Cholesterinverabreichung stiegen alle Lipoidfraktionen, vor allem bei den subdiabetischen und alloxandiabetischen Tieren, stark an. Danach zeigte sich eine leicht abfallende Tendenz, wobei sich die Unterschiede zwischen den experimentellen Gruppen gleichzeitig verringerten.
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Wellmann, K.F., Volk, B.W. Blood lipids in normal and subdiabetic rabbits before and after the induction of hypercholesterolaemia. Diabetologia 7, 189–194 (1971). https://doi.org/10.1007/BF01212552
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DOI: https://doi.org/10.1007/BF01212552